ISfinder Mirror Site

IS231V

Family IS4
Group IS231

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
M86926	ND	Bacillus thuringiensis	Bacillus cereus BcNi plasmid p360kb Bacillus thuringiensis subsp. israelensis plasmid p112kb Bacillus cereus ATCC11778 plasmid p230kb

DNA section

IS Length : 1964 bp

Ends

IR Length : 21/22

IRL : CATCGCCATCAAGCTAAGGAAAAGGAGATACCCCCAAACCGAATAAAATA
IRR : CATCGCCATCAAGCTAACGAAATTGAACTTACAAAAATACGTATAAATTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

CATCGCCATCAAGCTAAGGAAAAGGAGATACCCCCAAACCGAATAAAATAGGGGTATCTCTTTTTATATATCTAACTCTATTTCTTTGCTCATGCAACAG
AACTTATATTTCTTTCCACTGTTACAAGCACATGTATCATATATATCCATTGACGTGTCCGTCCTTTCATCGCTTTTTTCAAATCTGTTAGATCTTCATT
TAATTCTAAACTAATGATGTAATATAAATCATACTCATCACAAATTGCTGCTATTTTTTCTGCTCGTGCTCTTGTTCCTACTTTTATCATAATCGGATTA
TTTTCTGAACCTAATAGACACATAAGCTTTCACCTCTTTTCTTTTCATCTTCATTTTATAAAATAAAGATAAAAAATGGAGGGGAATCCATGAAAAAATC
ATCTCATTTTAAAGACTTTCGTTTACTTGTAAAAGAATTACAGCGTGTATTTTCATCACACTTTTTAACACATTTGGGGAAAGAAACACAGTTTGTTCAA
CGTACCAGTAAATTTGGAGCACAAGATTTAGCTGCTTTATGCATAGGAATGGGCCAAGATATTGCAAGTTATTCTTTGACAAGACTATGTGGTGTTTTAG
AATCAGAAACAGGTGTCCTTATTAGTCCGGAAGGACTAAATGTACGATTAAACGAAAAAGCAGTAGAATTTTTACGTTCGCTCTTCTCGCAACTCTTACA
AAAACAATTATTAGCCACTATATCTCTACCTTCTTCTTTTTCAGAATACTTTCGGCGTATTCGTATTTTAGATGCCACTACATTTCAAATTTCAGATCAA
CTCGCAGCCGTTTATCCTGGTTCAGGCGGAAGTGGAAAAGCTTCTGGAGTGAAAATACAATTAGAATATGATTTACTGAGTGGTCAATTCTTACATGTTG
CATTGGGACCTGCGAAAGAAAATGATGTGAATTACGGCAAAGAAGTTCAACCTACCGTTGATTTACAAGGTCTTTGTATACGGGATTTAGGTTATTTTAG
TTTGATCGATCTAGATGGCATCCAACAAAAAGGAGCCTACTATTTATCTCGGCTTAAAATGAATACCAAACTGTTTCAAAAAATGAAGAGGCCCCTATTT
TTAAAAATGGAGCAAGTAAGAAAAATATCAATATACAATGATTGATTTAGAAACGATTATGGAACAGTTACAACCAGGGGAATTATATGAAATTCCTGTT
GTCTATGTTGGGCGTGACTATCTACTTCCAGTAAGAGCTGTAATGTATAGGCTTACTCCTGATCAGGAAACACAACGTAGAAAAGATCGAGCATACAAAG
AAAAAAAGAAAAACATAACGTTTAGTGATCGAACAAAAAAATTACAAGGCATCAATGTATACATTACGAATATTCCATTGGAATATGTTTCTAAAGAAGC
TATCCATGAGTTCTACTCTCTTCGTTGGCAAATAGAAATTATCTTTAAAACATGGAAATCTATTTTTCGCATTCATTCCAATACAAATGTGAAGAAAGAA
CGATTAGAATGTCATATCTATGGAAAATTAATTGCTCTTTTACTTTCTTCTACTGTGATGTTCCAAATGAGACAAGTATTGCTGATAAAAAAACAAAAAG
AATTAAGTGAATGGAAAGCTATGTATATGATTCATGATTATTTCCGCGTATTATATCGACAAATACAAGAACAATCGAAGCAACTAATAACAAGTTTTCT
TCGTTTATTCCACTTACTTGATAAAAATGGACGAAAATCACATCGTTATCGAAAAAAAACTGTATTTGATATTTTAGGTACTGCATACGAGCAGTATATA
GAAAAATAAATCCGCTTAAAAAAATTGTAGCCCGCTAGGGTTATTTCGTATGCACTTTTTTATAAAAAATTTTAATATTGGGTAAAAGATACCCAATATT
CCATAGTCGGAGAGGAATTTATACGTATTTTTGTAAGTTCAATTTCGTTAGCTTGATGGCGATG

Recoding section

Recoding by frameshift
Frame +1
Type unknow
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
757 bp	244 aa	389	1145	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
690 bp	229 aa	1120	1809	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

EKYQYTMIDLETIMEQLQPGELYEIPVVYVGRDYLLPVRAVMYRLTPDQETQRRKDRAYKEKKKNITFSDRTKKLQGINVYITNIPLEYVSKEAIHEFYS
LRWQIEIIFKTWKSIFRIHSNTNVKKERLECHIYGKLIALLLSSTVMFQMRQVLLIKKQKELSEWKAMYMIHDYFRVLYRQIQEQSKQLITSFLRLFHLL
DKNGRKSHRYRKKTVFDILGTAYEQYIEK

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1420 bp	473 aa	390	1809	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKSSHFKDFRLLVKELQRVFSSHFLTHLGKETQFVQRTSKFGAQDLAALCIGMGQDIASYSLTRLCGVLESETGVLISPEGLNVRLNEKAVEFLRSLFS
QLLQKQLLATISLPSSFSEYFRRIRILDATTFQISDQLAAVYPGSGGSGKASGVKIQLEYDLLSGQFLHVALGPAKENDVNYGKEVQPTVDLQGLCIRDL
GYFSLIDLDGIQQKGAYYLSRLKMNTKLFQKMKRPLFLKMEQVRKKYQYTMIDLETIMEQLQPGELYEIPVVYVGRDYLLPVRAVMYRLTPDQETQRRKD
RAYKEKKKNITFSDRTKKLQGINVYITNIPLEYVSKEAIHEFYSLRWQIEIIFKTWKSIFRIHSNTNVKKERLECHIYGKLIALLLSSTVMFQMRQVLLI
KKQKELSEWKAMYMIHDYFRVLYRQIQEQSKQLITSFLRLFHLLDKNGRKSHRYRKKTVFDILGTAYEQYIEK

Blast result :

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References