IS406
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M83145 | Y | Burkholderia cepacia | Burkholderia cepacia ATCC17616 |
DNA section
IS Length : 1367 bp
Ends
IR Length : 22/27
IRL : GTTGTGTATCGTTCTTTCTGTAAATTCCGTTTTGCCCGTGGCCTGCTGGG
IRR : GATTGTGCAACCTCTTTTCTGTAAATTTCCGGATTGGCGGTCATGACATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTCCGACTC | GTATGTTG | TGTGAAATTG | 8 |
DNA sequence
GTTGTGTATCGTTCTTTCTGTAAATTCCGTTTTGCCCGTGGCCTGCTGGGCGGGGAGCGTAGGCGCGTAGCGCCGGAGGTCACCGCCCAGCAGGCGGCAC
GACGGTGGGTCTGACGCCATGATTTCGAGAAAGGAGTGTATCCCTCATGGCGATGCGTGTCGAAACGAATCCTTTGGAAGCCGCCTATGCCGCCCTGCTT
GAAAATGGCCTGGACGGCGCCGGCGAAGCCCTGCGTATCCTCGTCAATGAAGCCGCCAAGATCGAGCGAAGCGCGTTTCTCGGCGCCCGTCCCTACGAGC
GTACCGAAACACGGCGCGATTACGCCAATGGTTTCAAGCCCAAGACCGTGCTCACCCGGCATGGCGAATTGACCTTCCAGGTGCCGCAAGTGCGTTCCAG
CGACTTTTATCCTTCCGCCCTGGAGAAGGGCACCCGCACCGACCAGGCGGTCAATCTCGCCCTGGCCGAGATGTATGTCCAAGGCGTCTCCACCCGCCGC
GTCATCGACGTCCTGCAGCGCCTGCTCGGACCGGAGATTTCGCTTTCCAGCGCCCAGGTCAGCCGCGCTGCGGCCAAGCTCGACGAGGGACTCAGGGCTT
GGCGCGAACGTCCCTTGGGTGAGACGCCTTACCTTTTTCTGGATGCGCGCTACGAGAAGGTCCGCCTGGAAGGCCGCATCGTCGATTGCGCGGTTTTGAT
CGCTGTCGGCATCGAAGCTTCCGGCAAACGCCGTGTGCTGGGCTGCGAGGTCGCCACCTCGGAAGCCGAGATCAACTGGCGCCGTTTCCTGGAGAGCCTC
CTGGCCCGCGGCCTGAAGGGCGTCACGCTCATCATCGCCGACGATCATGCCGGACTCAAGGCGGCCCGCCGGGCCGTACTCCCCTCCGTGCCCTGGCAGC
GCTGCCAGTTCCATCTGCAGCAGAACGCCGGCGCGCTCACTACCCGGCAGGAGGCCCGAAAGACGGTGGCCGCCCAGATGCGCGCCATCTTCAACGCCCC
CGACCGGACGGAGGCCGAACGGCTCTTGAAGGCCGCTCTGACCCTCTGGTGCAAGGAACATCCGAAGCTTGCCGAATGGGCCGAGACGGCCATCCCTGAG
AGCCTGACCGTGTTCGACTTCCCGGCGGCGCATCGCATCCGGCTGCGCACCACCAATGGCCTGGAACGGATCAACCGGGAGCTGCGCCGACGCACCCGGG
TGGCGAGCATCTTCCCCAACCCCGATTCCTGCCTGCGCCTTGTCTCCGCACTGCTCGCCGAACTCGACGACGAGTGGATGACCGGCAAGGTCTATCTCAA
CTTCAACCCGTAACCCGAATGTCATGACCGCCAATCCGGAAATTTACAGAAAAGAGGTTGCACAATC
GACGGTGGGTCTGACGCCATGATTTCGAGAAAGGAGTGTATCCCTCATGGCGATGCGTGTCGAAACGAATCCTTTGGAAGCCGCCTATGCCGCCCTGCTT
GAAAATGGCCTGGACGGCGCCGGCGAAGCCCTGCGTATCCTCGTCAATGAAGCCGCCAAGATCGAGCGAAGCGCGTTTCTCGGCGCCCGTCCCTACGAGC
GTACCGAAACACGGCGCGATTACGCCAATGGTTTCAAGCCCAAGACCGTGCTCACCCGGCATGGCGAATTGACCTTCCAGGTGCCGCAAGTGCGTTCCAG
CGACTTTTATCCTTCCGCCCTGGAGAAGGGCACCCGCACCGACCAGGCGGTCAATCTCGCCCTGGCCGAGATGTATGTCCAAGGCGTCTCCACCCGCCGC
GTCATCGACGTCCTGCAGCGCCTGCTCGGACCGGAGATTTCGCTTTCCAGCGCCCAGGTCAGCCGCGCTGCGGCCAAGCTCGACGAGGGACTCAGGGCTT
GGCGCGAACGTCCCTTGGGTGAGACGCCTTACCTTTTTCTGGATGCGCGCTACGAGAAGGTCCGCCTGGAAGGCCGCATCGTCGATTGCGCGGTTTTGAT
CGCTGTCGGCATCGAAGCTTCCGGCAAACGCCGTGTGCTGGGCTGCGAGGTCGCCACCTCGGAAGCCGAGATCAACTGGCGCCGTTTCCTGGAGAGCCTC
CTGGCCCGCGGCCTGAAGGGCGTCACGCTCATCATCGCCGACGATCATGCCGGACTCAAGGCGGCCCGCCGGGCCGTACTCCCCTCCGTGCCCTGGCAGC
GCTGCCAGTTCCATCTGCAGCAGAACGCCGGCGCGCTCACTACCCGGCAGGAGGCCCGAAAGACGGTGGCCGCCCAGATGCGCGCCATCTTCAACGCCCC
CGACCGGACGGAGGCCGAACGGCTCTTGAAGGCCGCTCTGACCCTCTGGTGCAAGGAACATCCGAAGCTTGCCGAATGGGCCGAGACGGCCATCCCTGAG
AGCCTGACCGTGTTCGACTTCCCGGCGGCGCATCGCATCCGGCTGCGCACCACCAATGGCCTGGAACGGATCAACCGGGAGCTGCGCCGACGCACCCGGG
TGGCGAGCATCTTCCCCAACCCCGATTCCTGCCTGCGCCTTGTCTCCGCACTGCTCGCCGAACTCGACGACGAGTGGATGACCGGCAAGGTCTATCTCAA
CTTCAACCCGTAACCCGAATGTCATGACCGCCAATCCGGAAATTTACAGAAAAGAGGTTGCACAATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1167 bp | 388 aa | 147 | 1313 | + | No |
Chemistry : DDE
ORF sequence :
MAMRVETNPLEAAYAALLENGLDGAGEALRILVNEAAKIERSAFLGARPYERTETRRDYANGFKPKTVLTRHGELTFQVPQVRSSDFYPSALEKGTRTDQ
AVNLALAEMYVQGVSTRRVIDVLQRLLGPEISLSSAQVSRAAAKLDEGLRAWRERPLGETPYLFLDARYEKVRLEGRIVDCAVLIAVGIEASGKRRVLGC
EVATSEAEINWRRFLESLLARGLKGVTLIIADDHAGLKAARRAVLPSVPWQRCQFHLQQNAGALTTRQEARKTVAAQMRAIFNAPDRTEAERLLKAALTL
WCKEHPKLAEWAETAIPESLTVFDFPAAHRIRLRTTNGLERINRELRRRTRVASIFPNPDSCLRLVSALLAELDDEWMTGKVYLNFNP
AVNLALAEMYVQGVSTRRVIDVLQRLLGPEISLSSAQVSRAAAKLDEGLRAWRERPLGETPYLFLDARYEKVRLEGRIVDCAVLIAVGIEASGKRRVLGC
EVATSEAEINWRRFLESLLARGLKGVTLIIADDHAGLKAARRAVLPSVPWQRCQFHLQQNAGALTTRQEARKTVAAQMRAIFNAPDRTEAERLLKAALTL
WCKEHPKLAEWAETAIPESLTVFDFPAAHRIRLRTTNGLERINRELRRRTRVASIFPNPDSCLRLVSALLAELDDEWMTGKVYLNFNP
Blast result :
Comments
The right IR of IS406 contains a -35 promoter box. IS406 increases foreign gene expression, even in heterologous hosts.
References
1] Wood, M.S., Lory, C., and Lessie, T.G. (1990) J. Bacteriol. 172, 1719-1724.
2] Wood, M.S., Byrne, A.M., and Lessie, T.G. (1991) Gene, 105, 101-105.
2] Wood, M.S., Byrne, A.M., and Lessie, T.G. (1991) Gene, 105, 101-105.