IS1249
- Family IS256
- Group IS1249
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U21300 | Y | Corynebacterium xerosis | Corynebacterium urealyticum DSM 7109 Corynebacterium xerosis M82B plasmid pTP10 Corynebacterium jeikeium CJ21 Propionibacterium acnes Corynebacterium striatum Bifidobacterium thermophilum |
DNA section
IS Length : 1385 bp
Ends
IR Length : 27/31
IRL : GGGTTAAGGGTCAAAAAGTGTGTCCGGAATCGCGGATTTTCACGGATTGA
IRR : GGGTTAAGGGTCAAAATGTGTGTCTGGCTCGGCGTGTCGCGGGGGTTAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTTGAGCTG | TGTAAACC | CAAGTCCCTA | 8 |
| TGTTGAAGAA | GGTTTTGG | CGTTCTGGGG | 8 |
| GGGGTACCCA | GAAAGATC | CTCAAAAAGT | 8 |
| NNNNNNNNNN | GAAAGCCC | NNNNNNNNNN | 8 |
DNA sequence
GGGTTAAGGGTCAAAAAGTGTGTCCGGAATCGCGGATTTTCACGGATTGACCTGTAGGTTTCCGGAAAGTATATGCTCCGGAAGCATATATCTAGCCCCC
GACGGGAAATCCTCGGTGTGGCAGCCGTGATGTGCTATGCGCGGCGTGGTGTGGTGTCGCAATGTCGAAGAACCAACCACGCTGCCACTGCGGCGGTGAA
ATGAAACGCAACGGCACCACCAGCAAAGGCACCACCAGATGGCGCTGCAAACAATGCGGCGCCTCCAGCGTCAAACGTCGAAACGACATCACCAACGCGG
CAGTGTTCACCCAGTTCATCGAGCATTGCACCACCGCAATATCACTCGACGACCTAGCCAAACGAAACGGTGTTAGCCGCGCCACCATGAAGCGGCGCTT
CAAGTGGTGCTGGCTCGTTGATGTGCCTGACCCCACCGCCGGCCACCACAAGCGGATCTACGACCAGGTATTTCTCGATGGCACCTACACCGCCGGTGGC
TGCCTGATCGTCGCGGCGACCATCGACCACGTGATCGCCTGGCACTGGTGCAAACACGAAACCACCCGCGACTACCAACTGCTGCTTGAACGCATCGAAG
CCCCACTCATCGCCGTCATCGACGGCGGCCAAGGCGCATACAGCGCAATCAAAAAGTGCTGGCCGACTACGAAAATTCAACGCTGCCTCGTCCACGCCCA
ACGCGTGGTCCGCCGCTACACCACCACCAACCCACGCACCGATGCCGGGCGCACCATCTACCGACTTGCGCTGAAACTGACCCGGATCACCACACTGGAT
GAAGCCGCCGCGTGGGGTGTGCAACTGCACGAGTTTTCAACGATCTACCGGGAATGGATGAACGAGAAAACCATGATCAAAGACCCCAAAACAGGTGCAT
GGACCCGCGTGTGGACCCACCACAACGTGCGCAAGGCCTACAACAGCCTCAACCATCTTTGGCGGTCCGAGATGCTGTTTGTCTACCTCAACCCGCCAGC
AGGAGTCCTTGCGCCCGAGCGGATCAAATCCACCACCAACAGCTTAGAAGGCGGCATCAACGCCCAGCTCAAACTGCTCGCCAGAACCCACCGCGGCAGA
TCAGGCGAACGACAACGCCGCATGCTGGATTGGTGGCTCTACTTAAAAACGGAACTGCCTGACGATCCAGTACGAATCGCCAGGCAGTCCGACTGGGGCC
AGGGCCAACTCGCCAAAGTATCCACCCTGACCCAAACCGAGAACCAAGCCGACCACGAAACAGGACGCCCAGCCCTCTACGACAACGCTATCGACACCGA
CTACACCCACTCAATCGGCATTCAAAAAGGCCAAATCTAACCCCCGCGACACGCCGAGCCAGACACACATTTTGACCCTTAACCC
GACGGGAAATCCTCGGTGTGGCAGCCGTGATGTGCTATGCGCGGCGTGGTGTGGTGTCGCAATGTCGAAGAACCAACCACGCTGCCACTGCGGCGGTGAA
ATGAAACGCAACGGCACCACCAGCAAAGGCACCACCAGATGGCGCTGCAAACAATGCGGCGCCTCCAGCGTCAAACGTCGAAACGACATCACCAACGCGG
CAGTGTTCACCCAGTTCATCGAGCATTGCACCACCGCAATATCACTCGACGACCTAGCCAAACGAAACGGTGTTAGCCGCGCCACCATGAAGCGGCGCTT
CAAGTGGTGCTGGCTCGTTGATGTGCCTGACCCCACCGCCGGCCACCACAAGCGGATCTACGACCAGGTATTTCTCGATGGCACCTACACCGCCGGTGGC
TGCCTGATCGTCGCGGCGACCATCGACCACGTGATCGCCTGGCACTGGTGCAAACACGAAACCACCCGCGACTACCAACTGCTGCTTGAACGCATCGAAG
CCCCACTCATCGCCGTCATCGACGGCGGCCAAGGCGCATACAGCGCAATCAAAAAGTGCTGGCCGACTACGAAAATTCAACGCTGCCTCGTCCACGCCCA
ACGCGTGGTCCGCCGCTACACCACCACCAACCCACGCACCGATGCCGGGCGCACCATCTACCGACTTGCGCTGAAACTGACCCGGATCACCACACTGGAT
GAAGCCGCCGCGTGGGGTGTGCAACTGCACGAGTTTTCAACGATCTACCGGGAATGGATGAACGAGAAAACCATGATCAAAGACCCCAAAACAGGTGCAT
GGACCCGCGTGTGGACCCACCACAACGTGCGCAAGGCCTACAACAGCCTCAACCATCTTTGGCGGTCCGAGATGCTGTTTGTCTACCTCAACCCGCCAGC
AGGAGTCCTTGCGCCCGAGCGGATCAAATCCACCACCAACAGCTTAGAAGGCGGCATCAACGCCCAGCTCAAACTGCTCGCCAGAACCCACCGCGGCAGA
TCAGGCGAACGACAACGCCGCATGCTGGATTGGTGGCTCTACTTAAAAACGGAACTGCCTGACGATCCAGTACGAATCGCCAGGCAGTCCGACTGGGGCC
AGGGCCAACTCGCCAAAGTATCCACCCTGACCCAAACCGAGAACCAAGCCGACCACGAAACAGGACGCCCAGCCCTCTACGACAACGCTATCGACACCGA
CTACACCCACTCAATCGGCATTCAAAAAGGCCAAATCTAACCCCCGCGACACGCCGAGCCAGACACACATTTTGACCCTTAACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1209 bp | 402 aa | 132 | 1340 | + | No |
Chemistry : DDE
ORF sequence :
MLCAAWCGVAMSKNQPRCHCGGEMKRNGTTSKGTTRWRCKQCGASSVKRRNDITNAAVFTQFIEHCTTAISLDDLAKRNGVSRATMKRRFKWCWLVDVPD
PTAGHHKRIYDQVFLDGTYTAGGCLIVAATIDHVIAWHWCKHETTRDYQLLLERIEAPLIAVIDGGQGAYSAIKKCWPTTKIQRCLVHAQRVVRRYTTTN
PRTDAGRTIYRLALKLTRITTLDEAAAWGVQLHEFSTIYREWMNEKTMIKDPKTGAWTRVWTHHNVRKAYNSLNHLWRSEMLFVYLNPPAGVLAPERIKS
TTNSLEGGINAQLKLLARTHRGRSGERQRRMLDWWLYLKTELPDDPVRIARQSDWGQGQLAKVSTLTQTENQADHETGRPALYDNAIDTDYTHSIGIQKG
QI
PTAGHHKRIYDQVFLDGTYTAGGCLIVAATIDHVIAWHWCKHETTRDYQLLLERIEAPLIAVIDGGQGAYSAIKKCWPTTKIQRCLVHAQRVVRRYTTTN
PRTDAGRTIYRLALKLTRITTLDEAAAWGVQLHEFSTIYREWMNEKTMIKDPKTGAWTRVWTHHNVRKAYNSLNHLWRSEMLFVYLNPPAGVLAPERIKS
TTNSLEGGINAQLKLLARTHRGRSGERQRRMLDWWLYLKTELPDDPVRIARQSDWGQGQLAKVSTLTQTENQADHETGRPALYDNAIDTDYTHSIGIQKG
QI
Blast result :
Comments
Tn5432 (4,524 bp) consists of two identical copies of IS1249 flanking the erythromycin resistance gene ermCX (Tauch et al., 1995). IS1249 is the more distant member of the IS256 family. Although its IR fit well with those of the other members, its putative transposase (402 aa) displays similarities with that of the other family members in its central region only (residues 113-315). Moreover, this sub-domain is about 30-aa longer for IS1249, due to the presence of an "inserted" segment at around position 225.
99% aa similar to IS1249 of Corynebacterium jeikeium CJ21 (Accession number AF338705 ), position 338-339 there are GF instead of RI.
Additionnal accession number : AF411029 , Propionibacterium acnes , 99% aa similar to IS1249.
99% aa similar to IS1249 of Corynebacterium jeikeium CJ21 (Accession number AF338705 ), position 338-339 there are GF instead of RI.
Additionnal accession number : AF411029 , Propionibacterium acnes , 99% aa similar to IS1249.
References