IS408
- Family IS21
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AB182267 | Y | Burkholderia cepacia | Burkholderia cepacia ATCC17616 plasmid pTGL1 Burkholderia multivorans ATCC17616 |
DNA section
IS Length : 2798 bp
Ends
IR Length : 39/47
IRL : TGCGTATTCCGGCGAACGTGATCACGCATTTCGGCGGATCGTGATCATGG
IRR : TGCGGATTTCGGCGATGGTGATCAATCGTTTCGGCGAACGTGATCAGTCT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GTATGTGGTG | GATGAAGC | AATATTGAAA | 8 |
NNNNNNNNNN | GCTGATA | NNNNNNNNNN | 7 |
DNA sequence
TGCGTATTCCGGCGAACGTGATCACGCATTTCGGCGGATCGTGATCATGGATTCCGAATGATCGTGATCACCCGTTTCGGTTGATCGTGATCGCTGATCA
CAGGTTTCCGAAACGCGTGATCACGATGCCGGAATGAGCGATCACGGGACCGAAACGGGTGATCACGTAACCGGAACGTAATCCGGGTTTGAGTCTGGCG
GTGGCGTTGCGCATGTATTCACCGACGGCGTTAGCCTGCTCCCTTTTTTGCGGGAGAAGTGCCGATGCCGACACCTCGGATGAGCATGCGCAAATTGAAG
GAAGTCCTGCGACTAAAGTGGGCGTGTGGCCTGACGCATCGGCAGATCAGCCGGGCTATCGGCATCAGCGTCGGTGCCGTCTCGAAGTTCGCGGCCCAAG
CCAGCCAGGCGGGACTCGATTGGGCGGCAGCCGAAGCAATGTCCGACGACGAACTGGACGCGCGCCTGAGGCCTGCGGCAACCAATGCCGCGGCGACGAC
GACGCGACGCATCGAGCCCGACTACACGGCATTGCATCGCGAGCTGCGCCGCAAGGGCGTGACGCTGCAACTGCTGTGGGAAGAATACGCTGAAGCGAAC
CCCGGCCAGCGGACGTACCGCTACACGCAGTTCTGCCAGAAGTACAAGGATTGGGCGAAGTCGATCAAGCGCTCGATGCGTCAGCAGCACCGCGCCGGCG
AGAAGCTGTTCGCGGACTTCGCGGGTCCCACCGTGCCGGTCCTGGCGAGCGATGGCGGCGTCGAGTTCGAGGCGCACGTGTTCGTTGCCGTGCTCGGCGC
ATCCAACTACACGTTCGCGTGCGCGACGCGCACCGAGACGATGGCGGACTGGATCGGCAGCCTGTGCGATGCGCTAGAGTTCATTGGCGGCGTGCCCGAG
CTGCTCGTGCCCGACAACCCGAAGGCGCTGATCGCGCGACCCGACCGTTACGAACCCGGACTCGGCACGACCACGCAGGACTTCGTGAACCACTACGGCA
CCGCGATGCTGCCGGCGCGTCCGCGCAAACCGCAGGACAAGGCCAAGGTCGAAGTCGGCGTGCAGATCGTCGAGCGCTGGGTGCTGGCCCGACTGCGCCA
CTATCGCTTCTACAGTCTGGCTGAACTCAACAAGGCGATTGCAGAACTGATCGCCGACCTAAACCAGCGCCCGTTCAAGCGGCTCGAAGGCAACCGGCGT
GAATGGTTCGAACGGCTGGACCAACCCGTCCTGCGCCCGTTGCCCGTGCGTCGCTACGAGATCGCCACCTTCCAGAAGTGCCGTGTGAACATCGATTACC
ACGTCGATGTCGGCGGCCACTATTACAGCGTCCCGCATAGCCTCGCACGGCAGGAGGTCTGGGCGCGTATCACACGCCACGGCGTAGAGATCCTGCATGG
CGGCAAGCGTGTCGCTGCGCACGCTCGCTCGCGCCTCAAAGGCAAACACACGACCATCGCCGAACACATGCCGGCTGCGCATCGCGCGCACATGGAATGG
ACGCCTGGGCGCCTGCTCAACTGGGGCGCATCCGTCGGCCCCGGCGCCGAGGCTGTCGTGCGGCATCTGCTGACCAACAAACCTCACCCCGAGATGGGCT
ATCGCGCCTGTCTCGGTCTTCTCTCGCTCGCGCGCAAGTACGGCAAACACCGCCTCGAGGCCGCATGCCAGCGTGCATTGAAGATCGGCTCGCCCACGCG
TCGCTCCGTGCTGTCAATCCTGGAAGCGGGACTTGACCTGCAGCCCTCGCTCCCGACCACGCCCGCCGAATGGCGCTCGCCCGAGCACGAGAACGTGCGC
GGCCCCGACTACTACCACTGACCCGAAGGAGGTCACGATGTTGATGCAGCACACCCTTACCCAGCTAAAGGCGCTCAAACTGGACGGCATGGCTCGCGCG
TTCGAGGAGCAGTCAACGCTCACGGCAAGCACCAGCCTGTCATTCGAGGAACGCGTGGGCATGCTCGTAGATCGCGAGCTGGCCTGGCGTGATACGCGAC
GACTCGAACGTTTGCTGCGCGCCGCGAAGCTCAAGAACCCACAGGCCTGCGTCGAAGACATTGAATACCGGCAAACCCGTGGGCTGGACCAGCGCATCGT
CGCCACGCTCGCCGGCTGCGACTGGGTGCGCAATGCTCAGAACCTGATCCTGACCGGCCCCACCGGCGCGGGCAAGACGTGGCTCGCTTGCGCGTTCGGC
CAGCAGGCGTGCCGGCAGGGCTTCTCCGTGTTCTATGTGCGCGTCGCGCGGCTGTTCGAAGAGCTGAAAATCGCGCACGGCGACGGCAGCTTCACACGTC
GGCTTGCGCAACTCGCGAAGATTGATGTGCTGATCCTCGACGATTGGGGCTTGCAGGATCTCGACCAGGCGGCGCGCAATGATCTGCTTGAGGTACTTGA
TGATCGTGTCGGTACGCGCTCGACTGTGATCACCAGCCAGTTGCCGCTCGAACACTGGCACGCGTGGCTGCAGGACCCCACGCTCGCCGATGCGATTCTC
GATCGGCTCGTGCATCAGGCCCACAAGCTTCCGGTCAAGGGCGATTCGATGAGAAAGAAAACGAAATCCGAGTCCGCTGACGCCTGATCGTGATCACCGT
CGCTATAATCCATTGACCGCGCAACACCACCTTCCAGACTGATCACGTTCGCCGAAACGATTGATCACCATCGCCGAAATCCGAGTCCGCTGACGCCTGA
TCGTGATCACCGTCGCTATAATCCATTGACCGCGCAACACCACCTTCCAGACTGATCACGTTCGCCGAAACGATTGATCACCATCGCCGAAATCCGCA
CAGGTTTCCGAAACGCGTGATCACGATGCCGGAATGAGCGATCACGGGACCGAAACGGGTGATCACGTAACCGGAACGTAATCCGGGTTTGAGTCTGGCG
GTGGCGTTGCGCATGTATTCACCGACGGCGTTAGCCTGCTCCCTTTTTTGCGGGAGAAGTGCCGATGCCGACACCTCGGATGAGCATGCGCAAATTGAAG
GAAGTCCTGCGACTAAAGTGGGCGTGTGGCCTGACGCATCGGCAGATCAGCCGGGCTATCGGCATCAGCGTCGGTGCCGTCTCGAAGTTCGCGGCCCAAG
CCAGCCAGGCGGGACTCGATTGGGCGGCAGCCGAAGCAATGTCCGACGACGAACTGGACGCGCGCCTGAGGCCTGCGGCAACCAATGCCGCGGCGACGAC
GACGCGACGCATCGAGCCCGACTACACGGCATTGCATCGCGAGCTGCGCCGCAAGGGCGTGACGCTGCAACTGCTGTGGGAAGAATACGCTGAAGCGAAC
CCCGGCCAGCGGACGTACCGCTACACGCAGTTCTGCCAGAAGTACAAGGATTGGGCGAAGTCGATCAAGCGCTCGATGCGTCAGCAGCACCGCGCCGGCG
AGAAGCTGTTCGCGGACTTCGCGGGTCCCACCGTGCCGGTCCTGGCGAGCGATGGCGGCGTCGAGTTCGAGGCGCACGTGTTCGTTGCCGTGCTCGGCGC
ATCCAACTACACGTTCGCGTGCGCGACGCGCACCGAGACGATGGCGGACTGGATCGGCAGCCTGTGCGATGCGCTAGAGTTCATTGGCGGCGTGCCCGAG
CTGCTCGTGCCCGACAACCCGAAGGCGCTGATCGCGCGACCCGACCGTTACGAACCCGGACTCGGCACGACCACGCAGGACTTCGTGAACCACTACGGCA
CCGCGATGCTGCCGGCGCGTCCGCGCAAACCGCAGGACAAGGCCAAGGTCGAAGTCGGCGTGCAGATCGTCGAGCGCTGGGTGCTGGCCCGACTGCGCCA
CTATCGCTTCTACAGTCTGGCTGAACTCAACAAGGCGATTGCAGAACTGATCGCCGACCTAAACCAGCGCCCGTTCAAGCGGCTCGAAGGCAACCGGCGT
GAATGGTTCGAACGGCTGGACCAACCCGTCCTGCGCCCGTTGCCCGTGCGTCGCTACGAGATCGCCACCTTCCAGAAGTGCCGTGTGAACATCGATTACC
ACGTCGATGTCGGCGGCCACTATTACAGCGTCCCGCATAGCCTCGCACGGCAGGAGGTCTGGGCGCGTATCACACGCCACGGCGTAGAGATCCTGCATGG
CGGCAAGCGTGTCGCTGCGCACGCTCGCTCGCGCCTCAAAGGCAAACACACGACCATCGCCGAACACATGCCGGCTGCGCATCGCGCGCACATGGAATGG
ACGCCTGGGCGCCTGCTCAACTGGGGCGCATCCGTCGGCCCCGGCGCCGAGGCTGTCGTGCGGCATCTGCTGACCAACAAACCTCACCCCGAGATGGGCT
ATCGCGCCTGTCTCGGTCTTCTCTCGCTCGCGCGCAAGTACGGCAAACACCGCCTCGAGGCCGCATGCCAGCGTGCATTGAAGATCGGCTCGCCCACGCG
TCGCTCCGTGCTGTCAATCCTGGAAGCGGGACTTGACCTGCAGCCCTCGCTCCCGACCACGCCCGCCGAATGGCGCTCGCCCGAGCACGAGAACGTGCGC
GGCCCCGACTACTACCACTGACCCGAAGGAGGTCACGATGTTGATGCAGCACACCCTTACCCAGCTAAAGGCGCTCAAACTGGACGGCATGGCTCGCGCG
TTCGAGGAGCAGTCAACGCTCACGGCAAGCACCAGCCTGTCATTCGAGGAACGCGTGGGCATGCTCGTAGATCGCGAGCTGGCCTGGCGTGATACGCGAC
GACTCGAACGTTTGCTGCGCGCCGCGAAGCTCAAGAACCCACAGGCCTGCGTCGAAGACATTGAATACCGGCAAACCCGTGGGCTGGACCAGCGCATCGT
CGCCACGCTCGCCGGCTGCGACTGGGTGCGCAATGCTCAGAACCTGATCCTGACCGGCCCCACCGGCGCGGGCAAGACGTGGCTCGCTTGCGCGTTCGGC
CAGCAGGCGTGCCGGCAGGGCTTCTCCGTGTTCTATGTGCGCGTCGCGCGGCTGTTCGAAGAGCTGAAAATCGCGCACGGCGACGGCAGCTTCACACGTC
GGCTTGCGCAACTCGCGAAGATTGATGTGCTGATCCTCGACGATTGGGGCTTGCAGGATCTCGACCAGGCGGCGCGCAATGATCTGCTTGAGGTACTTGA
TGATCGTGTCGGTACGCGCTCGACTGTGATCACCAGCCAGTTGCCGCTCGAACACTGGCACGCGTGGCTGCAGGACCCCACGCTCGCCGATGCGATTCTC
GATCGGCTCGTGCATCAGGCCCACAAGCTTCCGGTCAAGGGCGATTCGATGAGAAAGAAAACGAAATCCGAGTCCGCTGACGCCTGATCGTGATCACCGT
CGCTATAATCCATTGACCGCGCAACACCACCTTCCAGACTGATCACGTTCGCCGAAACGATTGATCACCATCGCCGAAATCCGAGTCCGCTGACGCCTGA
TCGTGATCACCGTCGCTATAATCCATTGACCGCGCAACACCACCTTCCAGACTGATCACGTTCGCCGAAACGATTGATCACCATCGCCGAAATCCGCA
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1557 bp | 518 aa | 265 | 1821 | + | No |
Chemistry : DDE
ORF sequence :
MPTPRMSMRKLKEVLRLKWACGLTHRQISRAIGISVGAVSKFAAQASQAGLDWAAAEAMSDDELDARLRPAATNAAATTTRRIEPDYTALHRELRRKGVT
LQLLWEEYAEANPGQRTYRYTQFCQKYKDWAKSIKRSMRQQHRAGEKLFADFAGPTVPVLASDGGVEFEAHVFVAVLGASNYTFACATRTETMADWIGSL
CDALEFIGGVPELLVPDNPKALIARPDRYEPGLGTTTQDFVNHYGTAMLPARPRKPQDKAKVEVGVQIVERWVLARLRHYRFYSLAELNKAIAELIADLN
QRPFKRLEGNRREWFERLDQPVLRPLPVRRYEIATFQKCRVNIDYHVDVGGHYYSVPHSLARQEVWARITRHGVEILHGGKRVAAHARSRLKGKHTTIAE
HMPAAHRAHMEWTPGRLLNWGASVGPGAEAVVRHLLTNKPHPEMGYRACLGLLSLARKYGKHRLEAACQRALKIGSPTRRSVLSILEAGLDLQPSLPTTP
AEWRSPEHENVRGPDYYH
LQLLWEEYAEANPGQRTYRYTQFCQKYKDWAKSIKRSMRQQHRAGEKLFADFAGPTVPVLASDGGVEFEAHVFVAVLGASNYTFACATRTETMADWIGSL
CDALEFIGGVPELLVPDNPKALIARPDRYEPGLGTTTQDFVNHYGTAMLPARPRKPQDKAKVEVGVQIVERWVLARLRHYRFYSLAELNKAIAELIADLN
QRPFKRLEGNRREWFERLDQPVLRPLPVRRYEIATFQKCRVNIDYHVDVGGHYYSVPHSLARQEVWARITRHGVEILHGGKRVAAHARSRLKGKHTTIAE
HMPAAHRAHMEWTPGRLLNWGASVGPGAEAVVRHLLTNKPHPEMGYRACLGLLSLARKYGKHRLEAACQRALKIGSPTRRSVLSILEAGLDLQPSLPTTP
AEWRSPEHENVRGPDYYH
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
750 bp | 249 aa | 1838 | 2587 | + | No |
AG : IS21 helper
ORF sequence :
MLMQHTLTQLKALKLDGMARAFEEQSTLTASTSLSFEERVGMLVDRELAWRDTRRLERLLRAAKLKNPQACVEDIEYRQTRGLDQRIVATLAGCDWVRNA
QNLILTGPTGAGKTWLACAFGQQACRQGFSVFYVRVARLFEELKIAHGDGSFTRRLAQLAKIDVLILDDWGLQDLDQAARNDLLEVLDDRVGTRSTVITS
QLPLEHWHAWLQDPTLADAILDRLVHQAHKLPVKGDSMRKKTKSESADA
QNLILTGPTGAGKTWLACAFGQQACRQGFSVFYVRVARLFEELKIAHGDGSFTRRLAQLAKIDVLILDDWGLQDLDQAARNDLLEVLDDRVGTRSTVITS
QLPLEHWHAWLQDPTLADAILDRLVHQAHKLPVKGDSMRKKTKSESADA
Blast result :
Comments
IS408 was originally detected in Burkholderia cepacia as an insertion into the lacZ gene of Tn951, on the broad host-range plasmid pGC91.14 (Wood et al., 1990). On pTGL5, IS408 is located 91 bp from IS401. Although both ends of been sequenced, less than 5 % of IS408 remains to be determined, at the 3' end of the element (Byrne and Lessie, 1994). IS408 contains at least three 23-bp terminal repeats (L1, L2, L3, R1 and R2):
L1: TTCCGGCGAACGTGATCACGCAT (7-29)
L2: TTCGGCGGATCGTGATCATGGAT (30-52)
L3: TTCGGTTGATCGTGATCGCTGAT (76-98)
R1: TTTCGGTGATGGTGATCAGGCGT (7-29) CS
R2: TTCGGCGAACGTGATCAGTTTG (30-51) CS
L1: TTCCGGCGAACGTGATCACGCAT (7-29)
L2: TTCGGCGGATCGTGATCATGGAT (30-52)
L3: TTCGGTTGATCGTGATCGCTGAT (76-98)
R1: TTTCGGTGATGGTGATCAGGCGT (7-29) CS
R2: TTCGGCGAACGTGATCAGTTTG (30-51) CS
References
1] Gaffney, T.D., and Lessie, T.G. (1987) J. Bacteriol. 169, 224-230.
2] Wood, M.S., Lory, C., and Lessie, T.G. (1990) J. Bacteriol. 172, 1719-1724
3] Byrne, AM., and Lessie, T.G. (1994) Plasmid 31, 138-147.
4] Yoshiyuki Ohtsubo, Hiroyuki Genka, Harunobu Komatsu, Yuji Nagata, and Masataka Tsuda (2004)
Applied and Environmental Microbiology, in preparation. High-Temperature-Induced Transposition of IS Elements in Burkholderia multivorans ATCC 17616
2] Wood, M.S., Lory, C., and Lessie, T.G. (1990) J. Bacteriol. 172, 1719-1724
3] Byrne, AM., and Lessie, T.G. (1994) Plasmid 31, 138-147.
4] Yoshiyuki Ohtsubo, Hiroyuki Genka, Harunobu Komatsu, Yuji Nagata, and Masataka Tsuda (2004)
Applied and Environmental Microbiology, in preparation. High-Temperature-Induced Transposition of IS Elements in Burkholderia multivorans ATCC 17616