IS1470
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X71844 | ND | Clostridium perfringens | Clostridium perfringens NCTC8239 |
DNA section
IS Length : 1212 bp
Ends
IR Length : 22/27
IRL : GCTTAATTGTAAAATGAAATTGAACTAAATAAAAAAATCCATGGTGGATT
IRR : TCTAAATTGCAATATCAAATTGAACTAGTCCTTAATTATGATTTAAGAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GCTTAATTGTAAAATGAAATTGAACTAAATAAAAAAATCCATGGTGGATTAACCTGTGTTATGATTTAATCGCGTAAAAAAATAAAACACACGGAGGATA
ATCACCATGGACTATCAAAATCATAACACAGAGTCAAGAAAAAATAAACACTTAAATATGAAAGAGCGTATGATAGTTGAAATACGTCTTAAAGATGGAT
TTTCAGCTTATAAAAATACAAAAGAACTAAATAGGCCTATAAATACCGTTTTAAACGAAATACGCCGTGGTACTACTAAGCAAATTAAGCAAGGTAAGGA
ATTTCATGTGTATTTTGCTGATACTGGTGAAGCTGTTTATAAGAAGAATCGCTTAAAATCTAATCGTAAATACAAACTATTAGAATGTAGTGATTTTATA
AAATATGTAGTTGATAAAGTTAAGAATGATCACTGGTCTTTAGATGCATGTGTTGGTGAAGCTCTGCATTCATCTAGATTTAGTCCTTCTCAAATTATTT
CAACAAAAACGCTTTATAACTATGTAGACCTTGGCTTATTACCTATAAAAAATATAGATTTACCAGCTAAACTTCATAGAAATAAAAAGTCTACTAGAGT
AAGAAATAATAAGAAAAAGCTTGGCACTAGCATTTCAGATCGTCCTAATTCTATTGAAAACCGTGAAGAATTTGGACATTGGGAAATAGACTGTGTATTA
GGTGAAAAATCTAATAAAGATAAGGTTTTGTTAACACTTGTCGAGCGTAAAACTAGGTATGCTATAATATCTGAAATGTCTTCCCATTCAACAATATCTG
TTACAAAGGCTCTTGATAAGATTAAAGAATTTTTGGGCAGTAAATTTAGTGAAGTATTTAAGAGTATAACTGCTGATAATGGCTCTGAATTTGCTGATTT
ATCAGAATTTGAACTAAAAACAAAAACTAAAGTGTATTTCACTCACCCATATTCTTCATTTGAAAAAGGTACAAATGAGCGTCATAATGGACTGATTAGG
CGTTTTATCCCGAAAGGAAAACGAATTTCAGATTACTCACTTGAAACTATATCTTTCATAGAAAATTGGATGAATACTCTTCCTAGAAAGCTTCTTGATT
ACAAGACTCCAGAAGAGTTATTTGAAATACACTTAGATAAAATATATTCATTATATTAATTAGTTCTTAAATCATAATTAAGGACTAGTTCAATTTGATA
TTGCAATTTAGA
ATCACCATGGACTATCAAAATCATAACACAGAGTCAAGAAAAAATAAACACTTAAATATGAAAGAGCGTATGATAGTTGAAATACGTCTTAAAGATGGAT
TTTCAGCTTATAAAAATACAAAAGAACTAAATAGGCCTATAAATACCGTTTTAAACGAAATACGCCGTGGTACTACTAAGCAAATTAAGCAAGGTAAGGA
ATTTCATGTGTATTTTGCTGATACTGGTGAAGCTGTTTATAAGAAGAATCGCTTAAAATCTAATCGTAAATACAAACTATTAGAATGTAGTGATTTTATA
AAATATGTAGTTGATAAAGTTAAGAATGATCACTGGTCTTTAGATGCATGTGTTGGTGAAGCTCTGCATTCATCTAGATTTAGTCCTTCTCAAATTATTT
CAACAAAAACGCTTTATAACTATGTAGACCTTGGCTTATTACCTATAAAAAATATAGATTTACCAGCTAAACTTCATAGAAATAAAAAGTCTACTAGAGT
AAGAAATAATAAGAAAAAGCTTGGCACTAGCATTTCAGATCGTCCTAATTCTATTGAAAACCGTGAAGAATTTGGACATTGGGAAATAGACTGTGTATTA
GGTGAAAAATCTAATAAAGATAAGGTTTTGTTAACACTTGTCGAGCGTAAAACTAGGTATGCTATAATATCTGAAATGTCTTCCCATTCAACAATATCTG
TTACAAAGGCTCTTGATAAGATTAAAGAATTTTTGGGCAGTAAATTTAGTGAAGTATTTAAGAGTATAACTGCTGATAATGGCTCTGAATTTGCTGATTT
ATCAGAATTTGAACTAAAAACAAAAACTAAAGTGTATTTCACTCACCCATATTCTTCATTTGAAAAAGGTACAAATGAGCGTCATAATGGACTGATTAGG
CGTTTTATCCCGAAAGGAAAACGAATTTCAGATTACTCACTTGAAACTATATCTTTCATAGAAAATTGGATGAATACTCTTCCTAGAAAGCTTCTTGATT
ACAAGACTCCAGAAGAGTTATTTGAAATACACTTAGATAAAATATATTCATTATATTAATTAGTTCTTAAATCATAATTAAGGACTAGTTCAATTTGATA
TTGCAATTTAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1053 bp | 350 aa | 107 | 1159 | + | No |
Chemistry : DDE
ORF sequence :
MDYQNHNTESRKNKHLNMKERMIVEIRLKDGFSAYKNTKELNRPINTVLNEIRRGTTKQIKQGKEFHVYFADTGEAVYKKNRLKSNRKYKLLECSDFIKY
VVDKVKNDHWSLDACVGEALHSSRFSPSQIISTKTLYNYVDLGLLPIKNIDLPAKLHRNKKSTRVRNNKKKLGTSISDRPNSIENREEFGHWEIDCVLGE
KSNKDKVLLTLVERKTRYAIISEMSSHSTISVTKALDKIKEFLGSKFSEVFKSITADNGSEFADLSEFELKTKTKVYFTHPYSSFEKGTNERHNGLIRRF
IPKGKRISDYSLETISFIENWMNTLPRKLLDYKTPEELFEIHLDKIYSLY
VVDKVKNDHWSLDACVGEALHSSRFSPSQIISTKTLYNYVDLGLLPIKNIDLPAKLHRNKKSTRVRNNKKKLGTSISDRPNSIENREEFGHWEIDCVLGE
KSNKDKVLLTLVERKTRYAIISEMSSHSTISVTKALDKIKEFLGSKFSEVFKSITADNGSEFADLSEFELKTKTKVYFTHPYSSFEKGTNERHNGLIRRF
IPKGKRISDYSLETISFIENWMNTLPRKLLDYKTPEELFEIHLDKIYSLY
Blast result :
Comments
The published sequence has recently been corrected (Brynestad et al., 1997). IS1470 is located about 73 bp upstream of IS1469, and is flanking the cpe (enterotoxin) gene in direct orientation. IS1470 transposase is 36 % identical to that of IS1161 from Streptococcus salivarius. There are at least 8 IS1470 copies in Clostridium perfringens NCTC8239, but the copy number varies from zero to more than 10 in different Clostridium perfringens strains.
References
1] Brynestad, S., Iwanejko, L., Stewart, GS., and Granum, P.E. (1994) Microbiol. 140, 97-104.
2] Brynestad, S., Synstad, B., and Granum, P.E. (1997) Microbiology 143, 2109-2115.
2] Brynestad, S., Synstad, B., and Granum, P.E. (1997) Microbiology 143, 2109-2115.