IS1383

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF052750 ND Pseudomonas putida
Pseudomonas putida H plasmid pPGH2
Pseudomonas putida H plasmid pPGH1
DNA section
IS Length : 1419 bp

Ends


IR Length : 13

IRL : agatggTATGGACGCCTCCCCCCGGCAGCGGCCGACCGCAGCACCTCTAC
IRR : ----taTATGGACGCCTCCCGGAGCTCAATAGGTAGCCATGCTGGCGCGT

Insertion site


Left flankDirect repeatRight flankDR Length
TTCAGATGGTATAAG0

DNA sequence

AGATGGTATGGACGCCTCCCCCCGGCAGCGGCCGACCGCAGCACCTCTACATCAATAGAGGATCCTCGCTGCCCCGGCATCTAGGTGCCAAAGTGGAGAG
TGGATAACAAACACCACTTGAGCAGGAGGATCCACCATGAAACATAGCGTAATTGGCATGGACATCGCCAAGAAGGTTTTCCAGCTGCACACCGTTGATC
AGTGCACTGGGAGGATCGAGCGTATCAAGCTGCGCCGTGATGAAGTGCTGGCGTTCTTTGCAAATTATCCGACCAGCCTGGTGGCAATCGAGGCCTGTGG
CAGTGCCCACTGGTGGGCACGTCAGCTTCAACAGCAGGGGCATGAGGTACGACTACTCGCCCCTCGTTCAGTACGCCCCTTTGTGCTGCGAAACAAAACC
GATGCGGCTGACGCTCAAGCCATCTGGACAGCGGTGCAGCAGCCCGGCGCTTGCCTGGTGGCAATCAAGCAGGCGGATCAGCAGGCGATCCTCTCGCTAC
ACCGTATCCGCTCCCAGTTGCTGAAATTTCGCATCATGCAGAGCAATGGCTTACGTGGGCTGCTTTACGAATTCGGAATCGTGCTGCCAGAAGGTTATAC
CCAGCTGAGCAAGGCCGTTCCGGAGGCCTTCGCCGATGCTGAGCATCGAGTGCCTTCCATGTTGTTGGACAGCCTACGCGATCAGTGGACTCGGATTATC
CAGTTGGATGAGGAAATTCGAAAAATCGAACTGCGTTTGAAACAGTGCCTGCGTGAGAGCTCCGACTGCCAGAAAATTGCAGAGATTCCCGGAATTGGTT
TGTTGACCGCTACAGCCGCTGTTGCCTCACTGGGAGATGCGACAACCTTCCATTCTGGTCGCCAGTTCGCGGCTTGGCTGGGACTGGTTCCTCGACAAAC
GGGCACCGGTGGCAGAGTTCGGCAACTGGGGCTGAGCAAGCGCGGAGACACCTATCTGCGAAGTCTGTTGATGCATGGTGCTCGCTCGATCCTCAATAGA
GGACACCGGTCAGAGTGGGTTGAACGCTTGTTGACCAGGCGGCCTTATAACGTGGCGGTCGCCGCAGTGGCTAACAAGCTCGCCAGAACCCTTTGGGCCG
TGTTGGCCAAGGGCTCTACGTATCGGGCCGAACTCTTTACGGCATGCCCTACGGGGCATTGAAAACAGTAACCAACAGACTTCACAAGGAGCACAGGCGG
TAAGCACGTGATGGCGAAATAGGTCAGACCGTGACGGGGTAAACCTGGTAGGGATATGAGCCACGGGCACAGCCTTCAGCTCGATTTTCAGATGAGGGCC
TCGTCAGCGGATTCCATCAGGGCCAGCAGGTTTTTGCCTGCACAACAGGCCGGATATAAGACTGCTCCTACCTACGCGCCAGCATGGCTACCTATTGAGC
TCCGGGAGGCGTCCATATA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1026 bp341 aa1371162+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MKHSVIGMDIAKKVFQLHTVDQCTGRIERIKLRRDEVLAFFANYPTSLVAIEACGSAHWWARQLQQQGHEVRLLAPRSVRPFVLRNKTDAADAQAIWTAV
QQPGACLVAIKQADQQAILSLHRIRSQLLKFRIMQSNGLRGLLYEFGIVLPEGYTQLSKAVPEAFADAEHRVPSMLLDSLRDQWTRIIQLDEEIRKIELR
LKQCLRESSDCQKIAEIPGIGLLTATAAVASLGDATTFHSGRQFAAWLGLVPRQTGTGGRVRQLGLSKRGDTYLRSLLMHGARSILNRGHRSEWVERLLT
RRPYNVAVAAVANKLARTLWAVLAKGSTYRAELFTACPTGH

 

Blast result :
Comments
Six copies identified in pPH1 and pPGH2 have been sequenced.
Although a significant sequence identity has been observed at each site, no unoccupied sites have been compared.
All six gave the following sequence : TTCAGATGGT(IS)ATAAG. This either demonstrates target specificity or perhaps that this sequence is part of the IS.
The IR of this IS are not at its termini. In the IS sequence as given7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.

Six copies of IS1383 identified in pPGH1 and pPGH2 have been sequenced.

The transposase protein is 87% identical to that of ISAzvi3 and 38% identical to that of IS1111.

IS1383 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Lauf et al. (1999) FEMS Microbiol. Lett. 170, 407-412
2] Muller et al. (2001) Mol. Genet. Genomics 265, 1004-1010
3] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384