IS222
- Family IS3
- Group IS3
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
U00100 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa AK1220 Pseudomonas aeruginosa phage D3c Pseudomonas aeruginosa PAO-1 Pseudomonas aeruginosa PTO13 |
DNA section
IS Length : 1236 bp
Ends
IR Length : 22/25
IRL : TGTAGTGGTCTACTGATCCCGGACACCGATTTAGGCGAGAATCCTCGCCG
IRR : TGTAGTGGTCAACCCATCCCGGACAGTGGGTTGAGTTTTTCTTCGGCCAC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|
DNA sequence
TGTAGTGGTCTACTGATCCCGGACACCGATTTAGGCGAGAATCCTCGCCGTGAAAGAGGTGTCTGATGAGCAAGCAACGACGTACGTTTTCCGCCGAGTT
CAAACGAGAGGCCGCGGCCCTGGTGTTGGACCAAGGCTACAGCCATATCGACGCCTGCCGTTCGCTGGGGGTGGTGGATTCGGCCTTGCGCCGTTGGGTG
AAACAGCTCCAGGCGGAACGCCAAGGCGTGACCCCGAAGAGCAAGGCGCTGACGCCCGAACAGCAGAAGATCCAGGAACTGGAAGCCCGGATCAACCGGC
TGGAGCGGGAGAAAGCGATCCTAAAAAAGGCTACCGCTCTCTTGATGTCGGACGAACTCGATCGTACGCGCTGATAGACCAGTTGAGTGAGCAGGAATCG
GTGGAAGTGGTCTGTTCAGCTTTCGATGTGGCGCGGTCTTGCTACTACGTCCACCGTCTTCGACGTCGTCGGGTCGATGCTCGTCGCATTGTGCTACGCA
GCCAGGTCAACCAGTTGTTCAGCCAGAGTCGGGGCTCGGCCGGCAGCCGCAGCATCCTGGGCATGCTGCGCGAAGAGGGCGTGTCCATCGGCCGTTTCCG
AGTGCGTAGGTTGATGCGTGAGCTGGGCCTGGTCAGCAAGCAACCGGGCTCGCACGCCTACAAACAGGCCACGGTTGAGCGGCCGGATATCCCGAATCGG
CTGAACCGCGAATTCGCGACCGAGCATCCCAATCAGGTGTGGTGTGGCGACATCACCTACGTCTGGGCGCAAGGCCGTTGGCACTACCTGGCCGCGGTGC
TGGATCTGCATACCCGGCGGGTGATCGGCTGGGCGTTCTCGGCCAAGCCGGATGCCGAACTGGTGATCAAGGCCCTGGACATGGCCTACGAACAGCGCGG
CAAGCCACAGCAGGTGCTGTTCCATTCAGACCAGGGCAGCCAGTACGCCAGCCGCCTGTTTCGGCAACGGCTCTGGCGCTATCGGATGCAGCAGAGCATG
AGTCGCCGAGGGAATTGCTGGGATAACTCGCCGATGGAGCGCCTGTTCCGCAGTCTGAAGTCGGAGTGGGTCCCGTCAACGGGTTACCTGACGGCGCAGG
AGGCCCAACGGGACATCAGTCATTACTTGATGCACCGCTACAACTGGATCAGGCCGCATCAATTCAACGACGGGTTACCACCTGCGGTGGCCGAAGAAAA
ACTCAACCCACTGTCCGGGATGGGTTGACCACTACA
CAAACGAGAGGCCGCGGCCCTGGTGTTGGACCAAGGCTACAGCCATATCGACGCCTGCCGTTCGCTGGGGGTGGTGGATTCGGCCTTGCGCCGTTGGGTG
AAACAGCTCCAGGCGGAACGCCAAGGCGTGACCCCGAAGAGCAAGGCGCTGACGCCCGAACAGCAGAAGATCCAGGAACTGGAAGCCCGGATCAACCGGC
TGGAGCGGGAGAAAGCGATCCTAAAAAAGGCTACCGCTCTCTTGATGTCGGACGAACTCGATCGTACGCGCTGATAGACCAGTTGAGTGAGCAGGAATCG
GTGGAAGTGGTCTGTTCAGCTTTCGATGTGGCGCGGTCTTGCTACTACGTCCACCGTCTTCGACGTCGTCGGGTCGATGCTCGTCGCATTGTGCTACGCA
GCCAGGTCAACCAGTTGTTCAGCCAGAGTCGGGGCTCGGCCGGCAGCCGCAGCATCCTGGGCATGCTGCGCGAAGAGGGCGTGTCCATCGGCCGTTTCCG
AGTGCGTAGGTTGATGCGTGAGCTGGGCCTGGTCAGCAAGCAACCGGGCTCGCACGCCTACAAACAGGCCACGGTTGAGCGGCCGGATATCCCGAATCGG
CTGAACCGCGAATTCGCGACCGAGCATCCCAATCAGGTGTGGTGTGGCGACATCACCTACGTCTGGGCGCAAGGCCGTTGGCACTACCTGGCCGCGGTGC
TGGATCTGCATACCCGGCGGGTGATCGGCTGGGCGTTCTCGGCCAAGCCGGATGCCGAACTGGTGATCAAGGCCCTGGACATGGCCTACGAACAGCGCGG
CAAGCCACAGCAGGTGCTGTTCCATTCAGACCAGGGCAGCCAGTACGCCAGCCGCCTGTTTCGGCAACGGCTCTGGCGCTATCGGATGCAGCAGAGCATG
AGTCGCCGAGGGAATTGCTGGGATAACTCGCCGATGGAGCGCCTGTTCCGCAGTCTGAAGTCGGAGTGGGTCCCGTCAACGGGTTACCTGACGGCGCAGG
AGGCCCAACGGGACATCAGTCATTACTTGATGCACCGCTACAACTGGATCAGGCCGCATCAATTCAACGACGGGTTACCACCTGCGGTGGCCGAAGAAAA
ACTCAACCCACTGTCCGGGATGGGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
309 bp | 102 aa | 66 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MSKQRRTFSAEFKREAAALVLDQGYSHIDACRSLGVVDSALRRWVKQLQAERQGVTPKSKALTPEQQKIQELEARINRLEREKAILKKATALLMSDELDR
TR
TR
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1194 bp | 397 aa | 35 | 1228 | + | No |
Description : Second part of the transposase
ORF sequence :
ARILAVKEVSDEQATTYVFRRVQTRGRGPGVGPRLQPYRRLPFAGGGGFGLAPLGETAPGGTPRRDPEEQGADARTAEDPGTGSPDQPAGAGESDPKKGY
RSLDVGRTRSYALIDQLSEQESVEVVCSAFDVARSCYYVHRLRRRRVDARRIVLRSQVNQLFSQSRGSAGSRSILGMLREEGVSIGRFRVRRLMRELGLV
SKQPGSHAYKQATVERPDIPNRLNREFATEHPNQVWCGDITYVWAQGRWHYLAAVLDLHTRRVIGWAFSAKPDAELVIKALDMAYEQRGKPQQVLFHSDQ
GSQYASRLFRQRLWRYRMQQSMSRRGNCWDNSPMERLFRSLKSEWVPSTGYLTAQEAQRDISHYLMHRYNWIRPHQFNDGLPPAVAEEKLNPLSGMG
RSLDVGRTRSYALIDQLSEQESVEVVCSAFDVARSCYYVHRLRRRRVDARRIVLRSQVNQLFSQSRGSAGSRSILGMLREEGVSIGRFRVRRLMRELGLV
SKQPGSHAYKQATVERPDIPNRLNREFATEHPNQVWCGDITYVWAQGRWHYLAAVLDLHTRRVIGWAFSAKPDAELVIKALDMAYEQRGKPQQVLFHSDQ
GSQYASRLFRQRLWRYRMQQSMSRRGNCWDNSPMERLFRSLKSEWVPSTGYLTAQEAQRDISHYLMHRYNWIRPHQFNDGLPPAVAEEKLNPLSGMG
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1163 bp | 387 aa | 66 | 1228 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKQRRTFSAEFKREAAALVLDQGYSHIDACRSLGVVDSALRRWVKQLQAERQGVTPKSKALTPEQQKIQELEARINRLEREKAILKKGYRSLDVGRTRS
YALIDQLSEQESVEVVCSAFDVARSCYYVHRLRRRRVDARRIVLRSQVNQLFSQSRGSAGSRSILGMLREEGVSIGRFRVRRLMRELGLVSKQPGSHAYK
QATVERPDIPNRLNREFATEHPNQVWCGDITYVWAQGRWHYLAAVLDLHTRRVIGWAFSAKPDAELVIKALDMAYEQRGKPQQVLFHSDQGSQYASRLFR
QRLWRYRMQQSMSRRGNCWDNSPMERLFRSLKSEWVPSTGYLTAQEAQRDISHYLMHRYNWIRPHQFNDGLPPAVAEEKLNPLSGMG
YALIDQLSEQESVEVVCSAFDVARSCYYVHRLRRRRVDARRIVLRSQVNQLFSQSRGSAGSRSILGMLREEGVSIGRFRVRRLMRELGLVSKQPGSHAYK
QATVERPDIPNRLNREFATEHPNQVWCGDITYVWAQGRWHYLAAVLDLHTRRVIGWAFSAKPDAELVIKALDMAYEQRGKPQQVLFHSDQGSQYASRLFR
QRLWRYRMQQSMSRRGNCWDNSPMERLFRSLKSEWVPSTGYLTAQEAQRDISHYLMHRYNWIRPHQFNDGLPPAVAEEKLNPLSGMG
Blast result :
Comments
IS222 is inserted within the first nine codon of the phage D3 repressor gene (c1). IS222 ORFA and ORFAB are 57 and 54 % identical to those of IS911, their closest relative. Note that ORF2 spans the entire IS222, from position 48 to 1223.
Modified the 04/11/2011 : the sequence was extended of two bp on each end with a TG (left) and CA (rigth). These data were obtain after comparison with the complete sequenced genomes.
Modified the 04/11/2011 : the sequence was extended of two bp on each end with a TG (left) and CA (rigth). These data were obtain after comparison with the complete sequenced genomes.
References
1] Gertman, E., White, B.N., Berry, D., and Kropinski, A.M. (1986) J. Bacteriol. 166, 1134-1136.
2] Kropinski, A.M., Farinha, M.A., and Jansons, I. (1994) Plasmid 31, 222-228.
2] Kropinski, A.M., Farinha, M.A., and Jansons, I. (1994) Plasmid 31, 222-228.