ISfinder Mirror Site

IS1535

Family IS607
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
Z95210	ND	Mycobacterium tuberculosis	Mycobacterium tuberculosis complex

DNA section

IS Length : 2300 bp

Ends

IR Length : 15/16

IRL : TTGAGTGTGTTTTAGTGTGCGTTAGTGTGTTCTAATTGGCGGCGTGAATC
IRR : TTGAGTGTGTTTTAGTTGCACTCTCATGCGGCGTCCCCTTGCGGGGTTTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
			0

DNA sequence

TTGAGTGTGTTTTAGTGTGCGTTAGTGTGTTCTAATTGGCGGCGTGAATCTGGCGGATTGGGCGGAGTCGGTGGGGGTGAATCGACATACCGCTTATCGC
TGGTTTCGGGAGGGGACGTTGCCGGTGCCCGCGGAGCGGGTTGGCCGGTTGATCCTGGTCAAGACGGCCGCCTCGGCGTCGGCCGCAGCGGCGGGAGTGG
TGCTGTATGCGCGGGTGTCAAGCCATGATAGGCGTTCGGATCTGGATCGGCAGGTCGCGCGTCTAACCGCGTGGGCCACCGAGCGTGACTTGGGGGTGGG
GCAAGTGGTGTGCGAGGTCGGTTCCGGCCTGAACGGCAAGCGACCCAAGCTGCGGCGCATCTTGTCGGACCCCGATGCGAGAGTGATCGTTGTGGAGCAT
CGGGATCGGCTGGCGCGTTTCGGGGTGGAGCACCTCGAGGCGGCGCTGTCTGCTCAGGGCCGGCGGATTGTGGTCGCCGATCCTGGTGAGACGACCGATG
ATCTGGTGTGTGACATGATCGAGGTCTTGACCGGTATGTGCGCGCGGCTGTACGGGCGTCGCGGTGCGCGCAACCGGGCGATGCGTGCGGTCACGGAGGC
CAAGCGTGAGCCGGGGGCGGGGTGATGATCGTCAGGATGCGTAGCTGCGCTCAGGCCGCGAAGGTGGCCGAGGCCACCGGTGGTGTGCAGCTGGCGGGCA
AGCCGAAACCCGATGGGACACCGACGTTCTCCCGGTATGTGGAGATCGGCGTGGATTTTGAGGCGCACCGGCCGGTGGTGGAGTCGGTTTCGGTGCTGTT
CGAGCTTTATGACGGCGACGCCAACAGTTATGCCGCGACCGGGGGGCCGGGTGCCCAACTGCCGTCGGGCTGGATGGTCACGGCGGCGAAATTCGAGGTC
GAGTGGCCCGCCGACCCGCAGCGGGCGGGTTTGGTGCGTTCACATTTCGGCGCCCGCCGCAAAGCTTTCAACTGGGGCCTGGCCCAGGTGAAGGCCGACC
TCGACGCCAAAGCCGCTGATCCGGCACATGAGTCGGTGGACTGGGACTTGAAGTCGCTGCGATGGGCGTGGAACCGAGCCAAAGATGACGTGGCGCCGTG
GTGGGCCGAGAATTCCAAGGAGTGCTACTCGTCGGGGTTGGCCGATCTGGCCCAGGGCCTGGCTAATTGGAAAGCTGGCAAGAACGGGACCCGCAAAGGC
CGGCGGGTGGGCTTCCCGCGATTCAAATCCGGGCGGCGTGATCCTGGCAGGGTGCGGTTCACCACCGGCACCATGCGCATAGAGGATGACCGGCGCACGA
TCACGGTCCCGGTGATCGGGCCGCTGCGGGCCAAGGAGAACACCCGCCGGGTGCAACGCCACCTCGTGAGCGGGCGCGCGCAGATCCTGAACATGACCTT
GTCGCAGCGGTGGGGCCGGTTATTCGTGGCGGTCTGCTACGCGCTGCGCACCCCGACCACCAGATCACCGCTCACCCAGCCGACTGTGCGCGCCGGAATG
GACCTGGGAGTCCGGACCCTGGCCACGGTCGCCACCCTCGACACCGCCACCGGCGAGCAGACCATCATCGAATACCCAAACCCGGCCCCGCTCAAGGCGA
CACTCGTCGCCCGTCGCAGGGCCGGCCGAGAACTTTCCCGCCGCATCCCCGGCTCCCATGGGCATCGGGCAGTGAAAGCCAAGCTGGCCCGCCTGGATCG
CCGGTGCGTGCACCTACGGCGGGAAGCAGCCCACCAGCTCACCACCGAGTTGGCGGGCACCTATGGCCAGGTCGTGATCGAAGACCTCGACGTGGCCGCG
ATGAAACGCAGCATGCGCCGGCGGGCGTTTCGCCGATCGGTCTCCGATGCCGCAATGGGTTTGGTCGCGCCGCAGCTGGCTTACAAAACGGCCAAGTGCA
GCGGCGTGCTGACGGTGGCGGACCGCTGGTTTGCCTCCAGCCAAATCCACCACGGCTGCACCAGCCCCGACGGCACACCGTGCCGGCTGCAAGGCAAGGG
CCGCATCGACAAACACCTGCTCTGCCCTGTAACGGGCGAGGTAGTCGACCGCGACAGAAACGCTGCTTTGAATCTCCGTGACTGGCCGGATAACGCCAGT
CGTGGTCCAGTCGGGACCACGGCCCCATCGGCACCCGGGCCAACCACCACGGTTGGTACAGGCCATGGCGCGGACACCGGATCATCCGGCGCCGGCGGAG
CATCCGTAAGACCCCGCCCACGCAGGGCCGGACGCGGCGAGGCCAAAACCCAAACCCCGCAAGGGGACGCCGCATGAGAGTGCAACTAAAACACACTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
582 bp	193 aa	43	624	+	No

ORF function : Transposase
Chemistry : Serine

ORF sequence :

MNLADWAESVGVNRHTAYRWFREGTLPVPAERVGRLILVKTAASASAAAAGVVLYARVSSHDRRSDLDRQVARLTAWATERDLGVGQVVCEVGSGLNGKR
PKLRRILSDPDARVIVVEHRDRLARFGVEHLEAALSAQGRRIVVADPGETTDDLVCDMIEVLTGMCARLYGRRGARNRAMRAVTEAKREPGAG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1653 bp	550 aa	625	2277	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MIVRMRSCAQAAKVAEATGGVQLAGKPKPDGTPTFSRYVEIGVDFEAHRPVVESVSVLFELYDGDANSYAATGGPGAQLPSGWMVTAAKFEVEWPADPQR
AGLVRSHFGARRKAFNWGLAQVKADLDAKAADPAHESVDWDLKSLRWAWNRAKDDVAPWWAENSKECYSSGLADLAQGLANWKAGKNGTRKGRRVGFPRF
KSGRRDPGRVRFTTGTMRIEDDRRTITVPVIGPLRAKENTRRVQRHLVSGRAQILNMTLSQRWGRLFVAVCYALRTPTTRSPLTQPTVRAGMDLGVRTLA
TVATLDTATGEQTIIEYPNPAPLKATLVARRRAGRELSRRIPGSHGHRAVKAKLARLDRRCVHLRREAAHQLTTELAGTYGQVVIEDLDVAAMKRSMRRR
AFRRSVSDAAMGLVAPQLAYKTAKCSGVLTVADRWFASSQIHHGCTSPDGTPCRLQGKGRIDKHLLCPVTGEVVDRDRNAALNLRDWPDNASRGPVGTTA
PSAPGPTTTVGTGHGADTGSSGAGGASVRPRPRRAGRGEAKTQTPQGDAA

Blast result :

Comments

Mycobacterium tuberculosis H37Rv genome sequence position 1027039-1029361. The IS1535 group includes IS1535, IS1536, IS1537, IS1538, IS1539 and IS1602. The putative Tpase show between 40,6 and 86,1 % identity, they contained two overlapping ORFs where the stop codon from upstream ORF overlapped the start codon of the downstream ORF (TGATG). ORF1 shows similarity to certain resolvase. ORF2 shows weak similarity with IS1136 of Saccharopolyspora erythrae and IS891 of Anabaena, members of the IS605 family.
The ends of IS1535 but not the other elements carried perfect internal seventeen bp IRs.

References

1] Cole, ST et al. (1998) Nature 393, 537-544.
2] Gordon, SV et al (1999) Microbiology 145, 881-892.