IS1N
- Family IS1
- Group
Isoform Synonym(s) NuXi
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| J01737 | ND | Shigella dysenteriae | Shigella dysenteriae |
DNA section
IS Length : 766 bp
Ends
IR Length : 20/23
IRL : GGTGATGCCTCTAATTAGTTGAATCTGATGTATAATGCGGGCTTTTGAGG
IRR : GGTGATGCTTCCAATTAGTAGAACATGTGTTTTTCAATAAACGTCCCGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTGATGCCTCTAATTAGTTGAATCTGATGTATAATGCGGGCTTTTGAGGTTCTTTCATGGCCAGCGTTAACATTCATTGTCCTCGTTGTCAGTCTGCAC
AGGTCTACCGCCATGGTCAGAACCCTAAAGGCCATGACAGATTTCGCTGCCGTGACTGCCACCGCGTTTTTCAGCTCACTTACACTTATGAGGCCCGTAA
GCCGGGCATTAAAGAGCTGATCACTGAAATGGCCTTCAATGGCGCCGGGGTTCGCGATACCGCCAGGACACTGAAAATTGGCATTAACACCGTCATCCGG
ACTTTAAAAAACTCACGCCAAAGCGAATAACGTCTTCGCCCGTTGCTCATGCTGATGTGGCGCTTATCTGCGAACTTGATGAGCAATGGAGTTTCGTTGG
CAGTAAAGCCCGGCAACACTGGCTCTGGTACGCGTATAACACCAAAACAGGGGGGGTACTGGCCTACACTTTTGGTCCCCGTACCGATGAAACCTGCCGG
GAACTACTGGCACTGCTTACGCCATTCAACATCGGCATGCTCACCAGCGACGACTGGGGCAGCTATGGCCGGGAGGTGCCGAAGGATAAGCATCTGACCG
GAAAAATATTCACCCAACGGATTGAGCGCAATAACCTGACGCTGCGCACCCGCATTAAGCGCTTGGCTCGTAAAACAATCTGCTTCTCGCGTTCAGTAGA
GATCCACGAAAAAGTCATCGGGACGTTTATTGAAAAACACATGTTCTACTAATTGGAAGCATCACC
AGGTCTACCGCCATGGTCAGAACCCTAAAGGCCATGACAGATTTCGCTGCCGTGACTGCCACCGCGTTTTTCAGCTCACTTACACTTATGAGGCCCGTAA
GCCGGGCATTAAAGAGCTGATCACTGAAATGGCCTTCAATGGCGCCGGGGTTCGCGATACCGCCAGGACACTGAAAATTGGCATTAACACCGTCATCCGG
ACTTTAAAAAACTCACGCCAAAGCGAATAACGTCTTCGCCCGTTGCTCATGCTGATGTGGCGCTTATCTGCGAACTTGATGAGCAATGGAGTTTCGTTGG
CAGTAAAGCCCGGCAACACTGGCTCTGGTACGCGTATAACACCAAAACAGGGGGGGTACTGGCCTACACTTTTGGTCCCCGTACCGATGAAACCTGCCGG
GAACTACTGGCACTGCTTACGCCATTCAACATCGGCATGCTCACCAGCGACGACTGGGGCAGCTATGGCCGGGAGGTGCCGAAGGATAAGCATCTGACCG
GAAAAATATTCACCCAACGGATTGAGCGCAATAACCTGACGCTGCGCACCCGCATTAAGCGCTTGGCTCGTAAAACAATCTGCTTCTCGCGTTCAGTAGA
GATCCACGAAAAAGTCATCGGGACGTTTATTGAAAAACACATGTTCTACTAATTGGAAGCATCACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 273 bp | 90 aa | 58 | 330 | + | No |
Description : First part of the transposase
ORF sequence :
MASVNIHCPRCQSAQVYRHGQNPKGHDRFRCRDCHRVFQLTYTYEARKPGIKELITEMAFNGAGVRDTARTLKIGINTVIRTLKNSRQSE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 465 bp | 154 aa | 288 | 752 | + | No |
Description : Second part of the transposase
ORF sequence :
HRHPDFKKLTPKRITSSPVAHADVALICELDEQWSFVGSKARQHWLWYAYNTKTGGVLAYTFGPRTDETCRELLALLTPFNIGMLTSDDWGSYGREVPKD
KHLTGKIFTQRIERNNLTLRTRIKRLARKTICFSRSVEIHEKVIGTFIEKHMFY
KHLTGKIFTQRIERNNLTLRTRIKRLARKTICFSRSVEIHEKVIGTFIEKHMFY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 695 bp | 231 aa | 58 | 752 | + | Yes |
Chemistry : DDE
ORF sequence :
MASVNIHCPRCQSAQVYRHGQNPKGHDRFRCRDCHRVFQLTYTYEARKPGIKELITEMAFNGAGVRDTARTLKIGINTVIRTLKKLTPKRITSSPVAHAD
VALICELDEQWSFVGSKARQHWLWYAYNTKTGGVLAYTFGPRTDETCRELLALLTPFNIGMLTSDDWGSYGREVPKDKHLTGKIFTQRIERNNLTLRTRI
KRLARKTICFSRSVEIHEKVIGTFIEKHMFY
VALICELDEQWSFVGSKARQHWLWYAYNTKTGGVLAYTFGPRTDETCRELLALLTPFNIGMLTSDDWGSYGREVPKDKHLTGKIFTQRIERNNLTLRTRI
KRLARKTICFSRSVEIHEKVIGTFIEKHMFY
Blast result :
Comments
The third ORF is insAB (in silico fusion).
References
1] Ohtsubo, H., Nyman, K., Doroszkkiewicz, W., and Ohtsubo, E. (1981) Nature 292, 640-643.