ISPfu1
- Family IS6
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003413 | Y | Pyrococcus woesei | Pyrococcus furiosus Pyrococcus woesei Thermococcus litoralis |
DNA section
IS Length : 781 bp
Ends
IR Length : 15/16
IRL : GATACTATTAGGATAAGCTGTGGGGTGTCAGGTTTAAGTTACTGGCAGTA
IRR : GATACTGTTAGGATAACTGGGGCATCACCTCCTGAAGCCATTCAGTAACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCAGTATGG | GTTGAGGA | AGTCTAGAGT | 8 |
| TCTTTCTTAT | TGCTTTTG | ATACGGTGAA | 8 |
| AAAAAGCAAT | GTGGAAGC | ATTCATAAAA | 8 |
| CGAAAATTGT | CTTTTTCA | ACACAAAAAC | 8 |
DNA sequence
GATACTATTAGGATAAGCTGTGGGGTGTCAGGTTTAAGTTACTGGCAGTACTTTAGAAAAAGAAGGGGGAACATGAAGTCTGAAACCATTATTTACTGGG
TGGTTTCAGCCTTAAAACCCTTTCGTCGCAACAAAATCCCACCAGAAAAGAAAATCAGGGGAGTAGAATTATACCTGCGAGGCCTCAGTTACCGGCAAAC
CGCCAGAATACTCAAAATCAGTCACGTAACAGTCTGGGAGGCAGTCCAAAAACTCGCAGAAGCAGTTTACAAGCCAAAAATCCTCGCAGTCAAAAAACAG
CGAAACTTCATCGCAGTTGACGAAACAGTAATAAAAATCAACGGGAAGAAAAGATACCTCTGGGCTGCAATTGACGTTGAGAGCAAGGAAGTTTTAGCAG
TCTGGATTACGACTGTTAGAAACTGGTGGGTTGCCAGGGATTTCATTCTGGTTGTTTTAAAGTCGTGTGAAGGGCAGCCTGTCTTTCTGGTTGACAGGGC
GAGCTGGTATAAGTCTGCTTTTAAGAGTTTGAGGTTGGGTTATCTGCATGTGACTTTCGGGCCGAGGAACAGTGTTGAGCGCTGGTTTAGGACGTTGAAG
GAGAGGACGAAGCGTTTCTGGAATAATTTCAGGGGTAAAGACTGGAGGAGGGTTCATAGGTTTGTTTTTCTGTTTGCCTTCTGGTATAATTTTGTCAGAA
TTCATTCTAGTTTTGGTGATCCGCCTGGTGATGTTACTGAATGGCTTCAGGAGGTGATGCCCCAGTTATCCTAACAGTATC
TGGTTTCAGCCTTAAAACCCTTTCGTCGCAACAAAATCCCACCAGAAAAGAAAATCAGGGGAGTAGAATTATACCTGCGAGGCCTCAGTTACCGGCAAAC
CGCCAGAATACTCAAAATCAGTCACGTAACAGTCTGGGAGGCAGTCCAAAAACTCGCAGAAGCAGTTTACAAGCCAAAAATCCTCGCAGTCAAAAAACAG
CGAAACTTCATCGCAGTTGACGAAACAGTAATAAAAATCAACGGGAAGAAAAGATACCTCTGGGCTGCAATTGACGTTGAGAGCAAGGAAGTTTTAGCAG
TCTGGATTACGACTGTTAGAAACTGGTGGGTTGCCAGGGATTTCATTCTGGTTGTTTTAAAGTCGTGTGAAGGGCAGCCTGTCTTTCTGGTTGACAGGGC
GAGCTGGTATAAGTCTGCTTTTAAGAGTTTGAGGTTGGGTTATCTGCATGTGACTTTCGGGCCGAGGAACAGTGTTGAGCGCTGGTTTAGGACGTTGAAG
GAGAGGACGAAGCGTTTCTGGAATAATTTCAGGGGTAAAGACTGGAGGAGGGTTCATAGGTTTGTTTTTCTGTTTGCCTTCTGGTATAATTTTGTCAGAA
TTCATTCTAGTTTTGGTGATCCGCCTGGTGATGTTACTGAATGGCTTCAGGAGGTGATGCCCCAGTTATCCTAACAGTATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 702 bp | 233 aa | 73 | 774 | + | No |
Chemistry : DDE
ORF sequence :
MKSETIIYWVVSALKPFRRNKIPPEKKIRGVELYLRGLSYRQTARILKISHVTVWEAVQKLAEAVYKPKILAVKKQRNFIAVDETVIKINGKKRYLWAAI
DVESKEVLAVWITTVRNWWVARDFILVVLKSCEGQPVFLVDRASWYKSAFKSLRLGYLHVTFGPRNSVERWFRTLKERTKRFWNNFRGKDWRRVHRFVFL
FAFWYNFVRIHSSFGDPPGDVTEWLQEVMPQLS
DVESKEVLAVWITTVRNWWVARDFILVVLKSCEGQPVFLVDRASWYKSAFKSLRLGYLHVTFGPRNSVERWFRTLKERTKRFWNNFRGKDWRRVHRFVFL
FAFWYNFVRIHSSFGDPPGDVTEWLQEVMPQLS
Blast result :
Comments
The insertion sites currently appear to be random and do not retain memmory of prior locations.
ISPfu1 is 92% aa similar to ISPfu2 and 50% aa to IS1216.
There are 8 copies in the Pyrococcus furiousus genome.
ISPfu1 is 92% aa similar to ISPfu2 and 50% aa to IS1216.
There are 8 copies in the Pyrococcus furiousus genome.
References
1] Diruggiero J, Dunn D, Maeder DL, Holley-Shanks R, Chatard J, Horlacher R, Robb FT, Boos W, Weiss RB. Evidence of recent lateral gene transfer among hyperthermophilic archaea. Mol Microbiol. 2000 Nov;38(4):684-93.
2] Wirojne Kanoksilapatham, Juan M. Gonzalez, Dennis L. Maeder, Jocelyne DiRuggiero, Frank T. Robb. A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei. Archaea. 2004. Aug; 1: 275-281.
2] Wirojne Kanoksilapatham, Juan M. Gonzalez, Dennis L. Maeder, Jocelyne DiRuggiero, Frank T. Robb. A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei. Archaea. 2004. Aug; 1: 275-281.