IS1216E
- Family IS6
- Group
Isoform IS1216Synonym(s) ISS1Y, ISEfa14
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U49512 | ND | Enterococcus faecium | Enterococcus faecalis CH19 Enterococcus faecium HM1073 |
DNA section
IS Length : 808 bp
Ends
IR Length : 24
IRL : GGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAAGGTAGAATAATAG
IRR : GGTTCTGTTGCAAAGTTTTAAATAAAGAATAAAATCCTTACGGTATCTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAAGGTAGAATAATAGAAAAAGATAGCAGGAGGAATGACGATGAATCATTTTAAAGGAAAGCAATT
TCAGCAGGATGTGATTATTGTAGCCGTGGGCTACTATCTTCGTTATAACCTTAGCTATCGTGAAGTTCAAGAAATCTTATATGATCGTGGCATTAACGTT
TCTCATACGACGATTTATCGTTGGGTGCAAGAATATGGCAAACTACTCTATCAAATTTGGAAAAAGAAAAATAAAAAATCCTTTTATTCATGGAAAATGG
ATGAAACGTACATCAAAATTAAAGGAAAATGGCATTATTTGTATCGAGCCATCGATGCAGATGGTTTAACCTTGGATATTTGGTTACGTAAAAAACGGGA
CACACAAGCAGCCTATGCTTTTCTTAAGCGGTTAGTGAAGCAGTTTGATGAACCGAAGGTTGTAGTCACAGATAAAGCCCCCTCTATTACAAGTGCCTTT
AAGAAACTAAAAGAATACGGCTTTTATCAAGGGACAGAACATCGTACCATTAAATACCTGAATAATTTGATTGAACAAGACCATCGTCCAGTAAAGAGAC
GCAATAAATTCTATCGAAGTTTACGCACTGCCTCTACCACGATTAAAGGCATGGAAGCCATTCGAGGATTATATAAGAAAACCCGAAAAGAAGGCACTCT
CTTCGGGTTTTCGGTCTGTACTGAAATCAAGGTATTATTGGGAATCCCAGCTTAAATCATAGATACCGTAAGGATTTTATTCTTTATTTAAAACTTTGCA
ACAGAACC
TCAGCAGGATGTGATTATTGTAGCCGTGGGCTACTATCTTCGTTATAACCTTAGCTATCGTGAAGTTCAAGAAATCTTATATGATCGTGGCATTAACGTT
TCTCATACGACGATTTATCGTTGGGTGCAAGAATATGGCAAACTACTCTATCAAATTTGGAAAAAGAAAAATAAAAAATCCTTTTATTCATGGAAAATGG
ATGAAACGTACATCAAAATTAAAGGAAAATGGCATTATTTGTATCGAGCCATCGATGCAGATGGTTTAACCTTGGATATTTGGTTACGTAAAAAACGGGA
CACACAAGCAGCCTATGCTTTTCTTAAGCGGTTAGTGAAGCAGTTTGATGAACCGAAGGTTGTAGTCACAGATAAAGCCCCCTCTATTACAAGTGCCTTT
AAGAAACTAAAAGAATACGGCTTTTATCAAGGGACAGAACATCGTACCATTAAATACCTGAATAATTTGATTGAACAAGACCATCGTCCAGTAAAGAGAC
GCAATAAATTCTATCGAAGTTTACGCACTGCCTCTACCACGATTAAAGGCATGGAAGCCATTCGAGGATTATATAAGAAAACCCGAAAAGAAGGCACTCT
CTTCGGGTTTTCGGTCTGTACTGAAATCAAGGTATTATTGGGAATCCCAGCTTAAATCATAGATACCGTAAGGATTTTATTCTTTATTTAAAACTTTGCA
ACAGAACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 681 bp | 226 aa | 75 | 755 | + | No |
Chemistry : DDE
ORF sequence :
MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTL
DIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLY
KKTRKEGTLFGFSVCTEIKVLLGIPA
DIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLY
KKTRKEGTLFGFSVCTEIKVLLGIPA
Blast result :
Comments
IS1216 and IS1216E ORFs are 99 % identical.
July 18 2012 : the file of ISS1Y (partial sequence) was deleted and the information were added to IS1216E file :
In Enterococcus faecalis CH19, the chromosomal beta-lactamase gene is located ca. 600 bp downstream of an IS256 copy (IS256E), and upstream of an iso-ISS1W (95.5 % identity on 133 aa), named here ISS1Y, that has partially been sequenced (Rice and Marshall, 1994). ISS1Y transposase is 95.5 % identical to that of IS1216.
July 18 2012 : the file of ISS1Y (partial sequence) was deleted and the information were added to IS1216E file :
In Enterococcus faecalis CH19, the chromosomal beta-lactamase gene is located ca. 600 bp downstream of an IS256 copy (IS256E), and upstream of an iso-ISS1W (95.5 % identity on 133 aa), named here ISS1Y, that has partially been sequenced (Rice and Marshall, 1994). ISS1Y transposase is 95.5 % identical to that of IS1216.
References
1] Arthur, M. (1996) "The VanS sensor negatively controls VanR-mediated transcriptional activation of glycopeptide resistance genes of Tn1546 and related elements". EMBL Data Library, Rel. 48.
2] Rice, L.B., and Marshall, S.H. (1994) Antimicrob. Agents Chemother. 38, 693-701.
2] Rice, L.B., and Marshall, S.H. (1994) Antimicrob. Agents Chemother. 38, 693-701.