ISPfu5
- Family IS6
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003413 | ND | Pyrococcus furiosus | Pyrococcus furiosus |
DNA section
IS Length : 779 bp
Ends
IR Length : 15/16
IRL : GACACTGTCAGGATAAGCAGTGGGACGTTAGGTTTAAGTTTCTGATACCT
IRR : GATACTGTCAGGATAACTGGGGTATCACCTCTTGAAGCCATTCAGTCACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTTCCTGTT | CTCGGGAGA | ACAGGAGCTG | 9 |
| TCCTTCGATA | TCTTCCGAG | CTTGGGTGCTT | 9 |
| TGTTTCTAAA | GTCGATCAA | ATTAGGAATA | 9 |
| AGTGTCGATG | AGGGCAAGC | AAAGGCAAC | 9 |
DNA sequence
GACACTGTCAGGATAAGCAGTGGGACGTTAGGTTTAAGTTTCTGATACCTCTTCAGAAAAGGGGAGAGAAATGAGGACTGAAACCATTATCTACTTACTG
GTTTCAGTCTTAAAAACCTTTCGCCGGAACAAAATCCCAGCAAAAAAGAAAACCAGGGCAATAAACCTGTACCTGCACGGACTAAGTTACAGACAGGTAG
GAACAATCCTCGAAATCAGCCACACAACAGTCTGGGAAACAGTCCAAAAATTCGCGAAAGCAGTTTACCAGCCGAAAATCCTCGCAGTCAAAAAACAGAG
AAACTTCATCGCAATTGACGAGACAGTGATAAAGATCAACGGCCAGAAGAGATTTCTCTGGGCTGCAATCGACGTTGAGAGCAAAGAAATCCTAGCAGTA
TGGATTACAAGCGTTAGGAACTGGTGGATTGCCAGGGACTTCATTCTAGTTGTTTTGAAATCCTGCGAGGGACAGCCAATTTTCCTGGTTGACAAAGGGC
CGTGGTATAAATCAGCGTTTAAATCTCTCGGGCTGGATTATCTGCATGTGACTTTCGGGCCGAGGAACTGTGTTGAGCGCTGGTTTAGGACTGTTAAAGA
GAGAACAAAGCGTTTCTGGAATAACTTCAGGGCTAGAGACTGGAGGAGGGTTCACAGGTTTGTTTTTCTGTTTTCATTCTGGTATAATTTTGTTAGAATT
CATTCTCGGTTTGGTGGACCGCCTGGTGATGTGACTGAATGGCTTCAAGAGGTGATACCCCAGTTATCCTGACAGTATC
GTTTCAGTCTTAAAAACCTTTCGCCGGAACAAAATCCCAGCAAAAAAGAAAACCAGGGCAATAAACCTGTACCTGCACGGACTAAGTTACAGACAGGTAG
GAACAATCCTCGAAATCAGCCACACAACAGTCTGGGAAACAGTCCAAAAATTCGCGAAAGCAGTTTACCAGCCGAAAATCCTCGCAGTCAAAAAACAGAG
AAACTTCATCGCAATTGACGAGACAGTGATAAAGATCAACGGCCAGAAGAGATTTCTCTGGGCTGCAATCGACGTTGAGAGCAAAGAAATCCTAGCAGTA
TGGATTACAAGCGTTAGGAACTGGTGGATTGCCAGGGACTTCATTCTAGTTGTTTTGAAATCCTGCGAGGGACAGCCAATTTTCCTGGTTGACAAAGGGC
CGTGGTATAAATCAGCGTTTAAATCTCTCGGGCTGGATTATCTGCATGTGACTTTCGGGCCGAGGAACTGTGTTGAGCGCTGGTTTAGGACTGTTAAAGA
GAGAACAAAGCGTTTCTGGAATAACTTCAGGGCTAGAGACTGGAGGAGGGTTCACAGGTTTGTTTTTCTGTTTTCATTCTGGTATAATTTTGTTAGAATT
CATTCTCGGTTTGGTGGACCGCCTGGTGATGTGACTGAATGGCTTCAAGAGGTGATACCCCAGTTATCCTGACAGTATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 702 bp | 233 aa | 71 | 772 | + | No |
Chemistry : DDE
ORF sequence :
MRTETIIYLLVSVLKTFRRNKIPAKKKTRAINLYLHGLSYRQVGTILEISHTTVWETVQKFAKAVYQPKILAVKKQRNFIAIDETVIKINGQKRFLWAAI
DVESKEILAVWITSVRNWWIARDFILVVLKSCEGQPIFLVDKGPWYKSAFKSLGLDYLHVTFGPRNCVERWFRTVKERTKRFWNNFRARDWRRVHRFVFL
FSFWYNFVRIHSRFGGPPGDVTEWLQEVIPQLS
DVESKEILAVWITSVRNWWIARDFILVVLKSCEGQPIFLVDKGPWYKSAFKSLGLDYLHVTFGPRNCVERWFRTVKERTKRFWNNFRARDWRRVHRFVFL
FSFWYNFVRIHSRFGGPPGDVTEWLQEVIPQLS
Blast result :
Comments
ISPfu5 is 92% aa similar to ISPfu1 and ISPfu2.
References
1] Diruggiero J, Dunn D, Maeder DL, Holley-Shanks R, Chatard J, Horlacher R, Robb FT, Boos W, Weiss RB. Evidence of recent lateral gene transfer among hyperthermophilic archaea. Mol Microbiol. 2000 Nov;38(4):684-93.
2] Wirojne Kanoksilapatham, Juan M. Gonzalez, Dennis L. Maeder, Jocelyne DiRuggiero, Frank T. Robb. A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei. Archaea. 2004. Aug; 1: 275-281.
2] Wirojne Kanoksilapatham, Juan M. Gonzalez, Dennis L. Maeder, Jocelyne DiRuggiero, Frank T. Robb. A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei. Archaea. 2004. Aug; 1: 275-281.