IS18

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF043676 Y Acinetobacter sp.
Acinetobacter sp. 13 BM2716
DNA section
IS Length : 1074 bp

Ends


IR Length : 20/26

IRL : CGCAGATTCAAATCCCAAGTGCAACACATTTGTAGTCAGTTGAAAAAGTT
IRR : GGCGGATTCAAGCGGCAAGTGCAACAGTATAAAAACCAGCCATAACTTCA

Insertion site


Left flankDirect repeatRight flankDR Length
TGCTTTATATCCTTCATTGATAAAATCTGGATG3
GAAGGTTCTCAAAAAGGTGATGGTGAATACCGG3

DNA sequence

CGCAGATTCAAATCCCAAGTGCAACACATTTGTAGTCAGTTGAAAAAGTTAGGTGTATTCATAGAATCATTAGACTTTTTCAACTCGCAATTGGAAAGCA
CACAATGAAGAAATACACCCAACTTTCTCAAGATGAAAGATACGAAATTTATGCTACGTTGAAAAGTAAAGGCTCAATCGCCTCCCTTGTTCGGGAGTTA
GGACGTTCACGATCAACCATCTACCGTGAATTAAAAAGAAATACTGGGCAACGTGGATATAGAGCTCAACAGGCAGCTAAATTTGCAAGTCAAAGACGGT
ACCGTCCTTCATCATCAATGACAGCATTTGCCTTCGCTTATATTGATTATTTGATTGGTTTGGACTGGTCACCAGAACAAATTTCAGGTGCTTTAACACA
ACGCGGTTGGCTGGATGTACCTTCACATGAGTGGATTTACCAGTACATTTATCAAGATAAATCAAAAGGCGGTAAACTCCATCTACACTTAAGGCATCAG
AAGAAATATCGAAAACGCGGTTACAAAAACACGGATCGTAGGGGTCAAATCATTGATAAAACAAGTATTCACTGCCGAGACCAGGTCATTGATCAACGAC
AACGTTTAGGAGATTTCGAAGGTGACACGGTGATTGGTAAACATCATAAAGGTGCTTTATTAACCCTTGTTGATCGAAAGAGCCTGTATGTACATATTGT
TCATTTAGGATCAACGAGAGCATCCTCTCAAACGATTACTTGTGCGTTAGATCGTTTACGAATGAGCCATGCTTATAGTGTGACATTTGATAATGGTAAA
GAATTTTCCGAACACAAAAGAATTACCGATGCTGGCATAGAGACGTATTTTGCTGATCCTTACAAGTCTATTCAGCGAGCTAGAAATGAAAATACGAATG
GTTTAATCCGTCAATATCTGCCAAAATCATCATCGTTTGATGGCGTGTCAAACGAACAAATAGAGCAGATAGAATTTGCACTCAACCATCGTCCTAGAAA
AACACTAGGTTGGTATACACCGAGTGAAGTTATGGCTGGTTTTTATACTGTTGCACTTGCCGCTTGAATCCGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
963 bp320 aa1051067+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKYTQLSQDERYEIYATLKSKGSIASLVRELGRSRSTIYRELKRNTGQRGYRAQQAAKFASQRRYRPSSSMTAFAFAYIDYLIGLDWSPEQISGALTQR
GWLDVPSHEWIYQYIYQDKSKGGKLHLHLRHQKKYRKRGYKNTDRRGQIIDKTSIHCRDQVIDQRQRLGDFEGDTVIGKHHKGALLTLVDRKSLYVHIVH
LGSTRASSQTITCALDRLRMSHAYSVTFDNGKEFSEHKRITDAGIETYFADPYKSIQRARNENTNGLIRQYLPKSSSFDGVSNEQIEQIEFALNHRPRKT
LGWYTPSEVMAGFYTVALAA

 

Blast result :
Comments
Not present in 10 strains each of Acinetobacter baumanii and Acinetobacter haemolyticus
Insertion of IS18 into a silent aac(6')-Ij gene resulted in its activation possibly by using a -35 (TTGCCG - position 1097 to 1102) in the right end to create a hybrid promoter. PCR generated a fragment in which two ends were abutted and separated by 2bp as in case of IS30.
References
1] Rudant, E., Courvalin, P., and Lambert, T. (1998) Antimicrob. Agents and Chemoth., 42, 2579-2761.