IS117
- Family IS110
- Group
Isoform Synonym(s) A3(2) mini-circle
Accession number | Transposition | Origin | Host |
---|---|---|---|
X15942 | Y | Streptomyces coelicolor | Streptomyces coelicolor M145 Streptomyces coelicolor A3(2) M130 Streptomyces coelicolor A3(2) J1501 |
DNA section
IS Length : 2527 bp
Ends
Left end : AAGCTTGGTTGGACTTTCACCGTGCGACTCTCGCCGTGAAGTGTTCGGGCCTCAAGGATGATCAATTACGGCTTGCTGCGGCGGCGCCCTCGTCGATGAC
Right end : CGCGTGAGGTACGTCTGCTGCTGCCAGGGTGTGCTCATGAATACGACACCAGATGGACGGCCGATTCCTCCCGCGCATGCCGACGAGCGGACCATGCTGG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GCCCCGATAG | GGTCGGGGNN | 0 | |
CCGCCGATAG | GCGTCGGGNN | 0 | |
CCGCCGATAG | GCGTCGGGNN | 0 | |
CCGCCGATAG | GCGTCGGGNN | 0 | |
GCCGGTCAAG | GGTGAAACNN | 0 | |
GCCGGTCAAG | GGTGAAACNN | 0 | |
GCCGGTCAAG | GGTGAAACNN | 0 | |
GCCCGTCAAG | GTGGAGACNN | 0 | |
GCCCGTCAAG | GTGGAGACNN | 0 | |
CTCCCGATAG | GCACCGGANN | 0 | |
CCTGGTCAAG | GGAAGTGGNN | 0 | |
CACCGGGAAG | AGGTCGGANN | 0 | |
CTCGGTCAAG | GAGTGCGCNN | 0 | |
CGGCGTCAAG | GGACTCGGNN | 0 |
DNA sequence
AAGCTTGGTTGGACTTTCACCGTGCGACTCTCGCCGTGAAGTGTTCGGGCCTCAAGGATGATCAATTACGGCTTGCTGCGGCGGCGCCCTCGTCGATGAC
GCTGCTGGGACTCGTGCAGCACATGGCTGAGGTGGAACGCAACTGGTTCCAGCGTGTGTTCGCAGGTCAGAACGTGCCACCGGTCTTCGGGGAGAGCAAC
CATGATGGCTTTGCCCTGAAGTCTGGACGAGGGCTCGACGAGGCGGTGGCCGCGTGGCAGGCCGAGGTCTCCCGCGGCCGTGAGCTGATCGCTGACGCAG
GACTGGACGACTCCGGCCACCTGTCCGAACAGGAAGCTGGTCACGTCGGCGATCAGGGAGTCTCCCTGCGCTGGATCATGGTGCACATGATCGAGGAATA
CGCACGTCACAACGGTCATGCCGACCTCATCCGCGAGCAGATCGACGGCACCACAGGCGCATGAGGTGTGCTCGCGGAGTCAGCAGGTGACCAGGAACCT
CCGGCCGACTCCAGGTCACGCGGCAGCTTGCAGGAGTGGCGCGAAGGCGCGTTGTTCGTCGAAATGCTGCCGCGTCTGAAGACAGTGGTGGAGTTGGCCG
AGGAACCGGTTGAGCAGGTGGCGCTGGGCTGCGGCGTGCCAGTCTCCGTGTTCGCGTCGGCGCCGGTAGTGCGCGTTGGCCCCGGGCGAGGCCTGTAGGG
CTGCGAAGGCCCAGAGGAAGCCGGCGTTCATGAGGCGGTTGTTCTTGACGAAGCGGCGGCCGACGAAGTGTTTCCTGCCGGAGGCGCGGGTGATCGGTGC
GGAGCCCGCGTAGGACTTCAGGGCTCTGGCGTCGGTGAAGCGGCTGCGGTCGTCGCCGATCTCGGCGAGCACCCGGGCGCCGAGCAAGGGGCCCAGGCCG
GGGAAGCTGAGCAGGATCTCGCTGTCGGCGTGCTCGCGGAACGCGTCTTCGACGGCCTTCGCGAGGTCGTCCGCGGCAAGGCACGTGGCGTCCAGTTGCC
TGAGCAGGGCCAGGAGCTGGTGACCGAAGGCGTCCTCGACGGCCGGAAGCTGGCGGGCGTACTCGGAACGGAAGATGCCCCGCAGCCGCTCGATTTCGGT
GTTGAAGGCGCGAGTGCGGCCGCTGCGCTTCAGCCCGGCGCGCAGCTGGGCGAGGGTGAGCTTCGCGGCCTTCGCCGGGGTTGGCGCCATCGTGAGGATG
ACGCGGGCGTCGGGCCGGGTCAGGCCGCCGTCCTTGCTCTGGAAGGCGTGCAGGGCGGCGGGGTAGTACTCGCGCAGCAGTGAGCGAACCTGGTTGGCGA
CCTGCTGCCGGTTCCAGACCGCGTCCTGCTGAGCGCGGGCCAGCACGGTGATGGCCTGGGCGAGTTCGGAGTCGGCGGGCAGAGGGCGGTGGGCGTGCAT
GTCGGTGCGCAGGATGTTCGCCAGGACCAGGGCGTCGCCGGGGTCGGACTTCTTGCGGGAGACGCCGTGGCGGTCACGGTAGCGGGCGGCCGCGAGCGGA
TTAATTGCGAAGACCTTGCGGCTGCCGGTGCGCAGGGCGGCCACGAGGAGGCCGTGGCTGGTCTCGATGGCCACCGGTATCGGGGTGGCGGAGCTGTCGC
CGTGTTCGGCCAGGAGGTCCAGCAGCTTGTTGTAGCCGGCCACGTCGTCGGTGATGCGGGCCTTGGCGAGCAGGGTGCCGGTGTCGTCGACGATGGCGAC
GTCGTGGTGGCGTTCCGCCCAGTCGATGCCGCAGAACACGGTCAAGCTGTCCTCCCACATTCGATTGTTTATTCAGGTCACGAGCACATGCGGGTCACGC
GGCGCCCTAATCCCAGGACTCGTCGGTCCGCCATCTCAGTAGCCGTTCGCGACACCAGCGCATTTCAGGGGCCTCGATCTGTCTATAGAGCTCTGGGGCT
CGCGATGAACAAGAGGTCGACCCTGGAATGGGCTCGCGCCGTGACGACAACGTCTGGGCGGCCATTGTGAGTTTCGTGGTGGTCACGCAGGCACCGGGCC
GTGCCGCTCTTGACAGAGGCCTGGAACGGCCGGGGGAAACGGAGGCATCAGCCCGGCGCCCGCGTCACCCCACGAAACTCACGAGTACGCGCATGTTCGC
GGTGGTGCGCAGGCTCCGGGCTGTCGCTCTATCGGGAGGCCTCACGGGCCATGGTCGGATCAGACCTTGCCCGGCACCCACGCACCACCGGTCATCGGTG
GGCAGCGGCGCCGGTCTCAGAGAGAGCCTCGGGGACTGGAGAGGGCCGAGGCGTCCGCTGCCCGTGCAAGCGTCTCTTCGCATCCTTGCGGGGTCCGCGA
CGAGCTGCTTACACCCTGGATTAGACATCTCAGGGGGCTAAGAAGTCAAGCGGCCTCCGTCGGCAGAGTCGGCTGTGAGTCATGAGCTGGGAGAGGACAA
GATGTGTGTTCGTCGCCAGCGAAGACGCGCGTGAGGTACGTCTGCTGCTGCCAGGGTGTGCTCATGAATACGACACCAGATGGACGGCCGATTCCTCCCG
CGCATGCCGACGAGCGGACCATGCTGG
GCTGCTGGGACTCGTGCAGCACATGGCTGAGGTGGAACGCAACTGGTTCCAGCGTGTGTTCGCAGGTCAGAACGTGCCACCGGTCTTCGGGGAGAGCAAC
CATGATGGCTTTGCCCTGAAGTCTGGACGAGGGCTCGACGAGGCGGTGGCCGCGTGGCAGGCCGAGGTCTCCCGCGGCCGTGAGCTGATCGCTGACGCAG
GACTGGACGACTCCGGCCACCTGTCCGAACAGGAAGCTGGTCACGTCGGCGATCAGGGAGTCTCCCTGCGCTGGATCATGGTGCACATGATCGAGGAATA
CGCACGTCACAACGGTCATGCCGACCTCATCCGCGAGCAGATCGACGGCACCACAGGCGCATGAGGTGTGCTCGCGGAGTCAGCAGGTGACCAGGAACCT
CCGGCCGACTCCAGGTCACGCGGCAGCTTGCAGGAGTGGCGCGAAGGCGCGTTGTTCGTCGAAATGCTGCCGCGTCTGAAGACAGTGGTGGAGTTGGCCG
AGGAACCGGTTGAGCAGGTGGCGCTGGGCTGCGGCGTGCCAGTCTCCGTGTTCGCGTCGGCGCCGGTAGTGCGCGTTGGCCCCGGGCGAGGCCTGTAGGG
CTGCGAAGGCCCAGAGGAAGCCGGCGTTCATGAGGCGGTTGTTCTTGACGAAGCGGCGGCCGACGAAGTGTTTCCTGCCGGAGGCGCGGGTGATCGGTGC
GGAGCCCGCGTAGGACTTCAGGGCTCTGGCGTCGGTGAAGCGGCTGCGGTCGTCGCCGATCTCGGCGAGCACCCGGGCGCCGAGCAAGGGGCCCAGGCCG
GGGAAGCTGAGCAGGATCTCGCTGTCGGCGTGCTCGCGGAACGCGTCTTCGACGGCCTTCGCGAGGTCGTCCGCGGCAAGGCACGTGGCGTCCAGTTGCC
TGAGCAGGGCCAGGAGCTGGTGACCGAAGGCGTCCTCGACGGCCGGAAGCTGGCGGGCGTACTCGGAACGGAAGATGCCCCGCAGCCGCTCGATTTCGGT
GTTGAAGGCGCGAGTGCGGCCGCTGCGCTTCAGCCCGGCGCGCAGCTGGGCGAGGGTGAGCTTCGCGGCCTTCGCCGGGGTTGGCGCCATCGTGAGGATG
ACGCGGGCGTCGGGCCGGGTCAGGCCGCCGTCCTTGCTCTGGAAGGCGTGCAGGGCGGCGGGGTAGTACTCGCGCAGCAGTGAGCGAACCTGGTTGGCGA
CCTGCTGCCGGTTCCAGACCGCGTCCTGCTGAGCGCGGGCCAGCACGGTGATGGCCTGGGCGAGTTCGGAGTCGGCGGGCAGAGGGCGGTGGGCGTGCAT
GTCGGTGCGCAGGATGTTCGCCAGGACCAGGGCGTCGCCGGGGTCGGACTTCTTGCGGGAGACGCCGTGGCGGTCACGGTAGCGGGCGGCCGCGAGCGGA
TTAATTGCGAAGACCTTGCGGCTGCCGGTGCGCAGGGCGGCCACGAGGAGGCCGTGGCTGGTCTCGATGGCCACCGGTATCGGGGTGGCGGAGCTGTCGC
CGTGTTCGGCCAGGAGGTCCAGCAGCTTGTTGTAGCCGGCCACGTCGTCGGTGATGCGGGCCTTGGCGAGCAGGGTGCCGGTGTCGTCGACGATGGCGAC
GTCGTGGTGGCGTTCCGCCCAGTCGATGCCGCAGAACACGGTCAAGCTGTCCTCCCACATTCGATTGTTTATTCAGGTCACGAGCACATGCGGGTCACGC
GGCGCCCTAATCCCAGGACTCGTCGGTCCGCCATCTCAGTAGCCGTTCGCGACACCAGCGCATTTCAGGGGCCTCGATCTGTCTATAGAGCTCTGGGGCT
CGCGATGAACAAGAGGTCGACCCTGGAATGGGCTCGCGCCGTGACGACAACGTCTGGGCGGCCATTGTGAGTTTCGTGGTGGTCACGCAGGCACCGGGCC
GTGCCGCTCTTGACAGAGGCCTGGAACGGCCGGGGGAAACGGAGGCATCAGCCCGGCGCCCGCGTCACCCCACGAAACTCACGAGTACGCGCATGTTCGC
GGTGGTGCGCAGGCTCCGGGCTGTCGCTCTATCGGGAGGCCTCACGGGCCATGGTCGGATCAGACCTTGCCCGGCACCCACGCACCACCGGTCATCGGTG
GGCAGCGGCGCCGGTCTCAGAGAGAGCCTCGGGGACTGGAGAGGGCCGAGGCGTCCGCTGCCCGTGCAAGCGTCTCTTCGCATCCTTGCGGGGTCCGCGA
CGAGCTGCTTACACCCTGGATTAGACATCTCAGGGGGCTAAGAAGTCAAGCGGCCTCCGTCGGCAGAGTCGGCTGTGAGTCATGAGCTGGGAGAGGACAA
GATGTGTGTTCGTCGCCAGCGAAGACGCGCGTGAGGTACGTCTGCTGCTGCCAGGGTGTGCTCATGAATACGACACCAGATGGACGGCCGATTCCTCCCG
CGCATGCCGACGAGCGGACCATGCTGG
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1245 bp | 414 aa | 565 | 1809 | + | No |
Chemistry : DEDD
ORF sequence :
MWEDSLTVFCGIDWAERHHDVAIVDDTGTLLAKARITDDVAGYNKLLDLLAEHGDSSATPIPVAIETSHGLLVAALRTGSRKVFAINPLAAARYRDRHGV
SRKKSDPGDALVLANILRTDMHAHRPLPADSELAQAITVLARAQQDAVWNRQQVANQVRSLLREYYPAALHAFQSKDGGLTRPDARVILTMAPTPAKAAK
LTLAQLRAGLKRSGRTRAFNTEIERLRGIFRSEYARQLPAVEDAFGHQLLALLRQLDATCLAADDLAKAVEDAFREHADSEILLSFPGLGPLLGARVLAE
IGDDRSRFTDARALKSYAGSAPITRASGRKHFVGRRFVKNNRLMNAGFLWAFAALQASPGANAHYRRRREHGDWHAAAQRHLLNRFLGQLHHCLQTRQHF
DEQRAFAPLLQAAA
SRKKSDPGDALVLANILRTDMHAHRPLPADSELAQAITVLARAQQDAVWNRQQVANQVRSLLREYYPAALHAFQSKDGGLTRPDARVILTMAPTPAKAAK
LTLAQLRAGLKRSGRTRAFNTEIERLRGIFRSEYARQLPAVEDAFGHQLLALLRQLDATCLAADDLAKAVEDAFREHADSEILLSFPGLGPLLGARVLAE
IGDDRSRFTDARALKSYAGSAPITRASGRKHFVGRRFVKNNRLMNAGFLWAFAALQASPGANAHYRRRREHGDWHAAAQRHLLNRFLGQLHHCLQTRQHF
DEQRAFAPLLQAAA
Blast result :
Comments
IS117 is not present in Streptomyces achromogenes ATCC12767, Streptomyces acrimycini IPV1610, Streptomyces albus G, Streptomyces albus P CMI52766, Streptomyces capreolus NCIB9801, Streptomyces clavuligerus ATCC27064, Streptomyces fradiae ATCC10745, Streptomyces glaucescens ETH22794, Streptomyces lipmanii NRRL3584, Streptomyces lividans 66, Streptomyces parvulus ATCC12434, Streptomyces scabies A-26, Streptomyces vinaceus NCIB8852, and Streptomyces violaceolatus. IS117 was discovered as a 2527 bp mini-circle, which actually corresponds to an intermediate in the transposition process. It shows a strongly preferred (20 times better) chromosomal insertion site. IS117 ORF is 24.8, 24.5 and 17 % identical to those of IS900, IS110 and IS492, respectively. No target site duplication occurred on integration onto the preferred or secondary sites. In contrast to integration into the chromosome which typically resulted in tandem insertions, only monomeric insertions were found in the low-copy-number SCP2* (SCP2 contains the IS117 attM site) plasmid. The other two ORF from IS117 (ORF2 and ORF3) are not essential for transposition but may modulate some of its steps (Smokvina et al., 1994). Note that a 360 residues ORF from the pNGR234a plasmid of Rhizobium sp. (Accession Number Z68203 - Freiberg et al., 1996, Genome Res. 6, 590-600) is 31 % identical (in 214 aa overlap) to the IS117 transposase.
References
1] Lydiate, D.J., Ikeda, H., and Hopwood, D.A. (1986) Mol. Gen. Genet. 203, 79-88.
2] Henderson, D.J., Lydiate, D.J., and Hopwood, D.A. (1989) Mol. Microbiol. 3, 1307- 1318
3] Lydiate, D.J., Ashby, A.M., Henderson, D.J., Kieser, H.M., and Hopwood, D.A. (1989) J. Gen. Microbiol. 135, 941-955.
4] Henderson, D.J., Brolle, D.-F., Kieser, T., Melton, R.E., and Hopwood, D.A. (1990) Mol. Gen. Genet. 224, 65-71.
5] Kieser, H.M., Kieser, T., and Hopwood, D.A. (1992) J. Bacteriol. 174, 5496-5507.
6] Smokvina, T., and Hopwood, D.A. (1993) Mol. Gen. Genet. 239, 90-96.
7] Smokvina, T., Henderson, D.J., Melton, R.E., Brolle, D.-F., Kieser, T., and Hopwood, D.A. (1994) Mol. Microbiol. 12, 459-468.
2] Henderson, D.J., Lydiate, D.J., and Hopwood, D.A. (1989) Mol. Microbiol. 3, 1307- 1318
3] Lydiate, D.J., Ashby, A.M., Henderson, D.J., Kieser, H.M., and Hopwood, D.A. (1989) J. Gen. Microbiol. 135, 941-955.
4] Henderson, D.J., Brolle, D.-F., Kieser, T., Melton, R.E., and Hopwood, D.A. (1990) Mol. Gen. Genet. 224, 65-71.
5] Kieser, H.M., Kieser, T., and Hopwood, D.A. (1992) J. Bacteriol. 174, 5496-5507.
6] Smokvina, T., and Hopwood, D.A. (1993) Mol. Gen. Genet. 239, 90-96.
7] Smokvina, T., Henderson, D.J., Melton, R.E., Brolle, D.-F., Kieser, T., and Hopwood, D.A. (1994) Mol. Microbiol. 12, 459-468.