IS112
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X56644 | Y | Streptomyces albus | Streptomyces albus G J1147 |
DNA section
IS Length : 883 bp
Ends
IR Length : 16/20
IRL : AGGGCTGTCCCGTAACTGCTGGTCACAGATGAGATGATCTTGGCGTGGCT
IRR : AGGGTTGTCCCGCAAATGATCTTCGGGTCGCCTCGGGTATGGGGGAGGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCCCATTGC | TA | GGGACCGTCT | 2 |
DNA sequence
AGGGCTGTCCCGTAACTGCTGGTCACAGATGAGATGATCTTGGCGTGGCTGGTGTGATCACTGCGTCGGAGCCGTCCTGGATTGCCCCGTTCAGCGGGCT
GAGCCCGAGGCAGTTCGGGAAGCTGGTGACGGTGCTGCGTCGCGAGGGTGCGGACGCGGTCCGCAAGGGCCGGCCGTGGAGCCTGCCCCTGGAGGACCGG
GCACTGCTGGTCGCGGCGTACTGGCGCACAAACCTGACCATGCGGCAGCTCGCCCCGCTGTTCGGGGTGTCGAAGTCGGCGGCGGACCGGATCATCGACC
ACCTCGGACCGATGCTCGCGCTCCAGCCCCGCAAGCGATTCGCCAAGGACACCGTGCTCATCGTGGACGGCACACTGGTCCCCACCCGGGATCACACCAT
CGCCGAGCGGTCGAAGAACTACCGGTACTCCACCAACCACCAGGTCGTCATCGATGCCGACACCCGCCTGGTCGTGGTGGTCGGACGGCCGCTCGCCGGC
AACCGCAACGACTGCAAGGCATGGGAGGAATCCGGCGCAAAGGCCGCCGTCGGCAAGACCCTCACGATCGCCGACGGCGGCTACCCGGGCACCGGACTCG
TCATCCCGCACCGCCGAGAGCGCGGACAGGCCGGACTCCCGGACTGGAAAGAGGAACACAACAAGTCCCACAAGCAGGTCCGAGCCCGCGTCGAGCACGT
CTTCGCCCGCATGAAGACCTGGAAGATCCTCCGCGACTGCCGCCTGAAGGGCGACGGCGTCCATCACGCCATGCTTGGCATCGCCCGCATGCACAACCTC
GCCCTCACCGGATAAACCGGCGGTCCGCACAACGGCCTCCCCCATACCCGAGGCGACCCGAAGATCATTTGCGGGACAACCCT
GAGCCCGAGGCAGTTCGGGAAGCTGGTGACGGTGCTGCGTCGCGAGGGTGCGGACGCGGTCCGCAAGGGCCGGCCGTGGAGCCTGCCCCTGGAGGACCGG
GCACTGCTGGTCGCGGCGTACTGGCGCACAAACCTGACCATGCGGCAGCTCGCCCCGCTGTTCGGGGTGTCGAAGTCGGCGGCGGACCGGATCATCGACC
ACCTCGGACCGATGCTCGCGCTCCAGCCCCGCAAGCGATTCGCCAAGGACACCGTGCTCATCGTGGACGGCACACTGGTCCCCACCCGGGATCACACCAT
CGCCGAGCGGTCGAAGAACTACCGGTACTCCACCAACCACCAGGTCGTCATCGATGCCGACACCCGCCTGGTCGTGGTGGTCGGACGGCCGCTCGCCGGC
AACCGCAACGACTGCAAGGCATGGGAGGAATCCGGCGCAAAGGCCGCCGTCGGCAAGACCCTCACGATCGCCGACGGCGGCTACCCGGGCACCGGACTCG
TCATCCCGCACCGCCGAGAGCGCGGACAGGCCGGACTCCCGGACTGGAAAGAGGAACACAACAAGTCCCACAAGCAGGTCCGAGCCCGCGTCGAGCACGT
CTTCGCCCGCATGAAGACCTGGAAGATCCTCCGCGACTGCCGCCTGAAGGGCGACGGCGTCCATCACGCCATGCTTGGCATCGCCCGCATGCACAACCTC
GCCCTCACCGGATAAACCGGCGGTCCGCACAACGGCCTCCCCCATACCCGAGGCGACCCGAAGATCATTTGCGGGACAACCCT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 771 bp | 256 aa | 45 | 815 | + | No |
Chemistry : DDE
ORF sequence :
MAGVITASEPSWIAPFSGLSPRQFGKLVTVLRREGADAVRKGRPWSLPLEDRALLVAAYWRTNLTMRQLAPLFGVSKSAADRIIDHLGPMLALQPRKRFA
KDTVLIVDGTLVPTRDHTIAERSKNYRYSTNHQVVIDADTRLVVVVGRPLAGNRNDCKAWEESGAKAAVGKTLTIADGGYPGTGLVIPHRRERGQAGLPD
WKEEHNKSHKQVRARVEHVFARMKTWKILRDCRLKGDGVHHAMLGIARMHNLALTG
KDTVLIVDGTLVPTRDHTIAERSKNYRYSTNHQVVIDADTRLVVVVGRPLAGNRNDCKAWEESGAKAAVGKTLTIADGGYPGTGLVIPHRRERGQAGLPD
WKEEHNKSHKQVRARVEHVFARMKTWKILRDCRLKGDGVHHAMLGIARMHNLALTG
Blast result :
Comments
IS112 is responsible for the inactivation of the SalI restriction-modification system of Streptomyces albus G. IS112 is similar to IS493 (29 % identity for the transposases).
References
1] Rodicio, M.R., Alvarez, M.A., and Chater, K.F. (1991) Mol. Gen. Genet. 225, 142- 147.