IS1070
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U17353 | ND | Leuconostoc lactis | Leuconostoc lactis NZ6009 plasmid pNZ63 |
DNA section
IS Length : 1027 bp
Ends
IR Length : 24/28
IRL : GGTAGATTGTAAAATTAAACCGAACACTTGAATAAAAAAGCCGCAGCGCC
IRR : GGTAGATTGCAAGAATTAACCGAACACTGTAAACTGCTGAAAAAACTTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTAGATTGTAAAATTAAACCGAACACTTGAATAAAAAAGCCGCAGCGCCTTTAATGGTAATTGACTAAAACAACCATAAAGGAACTGCGACTCATGACT
AGTTTATCATCTCAAGAACGCACTGTCATTCAAACTTTACTCGAATTGAATTATTCTGTTCGTGCCATTGCGCGCTTTATTAAACGCTCTCCTTCAACCG
TTTCGATTGAAATTCATCAAGTTACACCCTATAAAGCTGAGATTGCTCATGCGTTGGCATTGAAAAAACGTCATCTACGTGGTCGTCATGACACGCTAAC
ACCAGCTATCGCTGTCTTTTTGAATCACCACATTGGTATATTGAAGTGGTCACCAGAAACCGCTGCGCATGTTTTAGGGTTACCTTTCAAAACGATTTAT
AATTGGCTCAATGCTGGTCGACTCAAACTATCATTGGCTGATTTACCTGACAAAGGTATCCGTCAAAAGCGTCAATCTGATGGTAGACGACAAGTATTTG
TGCATGGTCGTTCGATTGAAACGAGACCTGAAAGTGTGAAAGCACGACGAACCTTTGGACATTTTGAAGTTGACACCATGCAATCGGGTAAAAGACGTGG
CGATGTGCTGGTGACAATTACTGAAAGACTAAGCCGGCAACATAGCGTGAGACAGGTCACTGGGCGCAATAGTCAGGCAGTGACACCAGTCTTAATTCGT
TTTTTTCAAGGGATCAAAAATGCCAAGTCAATCACTGTTGATCGTGGGCGAGAATTTGCAAGATATAACGAAATCGAGCAAAAACTAGGCATTCCAGTCT
ATTTCGCGCACCCATATTCACCAGAAGAACGTGGGAGTAATGAGATATTAAATCGATACGTCCGTCGTTTTATACCGAAAGAACGCAAAATTGAAACAGT
TAGCGCCAAAGAATTAGATCAAATTAATCATTGGATCAATGCGAGACCTATGAAAACACTCAACTGGCAATCACCACGGAAGTTTTTTCAGCAGTTTACA
GTGTTCGGTTAATTCTTGCAATCTACC
AGTTTATCATCTCAAGAACGCACTGTCATTCAAACTTTACTCGAATTGAATTATTCTGTTCGTGCCATTGCGCGCTTTATTAAACGCTCTCCTTCAACCG
TTTCGATTGAAATTCATCAAGTTACACCCTATAAAGCTGAGATTGCTCATGCGTTGGCATTGAAAAAACGTCATCTACGTGGTCGTCATGACACGCTAAC
ACCAGCTATCGCTGTCTTTTTGAATCACCACATTGGTATATTGAAGTGGTCACCAGAAACCGCTGCGCATGTTTTAGGGTTACCTTTCAAAACGATTTAT
AATTGGCTCAATGCTGGTCGACTCAAACTATCATTGGCTGATTTACCTGACAAAGGTATCCGTCAAAAGCGTCAATCTGATGGTAGACGACAAGTATTTG
TGCATGGTCGTTCGATTGAAACGAGACCTGAAAGTGTGAAAGCACGACGAACCTTTGGACATTTTGAAGTTGACACCATGCAATCGGGTAAAAGACGTGG
CGATGTGCTGGTGACAATTACTGAAAGACTAAGCCGGCAACATAGCGTGAGACAGGTCACTGGGCGCAATAGTCAGGCAGTGACACCAGTCTTAATTCGT
TTTTTTCAAGGGATCAAAAATGCCAAGTCAATCACTGTTGATCGTGGGCGAGAATTTGCAAGATATAACGAAATCGAGCAAAAACTAGGCATTCCAGTCT
ATTTCGCGCACCCATATTCACCAGAAGAACGTGGGAGTAATGAGATATTAAATCGATACGTCCGTCGTTTTATACCGAAAGAACGCAAAATTGAAACAGT
TAGCGCCAAAGAATTAGATCAAATTAATCATTGGATCAATGCGAGACCTATGAAAACACTCAACTGGCAATCACCACGGAAGTTTTTTCAGCAGTTTACA
GTGTTCGGTTAATTCTTGCAATCTACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 95 | 1012 | + | No |
Chemistry : DDE
ORF sequence :
MTSLSSQERTVIQTLLELNYSVRAIARFIKRSPSTVSIEIHQVTPYKAEIAHALALKKRHLRGRHDTLTPAIAVFLNHHIGILKWSPETAAHVLGLPFKT
IYNWLNAGRLKLSLADLPDKGIRQKRQSDGRRQVFVHGRSIETRPESVKARRTFGHFEVDTMQSGKRRGDVLVTITERLSRQHSVRQVTGRNSQAVTPVL
IRFFQGIKNAKSITVDRGREFARYNEIEQKLGIPVYFAHPYSPEERGSNEILNRYVRRFIPKERKIETVSAKELDQINHWINARPMKTLNWQSPRKFFQQ
FTVFG
IYNWLNAGRLKLSLADLPDKGIRQKRQSDGRRQVFVHGRSIETRPESVKARRTFGHFEVDTMQSGKRRGDVLVTITERLSRQHSVRQVTGRNSQAVTPVL
IRFFQGIKNAKSITVDRGREFARYNEIEQKLGIPVYFAHPYSPEERGSNEILNRYVRRFIPKERKIETVSAKELDQINHWINARPMKTLNWQSPRKFFQQ
FTVFG
Blast result :
Comments
The right end contains a putative -35 region (TTGCAA) 6 bp from the tip of the element. Up to ten copies of IS1070 are present in the chromosome of Leuconostoc lactis NZ6009, while five copies are located on the lactose plasmid pNZ63. Two other IS1070-like elements have been partially characterized: IS1070a and IS1070b, and should be considered as iso-IS1070 (only a few substitutions observed). Also, the best interpretation of the sequences flanking the three iso-elements is a DR of 0 bp !
References
1] Vaughan, E.E., and de Vos, W.M. (1995) Gene 155, 95-100.