IS1373
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U05249 | Y | Streptomyces lividans | Streptomyces lividans 66 1326.32 |
DNA section
IS Length : 817 bp
Ends
IR Length : 9/15
IRL : GAGGTTGTCCCGTAACCAATGGTGCGGCTGGTGCGTTGGTCGGGCATGGG
IRR : CGAGTTATCCAGTGAGGGCCAGGTTGTGCAGTCGGGCGATGCCGAGCATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATAACAGACC | TA | GGAAAAGCAT | 2 |
| CTTCGTGCCC | TA | GGAAAAGCAT | 2 |
| CTTCGTGCCC | TA | GGGCGGATGG | 2 |
DNA sequence
GAGGTTGTCCCGTAACCAATGGTGCGGCTGGTGCGTTGGTCGGGCATGGGTGGGGTGATCTCAGCAGATGATCCGAAGTGGATCGAGCCGTTTGCGGGTC
TGACCGAGGTGCAGTTCGGGAGGCTGGTGGCACTGGTGCGGCGCCGAGGTGGCGACGTTCAGCGTGGCCGGCCGTGGCGGCTGTCGCTCGAGGACCGGGT
GTTGCTGGTGGCGACGTACTGGCGCACGAACCTCACATTGCGGCAGGTGGCACCGTTGTTCGGAGTCTCGAAGTCCGCGGCCGACCGCATCTTGGACCAT
CTCGCACCGCTGCTGGCCATCTCGCCCGCACGCCGGCCGCGCAAGGACACCGTCTACATTGTCGACGGCACCCTGGTGCCCACGCGCGACCGCAATGTCG
CCGCGTCCAGCAAGAACTACCGGTACTCGACCAACCTGCAGGTCGTCATCGACGCCAACAGCCGCTTGGTCGTCGCCATCGGTCTCCCGTTGCCCGGCAG
CCGCAACGACTGCCGGGCGTTCACGGAGTCCGGCATCGATCGGGCCTGCCGCGGCGCCCCAACCATCGCCGACGGCGGCTACCAGGGCACTGGTCTCCTC
ATTCCGCACCGAAGACGACGAGGGCAGAGCCACCTCAGCCCATCGCAGGAGGCAGAGAACGCTGTCCATCGCCGGGCACGAGCGCGGGTGGAACATGCCC
TTTCGCGGTTGAAGAACTGGAAGATCCTGCGGGACTGCCGGCTCAAGGGCGACGGAGTTCACCAGGCCATGCTCGGCATCGCCCGACTGCACAACCTGGC
CCTCACTGGATAACTCG
TGACCGAGGTGCAGTTCGGGAGGCTGGTGGCACTGGTGCGGCGCCGAGGTGGCGACGTTCAGCGTGGCCGGCCGTGGCGGCTGTCGCTCGAGGACCGGGT
GTTGCTGGTGGCGACGTACTGGCGCACGAACCTCACATTGCGGCAGGTGGCACCGTTGTTCGGAGTCTCGAAGTCCGCGGCCGACCGCATCTTGGACCAT
CTCGCACCGCTGCTGGCCATCTCGCCCGCACGCCGGCCGCGCAAGGACACCGTCTACATTGTCGACGGCACCCTGGTGCCCACGCGCGACCGCAATGTCG
CCGCGTCCAGCAAGAACTACCGGTACTCGACCAACCTGCAGGTCGTCATCGACGCCAACAGCCGCTTGGTCGTCGCCATCGGTCTCCCGTTGCCCGGCAG
CCGCAACGACTGCCGGGCGTTCACGGAGTCCGGCATCGATCGGGCCTGCCGCGGCGCCCCAACCATCGCCGACGGCGGCTACCAGGGCACTGGTCTCCTC
ATTCCGCACCGAAGACGACGAGGGCAGAGCCACCTCAGCCCATCGCAGGAGGCAGAGAACGCTGTCCATCGCCGGGCACGAGCGCGGGTGGAACATGCCC
TTTCGCGGTTGAAGAACTGGAAGATCCTGCGGGACTGCCGGCTCAAGGGCGACGGAGTTCACCAGGCCATGCTCGGCATCGCCCGACTGCACAACCTGGC
CCTCACTGGATAACTCG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 768 bp | 255 aa | 46 | 813 | + | No |
Chemistry : DDE
ORF sequence :
MGGVISADDPKWIEPFAGLTEVQFGRLVALVRRRGGDVQRGRPWRLSLEDRVLLVATYWRTNLTLRQVAPLFGVSKSAADRILDHLAPLLAISPARRPRK
DTVYIVDGTLVPTRDRNVAASSKNYRYSTNLQVVIDANSRLVVAIGLPLPGSRNDCRAFTESGIDRACRGAPTIADGGYQGTGLLIPHRRRRGQSHLSPS
QEAENAVHRRARARVEHALSRLKNWKILRDCRLKGDGVHQAMLGIARLHNLALTG
DTVYIVDGTLVPTRDRNVAASSKNYRYSTNLQVVIDANSRLVVAIGLPLPGSRNDCRAFTESGIDRACRGAPTIADGGYQGTGLLIPHRRRRGQSHLSPS
QEAENAVHRRARARVEHALSRLKNWKILRDCRLKGDGVHQAMLGIARLHNLALTG
Blast result :
Comments
IS1373 is part of an amplified DNA sequence called ADS2 (Amplified DNA Sequences); in fact, it is flanking an Amplifiable Units of DNA, the AUD2 sequence. By analogy to other IS5 members, the ends of IS1373 have been set to start with GAGG, compatible with DR of 2 bp. This assumption remains to be confirmed. IS1373 putative transposase is 68 % identical to that of IS112.
References
1] Eichenseer, C., and Altenbuchner, J. (1994) J. Bacteriol. 176, 7107-7112.
2] Volff, J.-N., and Altenbuchner, J. (1997) Submitted.
2] Volff, J.-N., and Altenbuchner, J. (1997) Submitted.