IS232

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) IR2, IR2150, IS232A, IS232B, IS232C
Accession numberTranspositionOriginHost
M38370 Y Bacillus thuringiensis
Bacillus thuringiensis subsp. Tolworthi
Bacillus thuringiensis subsp. thuringiensis HD2 plasmid p81.87kb
Bacillus thuringiensis subsp. morrisoni
Bacillus thuringiensis subsp. thuringiensis berliner 1715 plasmid p65kb
Bacillus thuringiensis subsp. aizawai 7.29 plasmid pBT45
Bacillus thuringiensis subsp. darmstadiensis
Bacillus thuringiensis subsp. galleriae
Bacillus thuringiensis subsp. kurstaki HD1 plasmid p66kb
Bacillus thuringiensis subsp. kurstaki HD1-Dipel
Bacillus thuringiensis subsp. kurstaki HD73 plasmid p75kb
Bacillus thuringiensis subsp. kurstaki HD244
Bacillus thuringiensis subsp. sotto
Bacillus thuringiensis subsp. thuringiensis F
Bacillus thuringiensis subsp. thuringiensis HD120
Bacillus thuringiensis subsp. thuringiensis HD290
DNA section
IS Length : 2184 bp

Ends


IR Length : 48/67

IRL : GTATAAATGCTAACTTAAATATGTACATTAACGCTTGAATAAATATGTAC
IRR : GTGTAAATGTCAAGATAAACATGTACATTTTCGCTTGTTTAAGCATGTAC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GTATAAATGCTAACTTAAATATGTACATTAACGCTTGAATAAATATGTACATTTTCAATTGGATTCTCCATACTGACTCTGAGGTGGTTAGTATGTATAT
TAAGCTAGATATTCAAACAGAATTTGAGGTTAAAAGTCTTTCAGACTTACCAAATTTTAAAAAACTAATGGGGAACTTAAAAATGAAGATAAATAAAAGT
CAATTAGCCAGAGAATTGAATGTGGATCGGCGTACCATAGATAAGTATTTGAATGGTTTTACACCAAAAGGGACAAAAAATAAAACATCGAAAATCGATA
CATATTATGAAGTGATTGCAGCTCTTTTATCTAGTGATTCTAAACAAATCTTCTACTACAAACGAGTGTTATGGCAGTATCTAACAGACAATCACGGTTT
AAAATGTTCACAGTCTGCATTTCGTGCTTATATTAATAGAAAGCCTGAATTTAGAACATATTTTGATGAGGGGAAGCGTATTTTATCAGGTCATTCAGTG
GGTGTCCGTTATGAGACACCTCCAGGAGAACAAGCTCAATTAGATTGGAAAGAAAGCATACGATTTGAAACCAAAAGTGGCGAAATCGTATATGTGAATG
TAGCTGTACTTTTATTGTCCTACTCGAGATTTAAAGTTTTTCATTTGAATATTTCAAAATCACAAAGTGTTTTAATGTCATTTATGACAGAGGCATTTGA
AATGTTTGGTGGTGTACCGAAGGTAATTGTCACGGATAATATGAAGACGGTAATGGATGAAGCTCGAACAGAACACTTTACAGGAACGATTAACAATAAG
TTTGCCCAATTCGCTCAAGATTTTGGATTTAAGGTACAACCTTGTATCGCAGGGCGACCAAATACCAAAGGGAAAGTAGAAGCGCCAATGAAACTTCTAG
ACGAAATTCATACTTATCAAGGAAGATTCACTTTTGAAGAATTACATGAATTTGTGCAAAAATTATGTGCAAGAATTAATCAAACATTTCATCAAGGGAC
TGGTAAGATTCCAGTGTTTGCCCTAAAACAGGAAAAGAATCTCCTACAACCACTCCCGAAGAGCGCGATAAGAGATTCCTATATGATTAAGCATAAACTT
GTAAAAGTTAATACATCAGGCATGATATCTTACAAATCGAATCAATACTCAGTTCCAGCTGAATATCAAGGTAAAACCGTCGGTTTACAAGTATATGATA
ATCAAATATATGTTTATCATAACATGAAGTTAATTGTACAACATAAAATCAGCCAATCTAAGCTCAATTATAAAGAAGAACATTATAAAAAAGCATTGGC
TAAGTCACTACCTAAATATCCGAACATTGACAATTTGGCGAAACAAAATTTATCAGTAATTGGTGAGGTATATAGAAATGAAGAATAGCTATCAACAATT
AACAACAAACCTAGAGTATTTAAAATTAAAACAAATGGCACAACATTTAGGTGACGTAGTCGATTTTAGCATTAATAATGAATTATCCTTCGTAGAGACA
CTTGTTAAACTGACAAACTATGAGATTGATGTACGAGAACAAAATATGATTCATTCTATGGTGAAAATGGGCGCATTTCCTCATAGAAAGGAGGTTGATG
AGTTTGATTTCGAATTCCAGCCGAGTATTAATAAACAACAAATCTTAGATTTTATTTCTCTACGTTTCTTAGAGCAACAAGAAAACATAGTATTTTTAGG
ACCTAGTGGTGTTGGTAAGACCCATTTGGCCACGTCTATTGGTATAGCAGCAGCTAAAAAGCGAACAAGTACTTATTTTATTAAATGTCATGATTTACTT
CAAAATTTAAAACGTGCCAAGATTGAGAATCGCCTAGAATCTCGTTTAAAGCACTATACAAAATACAAATTACTTATTATTGATGAAATTGGGTACTTGC
CTATTGATCCGGAGGATGCAAAATTATTCTTTCAATTAATCGATATGCGTTATGAAAAGCGTAGTACCATCCTAACGACCAATATCAACTTCAAGTCTTG
GGACGAAGTATTCCAGGACCCTAAACTCGCCAATGCCATACTAGATCGTGTCTTACATCATGCCACGGTGGTCAGTATTGTAGGACAATCCTATCGAATT
AAAGATCATTTTAGCAAAGAAAATGATTGATTTTGTACATGCTTAAACAAGCGAAAATGTACATGTTTATCTTGACATTTACAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1296 bp431 aa931388+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MYIKLDIQTEFEVKSLSDLPNFKKLMGNLKMKINKSQLARELNVDRRTIDKYLNGFTPKGTKNKTSKIDTYYEVIAALLSSDSKQIFYYKRVLWQYLTDN
HGLKCSQSAFRAYINRKPEFRTYFDEGKRILSGHSVGVRYETPPGEQAQLDWKESIRFETKSGEIVYVNVAVLLLSYSRFKVFHLNISKSQSVLMSFMTE
AFEMFGGVPKVIVTDNMKTVMDEARTEHFTGTINNKFAQFAQDFGFKVQPCIAGRPNTKGKVEAPMKLLDEIHTYQGRFTFEELHEFVQKLCARINQTFH
QGTGKIPVFALKQEKNLLQPLPKSAIRDSYMIKHKLVKVNTSGMISYKSNQYSVPAEYQGKTVGLQVYDNQIYVYHNMKLIVQHKISQSKLNYKEEHYKK
ALAKSLPKYPNIDNLAKQNLSVIGEVYRNEE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
753 bp250 aa13782130+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MKNSYQQLTTNLEYLKLKQMAQHLGDVVDFSINNELSFVETLVKLTNYEIDVREQNMIHSMVKMGAFPHRKEVDEFDFEFQPSINKQQILDFISLRFLEQ
QENIVFLGPSGVGKTHLATSIGIAAAKKRTSTYFIKCHDLLQNLKRAKIENRLESRLKHYTKYKLLIIDEIGYLPIDPEDAKLFFQLIDMRYEKRSTILT
TNINFKSWDEVFQDPKLANAILDRVLHHATVVSIVGQSYRIKDHFSKEND

 

Blast result :
Comments
IS232A displays long IR (L: 1-67 and R: 2118-2184/CS) that contains internal DR of 22 bp long. These DR are present three times at the left end and twice at the right end. The two ORF overlap by 11 bp (3 aa).
L: GTATAAATGCTAACTTAAATATGTACATTAACGCTTGAATAAATATGTACATTTTCAATTGGATTCT
|| |||||| || |||| ||||||||| |||||| ||| ||||||| ||||| ||||
R: GTGTAAATGTCAAGATAAACATGTACATTTTCGCTTGTTTAAGCATGTACAAAATCAATCATTTTCT
L1: TAAATATGTACATTAACGCTTG (16-37)
R1: TAAACATGTACATTTTCGCTTG (2148-2169) CS
L2: TAAATATGTACATTTTCAATTGGATTCT (40-67)
R2: TAAGCATGTACAAAATCAATCATTTTCT (2118-2145) CS
L3: TTAGTATGTATATTAAGCTAGATATTCA (88-115), ATG underlined.
This situation is similar to that found in IS1326:
L1: GAGTTGCATCTAAAATTGACCC (5-26)
L2: GATTTGCGTCGAAATTTGACCC (39-60)
L3: GATATTGAGCGCAATTCGACGC (122-143)
R1: GATTTGCATTGAATTTTGACCC (2421-2442) CS
R2: GATTTGCACCCAAATTTGACCC (2445-2466) CS

The 572-bp DNA sequence of IS232B from file M77344 is identical to that of IS232A, although differences must exist between the two ISs (restriction maps different).

July 13 2012 : the file IS232 replace the IS232A, IS232B and IS232C files ; IS232B and IS232C were partial sequences.
References