IS116
- Family IS110
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M31716 | Y | Streptomyces clavuligerus | Streptomyces violaceoruber SANK95570 Streptomyces clavuligerus NRRL3585 Streptomyces venezuelae NCIB823 |
DNA section
IS Length : 1421 bp
Ends
Left end : TCCCTAAGAATGTGAGGCGGTCCGGGAGGACCGGGTGTGGCTGATGGTGGTTTGCCATTGATGGCTCCAGGAGAATGGCCGCCCGGTCCTCCGGGATGCT
Right end : CCGCCGACGGGTCGACGTGCTCTGGGCGATGCTGCGTGACAAGAGGCTCTTCACCCCCGCTCCGCCGGTCACACAGACGGCTTGACACGATCATTGAGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATGGTCGG | TCTCCTGGT | 0 |
DNA sequence
TCCCTAAGAATGTGAGGCGGTCCGGGAGGACCGGGTGTGGCTGATGGTGGTTTGCCATTGATGGCTCCAGGAGAATGGCCGCCCGGTCCTCCGGGATGCT
CCGACTGCTGATTGAGAACTGCGGTCATCGCTGGTTAGGGACCTGTCGGCGGGGTGTTCTCCGGGCCACGGAATGCTGGTCACGGTGGTGGAACGGAGCG
AGGCGGTTGAGCACGCGGCATGATCGGATATGGGTCGGCATCGACGCAGGCAAGGGGCATCACTGGGCGGTGGCGGTCGACGCCGACGGGGAGACGTTGT
TCTCGACGAAGGTGATCAACGACGAGGCCCAGGTCCTCACCCTCATCGAGACGGCCCGCGAGCGTGAAGAGGTCCGGTGGGCGGTGGACATCTCCGGGCG
GGCCTCCACGCTGCTGCTGGCCCTGCTGGTCGCCCACGGGCAGAACGTCGTCTACGTGCCCGGCCGCACCGTGAACCGGATGTCCGGGGCTTACAAGGGC
GAGGGAAAGACCGACGCGAAGGACGCGCGGGTCATCGCCGACCAGGCCCGGATGCGCCGGGACTTCGCCCCGCTGGACAGGCCCCCCGAGCTGGTCACTA
CGCTCCGCCTGCTGACGAACCACCGGGCCGACCTGATCGCCGACCGGGTCCGGCTGATCAACCGGCTGCGCGACCTGCTGACCGGCATCTGCCCGGCCCT
GGAGCGGGCCTTCGACTACTCCGCGGCCAAGGGCCCGGTCGTCATGCTGACCGAGTATCAGACCCCCGCCGCCCTGCGGCGGACCGGCGTCAAACGGCTG
ACGACGTGGCTGGGACGCCGCAAGGTCCGCGACGCCGACACCGTCGCCGCCAAGGCGATCGAGGCCGCCCGGACCCAGCAGGTCGTGCTGCCCGGGGAGA
AGCGGGCCACCAAGCTGGTCTGCGACCTTGCTCACCAGCTCCTTGCCCTGGACGAGCGGATCAAGGACAACGACCGCGAGATCCGCGAGACCTTCCGCAC
CGACGACCGCGCCGAGATCATCGAGTCCATGCCCGGCATGGGTCCCGTCCTGGGCGCCGAGTTCGTCGCCATCGTCGGTGACCTGTCCGGCTACAAGGAC
GCCGGCCGACTCGCCTCCCACGCCGGCCTGGCCCCGGTGCCCCGGGATTCCGGCCGCCGCACCGGCAACTATCACCGGCCCCAGCGTTACAACCGGCGCC
TGCGCTGGCTGTTCTACATGTCCGCCCAGACCGCGATGATGCGGCCCGGCCCATCGCGGGACTACTACCTCAAGAAGCGAGGTGAAGGGCTGCTGCACAC
CCAGGCCCTGCTCTCCCTCGCCCGCCGACGGGTCGACGTGCTCTGGGCGATGCTGCGTGACAAGAGGCTCTTCACCCCCGCTCCGCCGGTCACACAGACG
GCTTGACACGATCATTGAGAT
CCGACTGCTGATTGAGAACTGCGGTCATCGCTGGTTAGGGACCTGTCGGCGGGGTGTTCTCCGGGCCACGGAATGCTGGTCACGGTGGTGGAACGGAGCG
AGGCGGTTGAGCACGCGGCATGATCGGATATGGGTCGGCATCGACGCAGGCAAGGGGCATCACTGGGCGGTGGCGGTCGACGCCGACGGGGAGACGTTGT
TCTCGACGAAGGTGATCAACGACGAGGCCCAGGTCCTCACCCTCATCGAGACGGCCCGCGAGCGTGAAGAGGTCCGGTGGGCGGTGGACATCTCCGGGCG
GGCCTCCACGCTGCTGCTGGCCCTGCTGGTCGCCCACGGGCAGAACGTCGTCTACGTGCCCGGCCGCACCGTGAACCGGATGTCCGGGGCTTACAAGGGC
GAGGGAAAGACCGACGCGAAGGACGCGCGGGTCATCGCCGACCAGGCCCGGATGCGCCGGGACTTCGCCCCGCTGGACAGGCCCCCCGAGCTGGTCACTA
CGCTCCGCCTGCTGACGAACCACCGGGCCGACCTGATCGCCGACCGGGTCCGGCTGATCAACCGGCTGCGCGACCTGCTGACCGGCATCTGCCCGGCCCT
GGAGCGGGCCTTCGACTACTCCGCGGCCAAGGGCCCGGTCGTCATGCTGACCGAGTATCAGACCCCCGCCGCCCTGCGGCGGACCGGCGTCAAACGGCTG
ACGACGTGGCTGGGACGCCGCAAGGTCCGCGACGCCGACACCGTCGCCGCCAAGGCGATCGAGGCCGCCCGGACCCAGCAGGTCGTGCTGCCCGGGGAGA
AGCGGGCCACCAAGCTGGTCTGCGACCTTGCTCACCAGCTCCTTGCCCTGGACGAGCGGATCAAGGACAACGACCGCGAGATCCGCGAGACCTTCCGCAC
CGACGACCGCGCCGAGATCATCGAGTCCATGCCCGGCATGGGTCCCGTCCTGGGCGCCGAGTTCGTCGCCATCGTCGGTGACCTGTCCGGCTACAAGGAC
GCCGGCCGACTCGCCTCCCACGCCGGCCTGGCCCCGGTGCCCCGGGATTCCGGCCGCCGCACCGGCAACTATCACCGGCCCCAGCGTTACAACCGGCGCC
TGCGCTGGCTGTTCTACATGTCCGCCCAGACCGCGATGATGCGGCCCGGCCCATCGCGGGACTACTACCTCAAGAAGCGAGGTGAAGGGCTGCTGCACAC
CCAGGCCCTGCTCTCCCTCGCCCGCCGACGGGTCGACGTGCTCTGGGCGATGCTGCGTGACAAGAGGCTCTTCACCCCCGCTCCGCCGGTCACACAGACG
GCTTGACACGATCATTGAGAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1200 bp | 399 aa | 207 | 1406 | + | No |
Chemistry : DEDD
ORF sequence :
LSTRHDRIWVGIDAGKGHHWAVAVDADGETLFSTKVINDEAQVLTLIETAREREEVRWAVDISGRASTLLLALLVAHGQNVVYVPGRTVNRMSGAYKGEG
KTDAKDARVIADQARMRRDFAPLDRPPELVTTLRLLTNHRADLIADRVRLINRLRDLLTGICPALERAFDYSAAKGPVVMLTEYQTPAALRRTGVKRLTT
WLGRRKVRDADTVAAKAIEAARTQQVVLPGEKRATKLVCDLAHQLLALDERIKDNDREIRETFRTDDRAEIIESMPGMGPVLGAEFVAIVGDLSGYKDAG
RLASHAGLAPVPRDSGRRTGNYHRPQRYNRRLRWLFYMSAQTAMMRPGPSRDYYLKKRGEGLLHTQALLSLARRRVDVLWAMLRDKRLFTPAPPVTQTA
KTDAKDARVIADQARMRRDFAPLDRPPELVTTLRLLTNHRADLIADRVRLINRLRDLLTGICPALERAFDYSAAKGPVVMLTEYQTPAALRRTGVKRLTT
WLGRRKVRDADTVAAKAIEAARTQQVVLPGEKRATKLVCDLAHQLLALDERIKDNDREIRETFRTDDRAEIIESMPGMGPVLGAEFVAIVGDLSGYKDAG
RLASHAGLAPVPRDSGRRTGNYHRPQRYNRRLRWLFYMSAQTAMMRPGPSRDYYLKKRGEGLLHTQALLSLARRRVDVLWAMLRDKRLFTPAPPVTQTA
Blast result :
Comments
IS116 is present in Streptomyces clavuligerus NRRL3585 at one copy per genome.
References
1] Leskiw, B.K., Mevarech, M., Barritt, L.S., Jensen, S.E., Henderson, D.J., Hopwood, D.A., Bruton, C.J., and Chater, K.F. (1990) J. Gen. Microbiol. 136, 1251-1258.