IS1076
- Family IS3
- Group IS3
Isoform IS1068Synonym(s) IS1076L, IS1076R, Tn5286
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X53013 | ND | Lactococcus lactis | Lactococcus plantarum 1137 Lactococcus lactis subsp. cremoris MG1363 Lactococcus lactis subsp. lactis CNRZ270 plasmid pUCL22 Lactococcus casei 1095 Lactococcus lactis subsp. cremoris C10 Lactococcus lactis subsp. lactis ML3 Lactococcus lactis subsp. lactis biovar diacetylactis D44 Lactococcus lactis subsp. cremoris Lactococcus lactis subsp. lactis 1064 Lactococcus lactis subsp. lactis L32 |
DNA section
IS Length : 1296 bp
Ends
IR Length : 31/39
IRL : TGGAAAGGTTATAATAAACTAGACACAAAGTTAAGAGAAATCGTGGAAAG
IRR : TGGAAAGTCAACGAAAAACTAGACACGGAGTTAAGAGAATTTAAGAATAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNCTCGCGAA | AAA | GAATTTTANN | 3 |
DNA sequence
TGGAAAGGTTATAATAAACTAGACACAAAGTTAAGAGAAATCGTTGGAAAGGTTATAATAAACTAGACACAAAGTTAAGAGAAATCGTGGAAAGGTTTAT
TATGGCAAGAAGAAAATTCGATAAACAATTTAAAAATTCTGCAGTAAAACTCATTCTTGAAGAGGGTTACTCTGTTAAAGAAGTCAGCCAAGAGCTTGAG
GTTCATGCCAATAGTCTTTATCGCTGGGTTCAAGAAGTTGAAGAATATGGAGAAAGTGCTTTTCCAGGCAATGGGACAGCCCTAGCTGATGCCCAACATA
AGATTAAATTGTTAGAGAAAGAAAATCGTTATCTTCAGGAGGAACTTGAACTTCTAAAAAAGTTCCGGGTCCTTCTTGAAGCGAACAGAGTAAAACGTTT
TGAATTTCTCTTGAAACATCATGGGAAGATAAAAATTAAGCATGCAGTAAAAGTTCTTAAGGTTTCTCGCTCAGGTTTCTATGAATACATGCATCGTCGT
CCTTCAAAACAACAAGTGGAGAGAGAAATTCTCTCAGAGAAGATAAAAGCTGTCTTTCATGAGCATAAGGGACGCTATGGTGCGGTTAGAATTACCAAGG
TACTTCATAATACTGGTATTATGACCAACACGAAACGTGTTGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACA
TTACAACAGAAAAAAAGGGGAACGTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGGAGACATG
ACCTATATCCCTACCAAAGAAGGCACCTTATACTTAGCCGTGAATATCGACGTTTTTTCACGTAAGATTGTAGGCTGGTCAATGTCTTCACGGATGCAAG
ATAAACTGGTGAGGGATTGCTTCTTACAAGCTTGTGGGAAAGAACATCCTCAGCCTGGCTTGATTGTCCATACTGATCAAGGGAGTCAATATACCAGCTC
TCGTTATCAATCTACTCTTCGTCAAGTCGGTGCTCAATCTAGCATGAGTCGTAAAGGAAATCCCTATGACAATGCAATGATGGAGTCTTTTTATAAGACG
CTAAAGAGAGAGCTTATTAATGATGCTCATTTTGAGACAAGAGCTGAGGCTACTCAAGAAATATTTAAATACATTGAGACCTATTACAATACAAAATGGA
TGCATTCAGGTCTTGATTACCAGTCGCCAAAAGACTTTGAAAAATATTATTCTTAAATTCTCTTAACTCCGTGTCTAGTTTTTCGTTGACTTTCCA
TATGGCAAGAAGAAAATTCGATAAACAATTTAAAAATTCTGCAGTAAAACTCATTCTTGAAGAGGGTTACTCTGTTAAAGAAGTCAGCCAAGAGCTTGAG
GTTCATGCCAATAGTCTTTATCGCTGGGTTCAAGAAGTTGAAGAATATGGAGAAAGTGCTTTTCCAGGCAATGGGACAGCCCTAGCTGATGCCCAACATA
AGATTAAATTGTTAGAGAAAGAAAATCGTTATCTTCAGGAGGAACTTGAACTTCTAAAAAAGTTCCGGGTCCTTCTTGAAGCGAACAGAGTAAAACGTTT
TGAATTTCTCTTGAAACATCATGGGAAGATAAAAATTAAGCATGCAGTAAAAGTTCTTAAGGTTTCTCGCTCAGGTTTCTATGAATACATGCATCGTCGT
CCTTCAAAACAACAAGTGGAGAGAGAAATTCTCTCAGAGAAGATAAAAGCTGTCTTTCATGAGCATAAGGGACGCTATGGTGCGGTTAGAATTACCAAGG
TACTTCATAATACTGGTATTATGACCAACACGAAACGTGTTGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACA
TTACAACAGAAAAAAAGGGGAACGTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGGAGACATG
ACCTATATCCCTACCAAAGAAGGCACCTTATACTTAGCCGTGAATATCGACGTTTTTTCACGTAAGATTGTAGGCTGGTCAATGTCTTCACGGATGCAAG
ATAAACTGGTGAGGGATTGCTTCTTACAAGCTTGTGGGAAAGAACATCCTCAGCCTGGCTTGATTGTCCATACTGATCAAGGGAGTCAATATACCAGCTC
TCGTTATCAATCTACTCTTCGTCAAGTCGGTGCTCAATCTAGCATGAGTCGTAAAGGAAATCCCTATGACAATGCAATGATGGAGTCTTTTTATAAGACG
CTAAAGAGAGAGCTTATTAATGATGCTCATTTTGAGACAAGAGCTGAGGCTACTCAAGAAATATTTAAATACATTGAGACCTATTACAATACAAAATGGA
TGCATTCAGGTCTTGATTACCAGTCGCCAAAAGACTTTGAAAAATATTATTCTTAAATTCTCTTAACTCCGTGTCTAGTTTTTCGTTGACTTTCCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1170 bp | 389 aa | 87 | 1256 | + | No |
Chemistry : DDE
ORF sequence :
MERFIMARRKFDKQFKNSAVKLILEEGYSVKEVSQELEVHANSLYRWVQEVEEYGESAFPGNGTALADAQHKIKLLEKENRYLQEELELLKKFRVLLEAN
RVKRFEFLLKHHGKIKIKHAVKVLKVSRSGFYEYMHRRPSKQQVEREILSEKIKAVFHEHKGRYGAVRITKVLHNTGIMTNTKRVGKLMHLMGLYAKGSR
YKYKHYNRKKGERSLSRPNLINQIFKATAPNKVWLGDMTYIPTKEGTLYLAVNIDVFSRKIVGWSMSSRMQDKLVRDCFLQACGKEHPQPGLIVHTDQGS
QYTSSRYQSTLRQVGAQSSMSRKGNPYDNAMMESFYKTLKRELINDAHFETRAEATQEIFKYIETYYNTKWMHSGLDYQSPKDFEKYYS
RVKRFEFLLKHHGKIKIKHAVKVLKVSRSGFYEYMHRRPSKQQVEREILSEKIKAVFHEHKGRYGAVRITKVLHNTGIMTNTKRVGKLMHLMGLYAKGSR
YKYKHYNRKKGERSLSRPNLINQIFKATAPNKVWLGDMTYIPTKEGTLYLAVNIDVFSRKIVGWSMSSRMQDKLVRDCFLQACGKEHPQPGLIVHTDQGS
QYTSSRYQSTLRQVGAQSSMSRKGNPYDNAMMESFYKTLKRELINDAHFETRAEATQEIFKYIETYYNTKWMHSGLDYQSPKDFEKYYS
Blast result :
Comments
IS1076 has 24 nt differences with IS904 (including 12 deletions and 1 insertion). IS1076R is flanked by a sequence corresponding to the first 44 bp of IS1076 (insertion within an iso-IS1076 ?). Contrary to IS3, only one long ORF is present in IS1076. The last 300 residues of this ORF are 96 % identical to those of IS1068 ORF2. IS1076L and IS1076R flank two genes involved in a Clp-family proteolytic activity (Huang et al., 1991). Note that a 300-bp fragment displaying 54 % DNA identity with IS1076 is located downstream the alpha-glucosidase gene of Pediococcus pentosaceus PPE1.0 (Leenhouts et al., 1994; Accession Number Z32771). At least 8 copies of IS1076 are present on the 2,560 kb chromosome of the Lactococcus lactis subsp. cremoris reference strain MG1363 (Le Bourgeois et al., 1995).
References