IS1182
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| L43082 | ND | Staphylococcus aureus | Staphylococcus aureus BM3121 |
DNA section
IS Length : 1864 bp
Ends
IR Length : 14/16
IRL : GGGCTGACCCAAAAGTAGCTCCTATATGAAAAATAGAGGTCTTTCCATTT
IRR : GGGCTGACCCATTAGTCCCTTTTGAACCAAAAAATCGAAGGCACCCGTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGGCTGACCCAAAAGTAGCTCCTATATGAAAAATAGAGGTCTTTCCATTTAGATAGTAGTGGAAAGACCTCTATTTATCTTTTGAAAGTGAATACAAAAA
TAAAGCACACTCTGCTATAATATAGGTGATTAATCCATTTGAGAGAGGTGCTTTATCATGTATCAAAATTATACCATAAACCAACTTTGTTTGCCTATAG
ATCTTGAAATTAAATTAGAAGAAAATGATTTTGCTCATGCCATTGTGCAGTTTGTTGACTCTATTCCCGATGAAGTCTTTCTTCCTTATTATCAATCTAT
GGGTAGACCCCAATATCATCCTCGTATGCTACTTTCCATTATTCTTTGTGCCTATATTCAAGGGGTTTATTCGGGCCGCCAAATACAAAATATGCTGATA
GATAGTATTCGGATGCGCTACTTATCTCAAGAGCAATTCCCTAACTTTCGTACTATTAATCGTTTTCGCGTTCATCCGATTATGAACGATATTCTAGATC
ATTCATTTGTTCAATTTCGTGAGCTACTGGTTCAAAGTGGCTTAATCAGTGGCAGTGCTCTATACATTGATGGTACAAAAATTTAAGCGGATGCGAATAA
ATACTCTTTCGTTTGGAAAAAAAGTATTTTAAAGTATAAGGATAGCCTTGATCAAAAAGCCCTTGAAAACTACCAACAAATGGTGGAAACAAAAATCTTA
CCTGAGTTAATAGATGATTTAAAGGATGAACTTTCTATAGAAGAGATTGAAAGGATTCGTCAATCTTTGGAAGAAAAGGAAAATCAATTAATTGAAGCGA
TTGACCAAACCGAAACAGTAGAAGAACGAAAATTACTTCGTAAAGAAAAATCTGAAGTTCATAAGCAGAAGAAGCGATTCGATGATTTTGCCCAAAGAAA
AATGAGCTACGAAGAACAATTAGTGATTATGGGTGTACGCAATAGTTTCTCAAAGACGGATCATGACGCTACTTTTATGCGAATGAAAGAAGATCACATG
CTTAATGGCCAACTAAAACCAGGTTATAATATTCAACTTGCGACAGAAAATCAATTTGCTCTTGCTTATGATTGTTATCCAAATCCAACAGATACACGAA
CATTTATCCCATTTCTTGAAAAGATTGAATCAAAAATAGGTTTACCAGAAAATATTGTCGCAGATGCCGGTTATGCAAGTGAAGAAAATTATTGTTATGT
GTTAGACGAAACAGAATCCACCCCCATCATTCCGCATCAAGGTTACTTAAAAGAGAAGAAACGCGCCCATAAACAAAATCAGTTTCATCGAGATAATTGG
ACTTACAACGAGTTGGATGATTACTATATTTGTCCCAATCAGAAAAGAGTAGTGTTCTCACATTATTCACAACGTAAAGATAGAAATGGATTTATTCGAA
ACTTCAAAATCTATGAATGTGAAGATTGTACAGGATGTCCATTGAGAGAGAAATGTACCAAAGCAAAAGAAGGAAAAAATCGACGTTTGAATATTAATCC
AACCTGGGAATATATAAAAATAAAAGCGAATGAAAAGATAGAAAGCAAAGAAACAGCCTCATACTATGCGCAGCGAAAGATAGATGTCGAAACAGTTTTT
GGAAACATTAAACAAAATATGAAATTTAAAAGATTCCATGTCCGAGGAGCTGAAAAAATTTTTAAGGAAATGGGACTTGTATTTCTTGCGCACAATTTTA
GGAAATTAGTAACTAGAGTAAGGAAATATGAGGGAAAAACAATCATACAAAACCAAATATAGGGGGAAAATCCTCTATATTTCATCCAAAAAAAACAAAA
ATCGAAGACATCTTTCACGGGTGCCTTCGATTTTTTGGTTCAAAAGGGACTAATGGGTCAGCCC
TAAAGCACACTCTGCTATAATATAGGTGATTAATCCATTTGAGAGAGGTGCTTTATCATGTATCAAAATTATACCATAAACCAACTTTGTTTGCCTATAG
ATCTTGAAATTAAATTAGAAGAAAATGATTTTGCTCATGCCATTGTGCAGTTTGTTGACTCTATTCCCGATGAAGTCTTTCTTCCTTATTATCAATCTAT
GGGTAGACCCCAATATCATCCTCGTATGCTACTTTCCATTATTCTTTGTGCCTATATTCAAGGGGTTTATTCGGGCCGCCAAATACAAAATATGCTGATA
GATAGTATTCGGATGCGCTACTTATCTCAAGAGCAATTCCCTAACTTTCGTACTATTAATCGTTTTCGCGTTCATCCGATTATGAACGATATTCTAGATC
ATTCATTTGTTCAATTTCGTGAGCTACTGGTTCAAAGTGGCTTAATCAGTGGCAGTGCTCTATACATTGATGGTACAAAAATTTAAGCGGATGCGAATAA
ATACTCTTTCGTTTGGAAAAAAAGTATTTTAAAGTATAAGGATAGCCTTGATCAAAAAGCCCTTGAAAACTACCAACAAATGGTGGAAACAAAAATCTTA
CCTGAGTTAATAGATGATTTAAAGGATGAACTTTCTATAGAAGAGATTGAAAGGATTCGTCAATCTTTGGAAGAAAAGGAAAATCAATTAATTGAAGCGA
TTGACCAAACCGAAACAGTAGAAGAACGAAAATTACTTCGTAAAGAAAAATCTGAAGTTCATAAGCAGAAGAAGCGATTCGATGATTTTGCCCAAAGAAA
AATGAGCTACGAAGAACAATTAGTGATTATGGGTGTACGCAATAGTTTCTCAAAGACGGATCATGACGCTACTTTTATGCGAATGAAAGAAGATCACATG
CTTAATGGCCAACTAAAACCAGGTTATAATATTCAACTTGCGACAGAAAATCAATTTGCTCTTGCTTATGATTGTTATCCAAATCCAACAGATACACGAA
CATTTATCCCATTTCTTGAAAAGATTGAATCAAAAATAGGTTTACCAGAAAATATTGTCGCAGATGCCGGTTATGCAAGTGAAGAAAATTATTGTTATGT
GTTAGACGAAACAGAATCCACCCCCATCATTCCGCATCAAGGTTACTTAAAAGAGAAGAAACGCGCCCATAAACAAAATCAGTTTCATCGAGATAATTGG
ACTTACAACGAGTTGGATGATTACTATATTTGTCCCAATCAGAAAAGAGTAGTGTTCTCACATTATTCACAACGTAAAGATAGAAATGGATTTATTCGAA
ACTTCAAAATCTATGAATGTGAAGATTGTACAGGATGTCCATTGAGAGAGAAATGTACCAAAGCAAAAGAAGGAAAAAATCGACGTTTGAATATTAATCC
AACCTGGGAATATATAAAAATAAAAGCGAATGAAAAGATAGAAAGCAAAGAAACAGCCTCATACTATGCGCAGCGAAAGATAGATGTCGAAACAGTTTTT
GGAAACATTAAACAAAATATGAAATTTAAAAGATTCCATGTCCGAGGAGCTGAAAAAATTTTTAAGGAAATGGGACTTGTATTTCTTGCGCACAATTTTA
GGAAATTAGTAACTAGAGTAAGGAAATATGAGGGAAAAACAATCATACAAAACCAAATATAGGGGGAAAATCCTCTATATTTCATCCAAAAAAAACAAAA
ATCGAAGACATCTTTCACGGGTGCCTTCGATTTTTTGGTTCAAAAGGGACTAATGGGTCAGCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1605 bp | 533 aa | 158 | 1762 | + | Yes |
Chemistry : DDE
ORF sequence :
MYQNYTINQLCLPIDLEIKLEENDFAHAIVQFVDSIPDEVFLPYYQSMGRPQYHPRMLLSIILCAYIQGVYSGRQIQNMLIDSIRMRYLSQEQFPNFRTI
NRFRVHPIMNDILDHSFVQFRELLVQSGLISGSALYIDGTKIADANKYSFVWKKSILKYKDSLDQKALENYQQMVETKILPELIDDLKDELSIEEIERIR
QSLEEKENQLIEAIDQTETVEERKLLRKEKSEVHKQKKRFDDFAQRKMSYEEQLVIMGVRNSFSKTDHDATFMRMKEDHMLNGQLKPGYNIQLATENQFA
LAYDCYPNPTDTRTFIPFLEKIESKIGLPENIVADAGYASEENYCYVLDETESTPIIPHQGYLKEKKRAHKQNQFHRDNWTYNELDDYYICPNQKRVVFS
HYSQRKDRNGFIRNFKIYECEDCTGCPLREKCTKAKEGKNRRLNINPTWEYIKIKANEKIESKETASYYAQRKIDVETVFGNIKQNMKFKRFHVRGAEKI
FKEMGLVFLAHNFRKLVTRVRKYEGKTIIQNQI
NRFRVHPIMNDILDHSFVQFRELLVQSGLISGSALYIDGTKIADANKYSFVWKKSILKYKDSLDQKALENYQQMVETKILPELIDDLKDELSIEEIERIR
QSLEEKENQLIEAIDQTETVEERKLLRKEKSEVHKQKKRFDDFAQRKMSYEEQLVIMGVRNSFSKTDHDATFMRMKEDHMLNGQLKPGYNIQLATENQFA
LAYDCYPNPTDTRTFIPFLEKIESKIGLPENIVADAGYASEENYCYVLDETESTPIIPHQGYLKEKKRAHKQNQFHRDNWTYNELDDYYICPNQKRVVFS
HYSQRKDRNGFIRNFKIYECEDCTGCPLREKCTKAKEGKNRRLNINPTWEYIKIKANEKIESKETASYYAQRKIDVETVFGNIKQNMKFKRFHVRGAEKI
FKEMGLVFLAHNFRKLVTRVRKYEGKTIIQNQI
Blast result :
Comments
IS1182 is related to IS1272, as revealed by BLAST. Based on comparison with IS1272, the IS1182 ORF taken into account here is the fusion between the two reported ORFs: 142 aa (158-586) and 391 aa (587-1762), ignoring the stop codon at position 584. An IS1182 copy from Staphylococcus aureus BM3121 has been inactivated by the insertion of IS1181, an ISL3 family member (described in Accession number L43098). Both IS1182 and IS1272 putative transposases display some similarity with two ORF described in pAH4, a plasmid from Acetobacter sp. BPR2001 (Tonouchi et al., 1994 - Accession number D30784). However, detailed comparisons suggest a complex situation, with several possible sequencing errors; therefore, this putative element is not reported here yet.
The sequence containing IS1182 corresponds to the left junction of Tn5405 inserted within Tn5404. This region includes a resL site, one ORF of unknown function and the left copy of IS1182 within Tn5405.
The sequence containing IS1182 corresponds to the left junction of Tn5405 inserted within Tn5404. This region includes a resL site, one ORF of unknown function and the left copy of IS1182 within Tn5405.
References
1] Archer, G.L., Niemeyer, D.M., Thanassi, J.A., and Pucci, M.J. (1994) Antimicrob. Agents Chemother. 38, 447-454.
2] Tonouchi, N., Tsuchida, T., Yoshinaga, F., Horinouchi, S., and Beppu, T. (1994) Biosci. Biotech. Biochem. 58, 1899-1901.
3] Derbise, A., Dyke, K.G.H., and El Solh, N. (1996) "Characterization of a Staphylococcus aureus transposon Tn5405, located within Tn5404 and carrying the aminoglycoside resistance genes, aphA and aadE" EMBL Data Library, Rel. 47.
2] Tonouchi, N., Tsuchida, T., Yoshinaga, F., Horinouchi, S., and Beppu, T. (1994) Biosci. Biotech. Biochem. 58, 1899-1901.
3] Derbise, A., Dyke, K.G.H., and El Solh, N. (1996) "Characterization of a Staphylococcus aureus transposon Tn5405, located within Tn5404 and carrying the aminoglycoside resistance genes, aphA and aadE" EMBL Data Library, Rel. 47.