IS1186
- Family IS5
- Group IS5
Isoform IS1168Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X72301 | Y | Bacteroides fragilis | Bacteroides fragilis BFr81R |
DNA section
IS Length : 1320 bp
Ends
IR Length : 13/17
IRL : GGGGACTTCTATAAATTAAATCCCTGATTATAAGTTGCATAAGCGGATAA
IRR : GGTGGGTTCTATCAATTCCATTTTTTATTTCTTGCATAAGACAGCAGAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAAATAAAA | AGAA | TAAAATGAAA | 4 |
| TTAGTTTGAA | TACA | GCAAATAAAA | 4 |
DNA sequence
GGGGACTTCTATAAATTAAATCCCTGATTATAAGTTGCATAAGCGGATAAAATGCTTATATTTGCAGAAACAGACAAAATAATCATAGATCATGAACAAG
TTAAGCCGCTATCGCAAGTTACGCTATAAAAGTGTGTTTGAATCCGAGAATCAATCGTCTCGCCTTAACGTGATAGGCAACCCACTAGAGAGACTGTCGT
CATTGGTGGACTTTGAGATATTCCGTCCGACATTGGAAGCCTCTCTGTTCACAGGCGAGCGTAAGGGCAATGCCGGGCGTCCTCCCATAGATTGTGTGTT
GATGTTCAAGATACTGTTTCTCCAGCGTTACTATGGCCTGAGTGACCATCAGATAGAATATCAGATAGTGGACCGCACCAGTTTCCGCGCTTTTCTGGGG
ATAGAGAACGTGGATGATGTACCGGACGAGAAGACAATCTGGAAGTATCGCGAAGCCCTCACGTCCGTCGGTGCGTATGACAAGTTGTTTGAAGACTTCC
GTACGTTCATGGAGGCCAAGGGCCTTCAGTTCAACGAGGGGCGGATCATAGATGCCAGTTTTGTCGTTGCCCCACGCCAGCGCAACACTCGTGAGGAGAA
TGCGAAGATAAAGGAGGGCAAGGGCGATGAACTGTGGAATGACAATCCTCACAAGAAATTCCATAAGGATGTGGATGCCCGTTGGACGGAGAAGCGCGGA
GACACGTTTTACGGCTACAAGCAGCATGTCAAGGTAGACAAAGGCAACAAGGTAATCCTCTCGTATGCAACAACGCCTGCCAATGTGCATGATTCCAAAG
GTTTTGAGCAGCTGCTTGATGAGTCAGACAAGGATAAGGATTTGTATCTTGACGCAGGATATGCCGGGCAGGAGTCAACCGTGAAGGAGCATGGCATGAA
TCCGATAATCTGCGAGAAAGGCCGTCGGAATCATCCTCTGACAGAAAAACAGAAGGCTGAGAACAGGCGCAAGTCCAAGACCCGTTGCCTTGTCGAGCAC
GTATTCGGTTTTGAAGAGCAAAGTATGCACGGCCTTATTGTCAGGACAATAGGGATTGTACGGGCAAAGGCGAATGTGGCGATGACAAATTTGACCTACA
ACATCTTCCGCTACATCCAGATTGTGTGCAATAAACGTGAATTGGCGATTGCCCAATAAGAAGAGACGGGGTGTATCCTCAAAAATGCAAAAATCCCTCA
TTTTTTTTTGGGGGGGGAGATTAAATTGCTTAACTTTACGCAAGATTATCTTGCTCAAGACAGTTAAAGGGTTCTGCTGTCTTATGCAAGAAATAAAAAA
TGGAATTGATAGAACCCACC
TTAAGCCGCTATCGCAAGTTACGCTATAAAAGTGTGTTTGAATCCGAGAATCAATCGTCTCGCCTTAACGTGATAGGCAACCCACTAGAGAGACTGTCGT
CATTGGTGGACTTTGAGATATTCCGTCCGACATTGGAAGCCTCTCTGTTCACAGGCGAGCGTAAGGGCAATGCCGGGCGTCCTCCCATAGATTGTGTGTT
GATGTTCAAGATACTGTTTCTCCAGCGTTACTATGGCCTGAGTGACCATCAGATAGAATATCAGATAGTGGACCGCACCAGTTTCCGCGCTTTTCTGGGG
ATAGAGAACGTGGATGATGTACCGGACGAGAAGACAATCTGGAAGTATCGCGAAGCCCTCACGTCCGTCGGTGCGTATGACAAGTTGTTTGAAGACTTCC
GTACGTTCATGGAGGCCAAGGGCCTTCAGTTCAACGAGGGGCGGATCATAGATGCCAGTTTTGTCGTTGCCCCACGCCAGCGCAACACTCGTGAGGAGAA
TGCGAAGATAAAGGAGGGCAAGGGCGATGAACTGTGGAATGACAATCCTCACAAGAAATTCCATAAGGATGTGGATGCCCGTTGGACGGAGAAGCGCGGA
GACACGTTTTACGGCTACAAGCAGCATGTCAAGGTAGACAAAGGCAACAAGGTAATCCTCTCGTATGCAACAACGCCTGCCAATGTGCATGATTCCAAAG
GTTTTGAGCAGCTGCTTGATGAGTCAGACAAGGATAAGGATTTGTATCTTGACGCAGGATATGCCGGGCAGGAGTCAACCGTGAAGGAGCATGGCATGAA
TCCGATAATCTGCGAGAAAGGCCGTCGGAATCATCCTCTGACAGAAAAACAGAAGGCTGAGAACAGGCGCAAGTCCAAGACCCGTTGCCTTGTCGAGCAC
GTATTCGGTTTTGAAGAGCAAAGTATGCACGGCCTTATTGTCAGGACAATAGGGATTGTACGGGCAAAGGCGAATGTGGCGATGACAAATTTGACCTACA
ACATCTTCCGCTACATCCAGATTGTGTGCAATAAACGTGAATTGGCGATTGCCCAATAAGAAGAGACGGGGTGTATCCTCAAAAATGCAAAAATCCCTCA
TTTTTTTTTGGGGGGGGAGATTAAATTGCTTAACTTTACGCAAGATTATCTTGCTCAAGACAGTTAAAGGGTTCTGCTGTCTTATGCAAGAAATAAAAAA
TGGAATTGATAGAACCCACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1068 bp | 355 aa | 253 | 1320 | + | No |
Chemistry : DDE
ORF sequence :
MNKLSRYRKLRYKSVFESENQSSRLNVIGNPLERLSSLVDFEIFRPTLEASLFTGERKGNAGRPPIDCVLMFKILFLQRYYGLSDHQIEYQIVDRTSFRA
FLGIENVDDVPDEKTIWKYREALTSVGAYDKLFEDFRTFMEAKGLQFNEGRIIDASFVVAPRQRNTREENAKIKEGKGDELWNDNPHKKFHKDVDARWTE
KRGDTFYGYKQHVKVDKGNKVILSYATTPANVHDSKGFEQLLDESDKDKDLYLDAGYAGQESTVKEHGMNPIICEKGRRNHPLTEKQKAENRRKSKTRCL
VEHVFGFEEQSMHGLIVRTIGIVRAKANVAMTNLTYNIFRYIQIVCNKRELAIAQ
FLGIENVDDVPDEKTIWKYREALTSVGAYDKLFEDFRTFMEAKGLQFNEGRIIDASFVVAPRQRNTREENAKIKEGKGDELWNDNPHKKFHKDVDARWTE
KRGDTFYGYKQHVKVDKGNKVILSYATTPANVHDSKGFEQLLDESDKDKDLYLDAGYAGQESTVKEHGMNPIICEKGRRNHPLTEKQKAENRRKSKTRCL
VEHVFGFEEQSMHGLIVRTIGIVRAKANVAMTNLTYNIFRYIQIVCNKRELAIAQ
Blast result :
Comments
IS1186 , IS1168 and IS1168F are nearly identical: IS1168F vs IS1168, 6 nt (2 aa), IS1168F vs IS1186, 4 nt (2 aa), IS1168 vs IS1186, 9 nt (1 aa). IS1186 was shown to transpose upstream (in several independent strains, at slightly different locations) and to consequently activate the expression of cfiA, a gene coding for a class B, metallo beta-lactamase active against cephamycins and carbapenems (Podglajen et al., 1994).
References
1] Podglajen, I., Breuil, J., and Collatz, E. (1994) . Insertion of a novel DNA sequence, IS1186, upstream of the silent carbapenemase gene cfiA, promotes expression of carbapenem resistance in clinical isolates of bacteroides fragilis. Mol. Microbiol. 12, 105-114.