IS1066

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) IS1067
Accession numberTranspositionOriginHost
U15298 Y Pseudomonas sp.
Pseudomonas sp. P51 plasmid pP51
DNA section
IS Length : 1137 bp

Ends


IR Length : 10

IRL : GTGTTTAATTGCAGTACAAAACGATATTATAATGATGCCAACATCATTTG
IRR : GTGTTTAATTCCGTAAATTGACAATAGTATTTCGAAGCTGAGTGCCCCGT

Insertion site


Left flankDirect repeatRight flankDR Length
NNNTCGCTTCTAGGCGTGANNN2

DNA sequence

GTGTTTAATTGCAGTACAAAACGATATTATAATGATGCCAACATCATTTGACGCAAGGTGGCAGCATGGCGCGGGTGGCGGTGGCGTTGTCGTGTACGGC
GCAAGTCATGGCGGAGCTTGAGAGGTTGTCTCGAAGCCGCAGCGGCGAGGTCCGCATGGCTGAGCGCGCCCGCATCGTGCTGGCTTGCCTGCGCGGCAAA
CGCAACGACGAGATCGCCGACGAAATGGGTTTGCGACCGAATACGGTTGGACAGTGGCGCCGCCGCTTTGCCCAGCGTGGTATCGCAGGGCTTCATGATG
CGCCCAGGTCCGGCAAGCCACCCAAGTACGGCGTGGAGTTGCGCGACCGAATCCTGGCGCAGCTCGAGTTGCCGCCGCCCGAGGGCATGGCGAGTTGGGA
TGGTGGCTCGTTGGCGATGGCGCTGAGCGTGTCCGACGACGCGGTGTGGCGCGCGCTGCGCAAAGAAGGCATCCAGTTGCAGCGCCACCGATCATGGTGC
GTGAGTACGGACCCAGAGTTCGCGGCCAAGGCCGCCGACGTGATCGGCCTGTACTTGAACCCGCCACAGAACGCCTTGGTGCTCAGCGTTGACGAGAAGC
CGTCGATCCAGGCGCTGGAGCGCGCGCGAGGCTACGTGCAGACCAGCAGCGGCAAGATCGTCCAGGGCATGAAGAGCACCTACAAGCGCCACGGCACGGT
CAACCTGTTCGCCGCGCTGGAGGTTGCCACGGGCATCATCCGCGGCAAGACCACCCAGACCAAGAAGCGGGCCGACTTTCAGGCCTTCATGGACGAGGTC
GTTGCCGACCAACCCGCTGACCGGCAAATCCACGTCATCCTGGACAACTTGAGCACCCACAAGAAAAACGAGGACTGGCTCGCCGCGCATCCGAACGTCA
CCTTTCACTTCACGCCCACCAGCGCCAGCTGGCTCAACCAGGTTGAGATCTGGTTTGGCATCTTCCAGCGAAAAACGCTCAACAACGCGAGCTTCCAAAG
CACCGAGCACCTCGTCGCCGCCATCCACGCGTTCACTGCCGCGTACAACGAGAACGCCGCACCTTTTGTCTGGCGCAAACGAGAAGTACGGGGCACTCAG
CTTCGAAATACTATTGTCAATTTACGGAATTAAACAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1068 bp355 aa661133+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARVAVALSCTAQVMAELERLSRSRSGEVRMAERARIVLACLRGKRNDEIADEMGLRPNTVGQWRRRFAQRGIAGLHDAPRSGKPPKYGVELRDRILAQL
ELPPPEGMASWDGGSLAMALSVSDDAVWRALRKEGIQLQRHRSWCVSTDPEFAAKAADVIGLYLNPPQNALVLSVDEKPSIQALERARGYVQTSSGKIVQ
GMKSTYKRHGTVNLFAALEVATGIIRGKTTQTKKRADFQAFMDEVVADQPADRQIHVILDNLSTHKKNEDWLAAHPNVTFHFTPTSASWLNQVEIWFGIF
QRKTLNNASFQSTEHLVAAIHAFTAAYNENAAPFVWRKREVRGTQLRNTIVNLRN

 

Blast result :
Comments
One nt different with IS1067. Tn5280 is a composite transposon [IS1066 - IS1067] carrying the chlorobenzene dioxygenase tcbAB gene cluster. Considering the observations made on other IS630-related elements, the limits of IS1066 and IS1067 have been reconsidered: the TA is considered as the DRs. All IS1066 and IS1067 coordinates have been changed accordingly.
References
1] van der Meer, J.R., Zehnder, A.J.B., and de Vos, W.M. (1991) J. Bacteriol. 173, 7077-7083.
2] Fournier, P., Paulus, F., and Otten, L. (1993) J. Bacteriol. 175, 3151-3160.