IS1630

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF100324 ND Mycoplasma fermentans
Mycoplasma fermentans II-20/1
Mycoplasma fermentans PG18
DNA section
IS Length : 1423 bp

Ends


IR Length : 44/50

IRL : AATGCTGGATATTTTCCAGCATTGCTACATTGTAATCTGAAACTTTACAC
IRR : AATGCTGGAAAATATCCAGCATTCCTAGATTGTAATCTGTAAGTTTACAC

Insertion site


Left flankDirect repeatRight flankDR Length
TAATCAAAAAAATGCTGGAAAATATCCAGCATTTTTTATTTTA23
AAAAATTAAAAAAGACCATTGTGGTCTTTTTTTACATAC19
TCAATATTAATATCAAGCTTATTATTGAGCTTGATATTTTTTACTT26
AAGAAAAAAGAATATTGCTAATTAAAGCAATATTCTATTTTTAA24
GTATAAAAAGACTTAAATCTTTTTTGGATTTAAGTCTAATTCTTT25
ATAAAAAATAGACCTTAACATTATGTTAAAGTCTATATTATTA23

DNA sequence

AATGCTGGATATTTTCCAGCATTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAGAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCA
CCCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAG
TATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGGGGTTATTTTGCTTTAGATGCA
ATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAAT
ATGATGGCAAATTGTGAACTGTCAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAAT
AAAAAGTGGTTATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTT
GGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTATAGGCAAGAGTA
AAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTAATAAAAATCCATGAAATTTGAATTTGTA
TTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTA
AAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGGGGTTTAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTA
ATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTAGAAATGTA
CGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAATTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAAT
TTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTC
CTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGG
AATGCTGGATATTTTCCAGCATT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1101 bp366 aa1331233+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQKKLSWNERTVLEYLLWATNYSITKIAKILGYSRTTINNEIKNNSTFWGYFALDAIEKTITREKWKNHFKFLNNLNKYKEFSSLFKKEYDGKLWTVKLT
IEKIKNLYPNIDVPSFKTVYNWIKSGYWILNWKGLLRKVYKKGGKREKQSAARRLVGARYVRPFWSRPANINERKEFGHWEIDLIIGKSKGTHCHLLSFT
ERVSRYGFLVKIPNKNPWNLNLYLWNLIWKHKLNVKSITQDNGLEFSTLFFIGYKLKIFIYKADPYASFQRGLNENFNGLVRRFFKKKTNFDDITEEEIL
KVQNEINNRPREIFGYLSALEMYEKLNAENPWNSTGIDKLLYGKQKRNWESNKNRNLFFKKIGKLK

 

Blast result :
Comments
Ends of sequence defined by comparison of an empty site.
The inverted terminal repeat are longer than expected for this family element. A strong palindrome resembling transcription terminators appears to act as a target site in all 7 sequenced cases : AATGCTGGA.
Southern hybridazation indicated at least 7 copies in strain PG18.
The accession number of this IS are AF100324 (IS1630A), AF179373 (IS1630B), AF179374 (IS1630C) , AF179375 (IS1630D) , AF179376 (IS1630E), AF179377 (IS1630F) , AF179378 (IS1630G), Af179379 (IS1630H), AF179380 (IS1630I).
References