IS1536

  • Family IS607
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
Z97182 ND Mycobacterium tuberculosis
Mycobacterium tuberculosis complex
DNA section
IS Length : 1392 bp

Ends


IR Length :

IRL : GGGCTGCCAACTGTTGCGCGGTGAGTGGTCGGTAGTTGTCGGTGGCGTGC
IRR : GCCGTCTGGCACCAGGACCGGGCAGCATGCGCCGTCCGCGGTGGCGACCG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGGCTGCCAACTGTTGCGCGGTGAGTGGTCGGTAGTTGTCGGTGGCGTGCTGTAGGAACAGAGGTATGAATCTCGCGGCGTGGGCCGAGCGCAATGGCGT
CGCGCGGGTGACCGCGTATCGCTGGTTCCACGCTGGGCTCTTGCCGGTCCCGGCCCGGAAGGTTGGTCGACTCATTCTGGTCGACGAGCTGGCTAGCGAG
GCTGGCGCGCAGCCAAAGACTGCGGTGTACGCGCGGGTGTCGTCGGCTGATCAGAAGTCTGATTTGGATCGGCAGGTGGCGCGGGTGACTTCGTGGGCCA
CAGCCGAACAGATCCCGGTCGACAAGGTTGTCACCGAGGTCGGGTCGGTGCTCAACGGGCACCGACGTAAGTTCCCTGCGGTGCTGCGCGATCTGTCGGT
CACGCGGATTGTGGTTGAGCATCGGGATCGGTTCTGCCGGTTCGGTTCGGAGTATGTCCACGCTGCGCTGGCCGCTCAGGGTCGGGAGTTGGTCGTGGTG
GACTCGGCCGAGGTTGACGATGACCTGGTATGGGATATGACCGAGATTCTGACCTCGATGTGCGCAAGGTTGTATGGCAAACGTGCTGCTCAGAACCGGG
CCAAGCGGGCCGTCGCGGCTGCCGCTGTCGATGATCATGAGGCGGCCTGAGATGCCGCGTTTGGAGATCCCCAACGGCTGGTGTGTGCAAGCGTTCCGGT
TCACACTCGATCCGACCGCCGAGCAGGCACACGCGTTGGCGCGGCATTTCGGCGCCCGCCGCAAGGCCTACAACTGGACCGTCGCGCAGCTGAAAGCCGA
TATCCAAGCGTGGCGCGCGACCGGCGCCCAGACGGCGAAGCCGTCGCTTCGGGTACTGCGGAAACGCTGGAACACGGTGAAAGACGAGGTGTGTGTCAAC
GCCGAGACTGGCACCGTGTGGTGGCCGGAATGCTCGAAAGAGGCCTACGCCGACGGGATCGCGGGCGCGGTCGACGCGTACTGGAACTGGCAGCAGAGGC
GTGCTGGCAAGCGCGACGGCAAGAGAATGGGCTTCCCTCGATTCAAGAAGAAGGGCCGCGACGCCGATCGCGTGTCGTTCACCACGGGTGCGATGCGCGT
TGAGCCCGACCGTAGACACCTCACTTTGCCGGTGATCGGCTGCGTGCGTACGCATGAGAACACCCGCCGCATCGAGCGCCTCATCGCCAAAGACCGGGCG
CGGGTGCTGGCGATCACGGTGCGCCGCAACGGCACCCGGCTGGATGCGAGTGTGCGGGTACTGGTGCAGCGCCCCCAGCAACCCAACGTGGAACTGCCTG
AGTCGCGAATCGGTGTCGACGTGGGTGTTCGTCGTCTGGCCACGGTCGCCACCGCGGACGGCGCATGCTGCCCGGTCCTGGTGCCAGACGGC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
582 bp194 aa66647+No
ORF function : Transposase
Chemistry : Serine

ORF sequence :

MNLAAWAERNGVARVTAYRWFHAGLLPVPARKVGRLILVDELASEAGAQPKTAVYARVSSADQKSDLDRQVARVTSWATAEQIPVDKVVTEVGSVLNGHR
RKFPAVLRDLSVTRIVVEHRDRFCRFGSEYVHAALAAQGRELVVVDSAEVDDDLVWDMTEILTSMCARLYGKRAAQNRAKRAVAAAAVDDHEAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
744 bp247 aa6521395+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MPRLEIPNGWCVQAFRFTLDPTAEQAHALARHFGARRKAYNWTVAQLKADIQAWRATGAQTAKPSLRVLRKRWNTVKDEVCVNAETGTVWWPECSKEAYA
DGIAGAVDAYWNWQQRRAGKRDGKRMGFPRFKKKGRDADRVSFTTGAMRVEPDRRHLTLPVIGCVRTHENTRRIERLIAKDRARVLAITVRRNGTRLDAS
VRVLVQRPQQPNVELPESRIGVDVGVRRLATVATADGACCPVLVPDG

 

Blast result :
Comments
IS1536 appears to be truncated at the right-hand end as it looses homology with other members of the IS1535 group. M. tuberculosis H37Rv genome position: 701363-702754. The IS1535 group includes IS1535, IS1536, IS1537, IS1538, IS1539 and IS1602. The putative Tpase show between 40,6 and 86,1 % identity, they contained two overlapping ORFs where the stop codon from upstream ORF overlapped the start codon of the downstream ORF (TGATG). ORF1 shows similarity to certain resolvase. ORF2 shows weak similarity with IS1136 of Saccharopolyspora erythrae and IS891 of Anabaena, members of the IS605 family.
References
1] Cole, S.T. et al., Nature 393, 537-544
2] Gordon, S.V. et al, Microbiology 145, 881-892