IS15DI
- Family IS6
- Group
Isoform IS26Synonym(s) IS15-DeltaI
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M12900 | Y | Salmonella panama | Salmonella ordonez plasmid pIP565 Salmonella panama plasmid pIP111 Salmonella panama LA46 plasmid pIP112 Klebsiella aerogenes K-16R13 plasmid pTH1 Klebsiella pneumoniae 68M plasmid pIP151 Salmonella ordonez BM2000 Acinetobacter baumannii BM2580 Acinetobacter calcoaceticus plasmid pIP1031 Enterobacter cloacae plasmid pIP135-1 Escherichia chloacae Escherichia coli plasmid pIP186 Morganella morganii Providencia mirabilis Providencia stuartii BM2667 plasmid pIP184 Pseudomonas aeruginosa BM2683 |
DNA section
IS Length : 820 bp
Ends
IR Length : 14
IRL : GGCACTGTTGCAAATAGTCGGTGGTGATAAACTTATCATCCCCTTTTGCT
IRR : GGCACTGTTGCAAAGTTAGCGATGAGGCAGCCTTTTGTCTTATTCAAAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNATGGTG | GCGTAAGC | CGTCTTNNNN | 8 |
DNA sequence
GGCACTGTTGCAAATAGTCGGTGGTGATAAACTTATCATCCCCTTTTGCTGATGGAGCTGCACATGAACCCATTCAAAGGCCGGCATTTTCAGCGTGACA
TCATTCTGTGGGCCGTACGCTGGTACTGCAAATACGGCATCAGTTACCGTGAGCTGCAGGAGATGCTGGCTGAACGCGGAGTGAATGTCGATCACTCCAC
GATTTACCGCTGGGTTCAGCGTTATGCGCCTGAAATGGAAAAACGGCTGCGCTGGTACTGGCGTAACCCTTCCGATCTTTGCCCGTGGCACATGGATGAA
ACCTACGTGAAGGTCAATGGCCGCTGGGCGTATCTGTACCGGGCCGTCGACAGCCGGGGCCGCACTGTCGATTTTTATCTCTCCTCCCGTCGTAACAGCA
AAGCTGCATACCGGTTTCTGGGTAAAATCCTCAACAACGTGAAGAAGTGGCAGATCCCACGATTCATCAACACGGATAAAGCGCCCGCCTATGGTCGCGC
GCTTGCTCTGCTCAAACGCGAAGGCCGGTGCCCGTCTGACGTTGAACACCGACAGATTAAGTACCGGAACAACGTGATTGAATGCGATCATGGCAAACTG
AAACGGATAATCAACGCCACGCTGGGATTTAAATCCATGAAGACGGCTTACGCCACCATCAAAGGTATTGAGGTGATGCGTGCACTACGCAAAGGCCAGG
CCTCAGCATTTTATTATGGTGATCCCCTGGGCGAAATGCGCCTGGTAAGCAGAGTTTTTGAAATGTAAGGCCTTTGAATAAGACAAAAGGCTGCCTCATC
GCTAACTTTGCAACAGTGCC
TCATTCTGTGGGCCGTACGCTGGTACTGCAAATACGGCATCAGTTACCGTGAGCTGCAGGAGATGCTGGCTGAACGCGGAGTGAATGTCGATCACTCCAC
GATTTACCGCTGGGTTCAGCGTTATGCGCCTGAAATGGAAAAACGGCTGCGCTGGTACTGGCGTAACCCTTCCGATCTTTGCCCGTGGCACATGGATGAA
ACCTACGTGAAGGTCAATGGCCGCTGGGCGTATCTGTACCGGGCCGTCGACAGCCGGGGCCGCACTGTCGATTTTTATCTCTCCTCCCGTCGTAACAGCA
AAGCTGCATACCGGTTTCTGGGTAAAATCCTCAACAACGTGAAGAAGTGGCAGATCCCACGATTCATCAACACGGATAAAGCGCCCGCCTATGGTCGCGC
GCTTGCTCTGCTCAAACGCGAAGGCCGGTGCCCGTCTGACGTTGAACACCGACAGATTAAGTACCGGAACAACGTGATTGAATGCGATCATGGCAAACTG
AAACGGATAATCAACGCCACGCTGGGATTTAAATCCATGAAGACGGCTTACGCCACCATCAAAGGTATTGAGGTGATGCGTGCACTACGCAAAGGCCAGG
CCTCAGCATTTTATTATGGTGATCCCCTGGGCGAAATGCGCCTGGTAAGCAGAGTTTTTGAAATGTAAGGCCTTTGAATAAGACAAAAGGCTGCCTCATC
GCTAACTTTGCAACAGTGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 705 bp | 234 aa | 64 | 768 | + | No |
Chemistry : DDE
ORF sequence :
MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGR
TVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIK
GIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
TVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIK
GIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
Blast result :
Comments
IS15 corresponds to the insertion of IS15-DI into IS15-DII. IS15DI and IS15DII differ by 4 bp (2 aa), IS15DI and IS26 by 3 bp, and IS15DII and IS26 by 1 bp. The major event mediated by IS15 and IS15D is the formation of cointegrates through replicative transposition. In Providencia stuartii BM2667, the amikacin-resistant Tn1528 consists of two direct copies of IS15D flanking the aph(3')-VIa gene, and is located on the pIP1848 conjugative plasmid. Tn1528 transposes via cointegrate formation, at a frequency of 4 x 10-5 in Escherichia coli (Lambert et al., 1994).
Found in Tn1525 and Tn1528.
Found in Tn1525 and Tn1528.
References