IS402
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
Y | Burkholderia cepacia | Burkholderia cepacia ATCC17616 plasmid pTGL6 |
DNA section
IS Length : 914 bp
Ends
IR Length : 16/17
IRL : GAGACGGTTTCAAAAAGGCCCCTGGGGGCTGTTAACTTTCGCGGTGAGCT
IRR : GAGACGGTTTCATAAAGACTGATCGGCCCGCCGAAGGGTATTCCAGCAGA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CGTGATACGC | TTA | TTTTTATAGG | 3 |
DNA sequence
GAGACGGTTTCAAAAAGGCCCCGTGGGGCTGTTAACTTTCGCGGTGAGCTGTTAACCTCAGGCATCGGAACAACCGAGACTGAGGAGATGGCCAAGCCGA
TCATCGACGATGAATTGTGGACACTGATCGAGCCGTTACTGCCGCCACCCAAGCCGCGGCGCGAGAAGAACCCGGGCCGCCTGCCTGTTTCGAATCGCGC
CGCGCTGACCGGCATCCTGTTCGTTCTCAAGACCGGACTACGCTGGCGCGACCTGCCGGCCGAGATGGGATGCGGCTCGGGCGTGACATGTTGGCGCCGG
CTGCGCGATTGGCAAGCAGCCGGTGTCTGGGATCGCTTGCACGAGCTACTGCTCGCAAAGCTGCGCGCAGCGGACCAGATCGACTTCTCACGAGCCGCCG
TCGATTCATCATCGATTCGCGCCGTTGGGGCAGGCCAAAAACTGGGCCAAACCCCACCGATCGCGCGCGACCCGGTTCCAAGCACCACATCGTCACCGAC
GCCAACGGTACGCCGCTCGCCGCGATCCTGACCGGCGCGAACGTCAACGACGTCACGCAATTGCTGCCGCTGATCGACGCGATTCCGCCGATCCGCGGAT
TGCGTGGCCACCCATTGCAGCGGCCGCGTGTGGTCTACGCGGATCGCGGTTACGACTCCGAGCGACATCGGCGCGCGTTGCGCGATCGCGGTATCGAGCC
AGTGATCGCCAAGCGCCGTACCGAACATGGCAGCGGCCTTGGCAAATATCGCTGGGTCGTCGAACGCACGCATGCCTGGCTGCATCACTTCCGTCGTCTC
CGTATTCGTTTCGAGCGCCGTGCAGACATTCACGGCGCGTTCCTCAAACTCGGTTGCTGTCTGATCTGCTGGAATACCCTTCGGCGGGCCGATCAGTCTT
TATGAAACCGTCTC
TCATCGACGATGAATTGTGGACACTGATCGAGCCGTTACTGCCGCCACCCAAGCCGCGGCGCGAGAAGAACCCGGGCCGCCTGCCTGTTTCGAATCGCGC
CGCGCTGACCGGCATCCTGTTCGTTCTCAAGACCGGACTACGCTGGCGCGACCTGCCGGCCGAGATGGGATGCGGCTCGGGCGTGACATGTTGGCGCCGG
CTGCGCGATTGGCAAGCAGCCGGTGTCTGGGATCGCTTGCACGAGCTACTGCTCGCAAAGCTGCGCGCAGCGGACCAGATCGACTTCTCACGAGCCGCCG
TCGATTCATCATCGATTCGCGCCGTTGGGGCAGGCCAAAAACTGGGCCAAACCCCACCGATCGCGCGCGACCCGGTTCCAAGCACCACATCGTCACCGAC
GCCAACGGTACGCCGCTCGCCGCGATCCTGACCGGCGCGAACGTCAACGACGTCACGCAATTGCTGCCGCTGATCGACGCGATTCCGCCGATCCGCGGAT
TGCGTGGCCACCCATTGCAGCGGCCGCGTGTGGTCTACGCGGATCGCGGTTACGACTCCGAGCGACATCGGCGCGCGTTGCGCGATCGCGGTATCGAGCC
AGTGATCGCCAAGCGCCGTACCGAACATGGCAGCGGCCTTGGCAAATATCGCTGGGTCGTCGAACGCACGCATGCCTGGCTGCATCACTTCCGTCGTCTC
CGTATTCGTTTCGAGCGCCGTGCAGACATTCACGGCGCGTTCCTCAAACTCGGTTGCTGTCTGATCTGCTGGAATACCCTTCGGCGGGCCGATCAGTCTT
TATGAAACCGTCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
444 bp | 147 aa | 88 | 531 | + | No |
Description : First part of the transposase
ORF sequence :
MAKPIIDDELWTLIEPLLPPPKPRREKNPGRLPVSNRAALTGILFVLKTGLRWRDLPAEMGCGSGVTCWRRLRDWQAAGVWDRLHELLLAKLRAADQIDF
SRAAVDSSSIRAVGAGQKLGQTPPIARDPVPSTTSSPTPTVRRSPRS
SRAAVDSSSIRAVGAGQKLGQTPPIARDPVPSTTSSPTPTVRRSPRS
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
618 bp | 205 aa | 288 | 905 | + | No |
Description : Second part of the transposase
ORF sequence :
MLAPAARLASSRCLGSLARATARKAARSGPDRLLTSRRRFIIDSRRWGRPKTGPNPTDRARPGSKHHIVTDANGTPLAAILTGANVNDVTQLLPLIDAIP
PIRGLRGHPLQRPRVVYADRGYDSERHRRALRDRGIEPVIAKRRTEHGSGLGKYRWVVERTHAWLHHFRRLRIRFERRADIHGAFLKLGCCLICWNTLRR
ADQSL
PIRGLRGHPLQRPRVVYADRGYDSERHRRALRDRGIEPVIAKRRTEHGSGLGKYRWVVERTHAWLHHFRRLRIRFERRADIHGAFLKLGCCLICWNTLRR
ADQSL
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
818 bp | 273 aa | 88 | 905 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKPIIDDELWTLIEPLLPPPKPRREKNPGRLPVSNRAALTGILFVLKTGLRWRDLPAEMGCGSGVTCWRRLRDWQAAGVWDRLHELLLAKLRAADQIDF
SRAAVDSSSIRAVGAGQKKTGPNPTDRARPGSKHHIVTDANGTPLAAILTGANVNDVTQLLPLIDAIPPIRGLRGHPLQRPRVVYADRGYDSERHRRALR
DRGIEPVIAKRRTEHGSGLGKYRWVVERTHAWLHHFRRLRIRFERRADIHGAFLKLGCCLICWNTLRRADQSL
SRAAVDSSSIRAVGAGQKKTGPNPTDRARPGSKHHIVTDANGTPLAAILTGANVNDVTQLLPLIDAIPPIRGLRGHPLQRPRVVYADRGYDSERHRRALR
DRGIEPVIAKRRTEHGSGLGKYRWVVERTHAWLHHFRRLRIRFERRADIHGAFLKLGCCLICWNTLRRADQSL
Blast result :
Comments
Promotes replicon fusion. IS402 insertion (in both orientations) increases foreign gene expression. IS402 transposase is a "fusion" between two ORFs, (88-441) and (438-905), as suggested by comparison with other IS5 family members. At position 436, the sequence 5'-CAAAAACTGGGCC-3' is a potential site for a -1 frameshifting between both ORFs.
IS402 is 99% aa similar to ISBcen20 and 85% aa similar to ISBugl2.
File updated: July 11 2012.
IS402 is 99% aa similar to ISBcen20 and 85% aa similar to ISBugl2.
File updated: July 11 2012.
References
1] Barsomian, G., and Lessie, T.G. (1986) Mol. Gen. Genet. 204, 273-280.
2] Scordilis, G.E., Ree, H., and Lessie, T.G. (1987) J. Bacteriol. 169, 8-13.
3] Gaffney, T.D., and Lessie, T.G. (1987) J. Bacteriol. 169, 224-230.
4] Ferrante, A.A., and Lessie, T.G. (1991) Gene 102, 143-144.
2] Scordilis, G.E., Ree, H., and Lessie, T.G. (1987) J. Bacteriol. 169, 8-13.
3] Gaffney, T.D., and Lessie, T.G. (1987) J. Bacteriol. 169, 224-230.
4] Ferrante, A.A., and Lessie, T.G. (1991) Gene 102, 143-144.