IS2112
- Family IS110
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF060871 | ND | Rhodococcus rhodochrous | Rhodococcus rhodochrous NCIMB13064 |
DNA section
IS Length : 1415 bp
Ends
Left end : TCCGTAAGTTGTTAGTTGGTCCGGAAGTGCTGGGTGTGGCTGATGGTGTCTTGCCTTGTGTGGCTCGAAACGAGTGGCCGCCCAGCGCTTCGGGACGCCC
Right end : GCGTCGACAAGTGAACGTGATCTGGGCATTGTTGCGCGACGACCGGCTGTTCATCGTTGATGCACCCGCTCTTCGAACTGCTTGATTTCCTCATTGAAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TCCGTAAGTTGTTAGTTGGTCCGGAAGTGCTGGGTGTGGCTGATGGTGTCTTGCCTTGTGTGGCTCGAAACGAGTGGCCGCCCAGCGCTTCGGGACGCCC
TGGCTGCTGATTGAAAAATGTGCTTCGAGCACTGTTTACGGAGATGACGGTGGGGTGTTCCTCGGGCCTGACACGAGTCGGCGACATGGAGGAGCGAATG
AGAGAAACTCACACCCACGCATGGGTCGGAATCGACGCAGGCAAGGGCCATCATTGGGCAGCAGTGGTCGACGAAGCAGGGTTGATCGTGTGGTCGCACA
AGATCATCAACGACGAGACCGCGATACTGCACGCGCTGAGCGAGATTCTGGCCTTGGCCGACGAACTCCATTGGGCGGTAGATATTTCCGGAACCTCCTC
GACGTTGCTGCTCGCTCTTCTCGCCGCACACGACCAGAGTGCGGTGTACGTCCCTGGTCGCACCGTCAACAGGATGTCCGGCGCGTATCGAGGGGAGGCG
AAAACCGATGCCCGCGATGCCTATGTGATCGCGGAAACTGCTCGACACCGACATGATCTGTCTGTGTTGATCCCACCGGCTCAGTTGGCCGCGGAACTCG
CGCTGTTGACCTCGCATCGGACCGATCTCGTCGCTGACCGAGTCCGGCTGATCAACCGTCTGCGTGACACTCTCACCGGTGTCTTTCCTGCGCTCGAGCG
GGCTTTCGACTTCGGGCGACACAAGGGCGCGTTGGTGTTGTTGACCGGTTTTCAGACCCCGCGTGCCATTCGGCGACGCGGACGCACTCGCTTGAGCTTG
TGGCTTGGTAAACGTGGGGTGCGCGGGGCGGACGGTGTCGCCGAGATTGCGTGGGAGGCGGCGTCTGCGCAGAATGTGGTCCTGCCTGGTGAGGCTGTTG
CGGCGCAGTTGGTGGCCGATATCGCCGACCGAGTCCTCGCAGTGGACGTCCAGCTCGCTCGTATCGACGCGCAGATCGGTGAGACCTTCCGCCGCCATCC
GCAGGCTGCGGTCATCGAATCCATGCCCGGTATCGGTCCGATCCTCGGCGCGGAATTCGTTGTCGCTGCCGGTGATATGTCGGCGTACTCCGATGCCGGA
CACCTTGCCTCGGCAGCCGGTTTGGTTCCGGTCCCCCGCGATTCCGGTCGCCGAACCGGCAACATGCACCGACCCAAACGCTACAGCCGGCGACTGCGGC
GGGTCTTCTACCTCTCCGCTCAAACCTCGATCATCAAAGATGGACCGAATCGCGAGTTCTACCTCCAGAAACGAAGTGAAGGAATGAGGCACGTTCAAGC
GGTTATCGCCTTGGCGCGTCGACAAGTGAACGTGATCTGGGCATTGTTGCGCGACGACCGGCTGTTCATCGTTGATGCACCCGCTCTTCGAACTGCTTGA
TTTCCTCATTGAAAC
TGGCTGCTGATTGAAAAATGTGCTTCGAGCACTGTTTACGGAGATGACGGTGGGGTGTTCCTCGGGCCTGACACGAGTCGGCGACATGGAGGAGCGAATG
AGAGAAACTCACACCCACGCATGGGTCGGAATCGACGCAGGCAAGGGCCATCATTGGGCAGCAGTGGTCGACGAAGCAGGGTTGATCGTGTGGTCGCACA
AGATCATCAACGACGAGACCGCGATACTGCACGCGCTGAGCGAGATTCTGGCCTTGGCCGACGAACTCCATTGGGCGGTAGATATTTCCGGAACCTCCTC
GACGTTGCTGCTCGCTCTTCTCGCCGCACACGACCAGAGTGCGGTGTACGTCCCTGGTCGCACCGTCAACAGGATGTCCGGCGCGTATCGAGGGGAGGCG
AAAACCGATGCCCGCGATGCCTATGTGATCGCGGAAACTGCTCGACACCGACATGATCTGTCTGTGTTGATCCCACCGGCTCAGTTGGCCGCGGAACTCG
CGCTGTTGACCTCGCATCGGACCGATCTCGTCGCTGACCGAGTCCGGCTGATCAACCGTCTGCGTGACACTCTCACCGGTGTCTTTCCTGCGCTCGAGCG
GGCTTTCGACTTCGGGCGACACAAGGGCGCGTTGGTGTTGTTGACCGGTTTTCAGACCCCGCGTGCCATTCGGCGACGCGGACGCACTCGCTTGAGCTTG
TGGCTTGGTAAACGTGGGGTGCGCGGGGCGGACGGTGTCGCCGAGATTGCGTGGGAGGCGGCGTCTGCGCAGAATGTGGTCCTGCCTGGTGAGGCTGTTG
CGGCGCAGTTGGTGGCCGATATCGCCGACCGAGTCCTCGCAGTGGACGTCCAGCTCGCTCGTATCGACGCGCAGATCGGTGAGACCTTCCGCCGCCATCC
GCAGGCTGCGGTCATCGAATCCATGCCCGGTATCGGTCCGATCCTCGGCGCGGAATTCGTTGTCGCTGCCGGTGATATGTCGGCGTACTCCGATGCCGGA
CACCTTGCCTCGGCAGCCGGTTTGGTTCCGGTCCCCCGCGATTCCGGTCGCCGAACCGGCAACATGCACCGACCCAAACGCTACAGCCGGCGACTGCGGC
GGGTCTTCTACCTCTCCGCTCAAACCTCGATCATCAAAGATGGACCGAATCGCGAGTTCTACCTCCAGAAACGAAGTGAAGGAATGAGGCACGTTCAAGC
GGTTATCGCCTTGGCGCGTCGACAAGTGAACGTGATCTGGGCATTGTTGCGCGACGACCGGCTGTTCATCGTTGATGCACCCGCTCTTCGAACTGCTTGA
TTTCCTCATTGAAAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1203 bp | 400 aa | 188 | 1390 | + | No |
Chemistry : DEDD
ORF sequence :
MRETHTHAWVGIDAGKGHHWAAVVDEAGLIVWSHKIINDETAILHALSEILALADELHWAVDISGTSSTLLLALLAAHDQSAVYVPGRTVNRMSGAYRGE
AKTDARDAYVIAETARHRHDLSVLIPPAQLAAELALLTSHRTDLVADRVRLINRLRDTLTGVFPALERAFDFGRHKGALVLLTGFQTPRAIRRRGRTRLS
LWLGKRGVRGADGVAEIAWEAASAQNVVLPGEAVAAQLVADIADRVLAVDVQLARIDAQIGETFRRHPQAAVIESMPGIGPILGAEFVVAAGDMSAYSDA
GHLASAAGLVPVPRDSGRRTGNMHRPKRYSRRLRRVFYLSAQTSIIKDGPNREFYLQKRSEGMRHVQAVIALARRQVNVIWALLRDDRLFIVDAPALRTA
AKTDARDAYVIAETARHRHDLSVLIPPAQLAAELALLTSHRTDLVADRVRLINRLRDTLTGVFPALERAFDFGRHKGALVLLTGFQTPRAIRRRGRTRLS
LWLGKRGVRGADGVAEIAWEAASAQNVVLPGEAVAAQLVADIADRVLAVDVQLARIDAQIGETFRRHPQAAVIESMPGIGPILGAEFVVAAGDMSAYSDA
GHLASAAGLVPVPRDSGRRTGNMHRPKRYSRRLRRVFYLSAQTSIIKDGPNREFYLQKRSEGMRHVQAVIALARRQVNVIWALLRDDRLFIVDAPALRTA
Blast result :
Comments
This element is closely related to IS116 (69% aa identity). A second copy has been found in plasmid pRTL1. IS2112 is involved in genome rearrangements.
References
1] Kulakov, L.A., Poelarends, G.J., Janssen, D.B., and Larkin, M. (1999). Characterization of IS2112, a new insertion sequence from Rhodocuccus, and its realtionship with mobile elements belonging to the IS110 family. Microbiology 145(3), 561-568.