IS1533

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s) IS1533W
Accession numberTranspositionOriginHost
M82880 ND Leptospira borgpetersenii
Leptospira interrogans serovar pomona type kennewicki RZ11
Leptospira borgpetersenii serovar hardjo type hardjo-bovis RZ33
Leptospira interrogans serovar icterohaemorrhagiae Verdun
DNA section
IS Length : 1460 bp

Ends


IR Length : 13/15

IRL : TGGCGCCCCTTCTGGGCTTGAAAAACGTGGGTTAGATGCGAGCATTTACC
IRR : TGGCGCCCCATCCGGAGCAGTAAATCTTTTTTCGCATATTTTTTAAATCA

Insertion site


Left flankDirect repeatRight flankDR Length
TTAGACAAAATATCAGANNN0

DNA sequence

TGGCGCCCCTTCTGGGCTTGAAAAACGTGGGTTAGATGCGAGCATTTACCACAAAACAAAAAAACCAACAGAAGGACGCGAATGAAAAGAAAAGTATATG
TAGGAATGGATGTCCACAAAGAAACGATCCAAATCGCGTATTTAACGAGCAATTCAAAGGAAATATTGAAAGAACAACAGATAAAACACAATGAAGTTCA
AATCAAAAAGTTCATCAAAAAACTAAAATTAGAATGGAATGAAATACATTGTTGTTACGAAGCGGGAGTAACGGGTTATCCACTTTACAGATATCTAAAG
TCATTGGGAGTGAACTGTATCCTTCTAGCGCCTGGAAAGATTCCAAGACAAAATACCGATAAGATCAAAACGGATAAAAGAGACGCGATCAAATTAGCGA
GATTAATGCGAAGCTGGAGAATTGGAATCGATTCATGTTTCCCAGTTGAAGAGGACGAAGCAGTAAGAGATTATTTAAGATCCCGTGACAGTCTTCGTTT
GGATTTAGGAAGGAATCGTCAAAGGTTGATGAAATTCTTATTAAGAAAAGACATAAAGCTACTTCCCACAACGAAGTATTGGACGGTGAGTCATTACAAA
TGGTTGAATAATCTTCACTTTAACAACGAGATCCTTCAAGAGACGTTTAACGATTATTATAGTCGAGTGAGAGTTCAAGAAGAGAATTTAAAAGCGATGG
ATAAAAAGATACAAGAGATAGCGGAAAGTGAACCGTATCGAGAGAAAGTGGGAATCTTAAGATGCTTTCGAGGAGTGGACTATTTAACGGCAATGTTCTT
ACTTTGTGAGGTAAACGACTTCAAGCCGATTCAAAACGGCCGGTTCGTTCATGAGTTTTCTTGGACTTGTTCCTGGAGAATATTCCAGCGGTTCCAAAAG
AAAACAAACAGGGATTACGTAAAACTGGAAGTTCCGAGACTTAGAAGGATTTTGACGGAAGCGGCTTGGCAACATCGCTTCCCTGGAACAGGAAGTAAAA
TCATAACCGCACGTAGATCGGGACAACCTGCATTAGTCGTTGCTTTAGCGGAAAAAGCTTCTCTCAGGTTACACAAGAAATTTCGCAATCTACAGCCTAA
GAGGAAAAAACTCCTCAAGTGATGATAACGGCAGTTTCAAGAGAGTTATCCGGATTTCTTTGGGCGGCAATGAATCTTGTAGCCTAAAGTTAGAGTAATG
TTCATCCAATAAATTGAATTCGTTAGAGAAGATGTACGATAAAGGAGGAATACTTGTTGGCGAGAGTGTTGAAGCAAATTTGAATTTCAAATTTGACCTT
CGTTTTTAGACTAAGAACAGCTCCCGATGTATAGATTATCCTTCCAACCCGCGAATATCAGTCTGATCAATCGTCGCGAATGCTTTTCTCTAACGAGTTA
AATTATTGGGTGATTTAAAAAATATGCGAAAAAAGATTTACTGCTCCGGATGGGGCGCCA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1041 bp346 aa821122+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MKRKVYVGMDVHKETIQIAYLTSNSKEILKEQQIKHNEVQIKKFIKKLKLEWNEIHCCYEAGVTGYPLYRYLKSLGVNCILLAPGKIPRQNTDKIKTDKR
DAIKLARLMRSWRIGIDSCFPVEEDEAVRDYLRSRDSLRLDLGRNRQRLMKFLLRKDIKLLPTTKYWTVSHYKWLNNLHFNNEILQETFNDYYSRVRVQE
ENLKAMDKKIQEIAESEPYREKVGILRCFRGVDYLTAMFLLCEVNDFKPIQNGRFVHEFSWTCSWRIFQRFQKKTNRDYVKLEVPRLRRILTEAAWQHRF
PGTGSKIITARRSGQPALVVALAEKASLRLHKKFRNLQPKRKKLLK

 

Blast result :
Comments
IS1533-related sequences are widely distributed among pathogenic leptospires. There are at least 40 copies of IS1533 in Leptospira borgpetersenii serovar hardjo. The length of IS1533 has been reevaluated; all coordinates have been changed accordingly. However, its limits remain uncertain. IS1533 is used as a specific probe for the detection of Leptospira borgpetersenii spirochetes in the urine of infected cattle. The ORF reported here resulted from a fusion of three ORFs (92-829, 831-911 and 911-1132), as suggested by their similarities to the other IS110 family transposases.
References
1] Zuerner, R.L., and Bolin, C.A. (1988) J. Clin. Microbiol. 26, 2495-2500.
2] Zuerner, R.L., Herrmann, J.L., and Saint Girons, I. (1993) J. Bacteriol. 175, 5445- 5451.
3] Zuerner, R.L. (1994) Plasmid 31, 1-11.
4] Woodward, M.J., and Sullivan, G.J. (1991) J. Gen. Microbiol. 137, 1101-1109.