ISOur1
- Family IS6
- Group
Isoform IS1008Synonym(s) IS1009
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY177427 | ND | Oligella urethralis | Acinetobacter baumannii Acinetobacter baumannii WH8144 plasmid pWH8144 Oligella urethralis |
DNA section
IS Length : 820 bp
Ends
IR Length : 17
IRL : GGCACTGTTGCAAATAGGCTGACATGATAAGCTAAATATCTTATTTATTT
IRR : GGCACTGTTGCAAATAGAGATTTGAGTCGATGATGTTCCCTTTAAAAAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACAAAAATC | ACGAATGGGC | 0 |
DNA sequence
GGCACTGTTGCAAATAGGCTGACATGATAAGCTAAATATCTTATTTATTTCGAGATACAGCAGATGAATCCCTTCCATGGTCGGCACTTTCAAGGTGAAA
TCATTCTTTGGGCTGTTCGTTGGTATTGTAAATATGGCATCAGCTATCGTGAACTTCAAGAAATGCTCGCTGAACGAGGTGTGAATGTTGACCACACCAC
AATTTATCGTTGGGTTCAGCGTTATGCTCCAGAAATGGAAAAACGGTTACGCTGGTATTGGCGTAATCCTACAGATTTACATTCATGGCATATGGATGAG
ACTTATATCAAAGTGAAGGGGCGATGGACTTACCTGTATCGTGCAGTTGATCAACGGGGTCATACGATTGACTTTTACCTTTCCGCTAGACGGAACAGTA
AATCAGCCTATAGTTTTCTAGGAAAGATCTTCAATACGGTGAAAAAATGGCAAATTCCACGGGTCATCAATACAGATAAAGCAGCGACCTATGGCCATGC
TTTATCACGATTAAAGCGAGAAGGAAAATGTCCAGTAGATATTGAGCACAGGCAGATTAAGTATAAAAATAATGTCATTGAATGTGATCATGGTAAGTTA
AAGCGAATCATCAGGGCCACATTAGGATTCAAATCTATGAAGACTGCTTATGCCACAATTAAAGGTATTGAAGTCATGCGTGCACTACGTAAAGGACAAG
CATCGTCATTTTATTATGGTCAGCCTCAGGGTGAAGTGTGTCTAATCAACAGGGTTTTCGGTCTCTAAGTACTTTTTAAAGGGAACATCATCGACTCAAA
TCTCTATTTGCAACAGTGCC
TCATTCTTTGGGCTGTTCGTTGGTATTGTAAATATGGCATCAGCTATCGTGAACTTCAAGAAATGCTCGCTGAACGAGGTGTGAATGTTGACCACACCAC
AATTTATCGTTGGGTTCAGCGTTATGCTCCAGAAATGGAAAAACGGTTACGCTGGTATTGGCGTAATCCTACAGATTTACATTCATGGCATATGGATGAG
ACTTATATCAAAGTGAAGGGGCGATGGACTTACCTGTATCGTGCAGTTGATCAACGGGGTCATACGATTGACTTTTACCTTTCCGCTAGACGGAACAGTA
AATCAGCCTATAGTTTTCTAGGAAAGATCTTCAATACGGTGAAAAAATGGCAAATTCCACGGGTCATCAATACAGATAAAGCAGCGACCTATGGCCATGC
TTTATCACGATTAAAGCGAGAAGGAAAATGTCCAGTAGATATTGAGCACAGGCAGATTAAGTATAAAAATAATGTCATTGAATGTGATCATGGTAAGTTA
AAGCGAATCATCAGGGCCACATTAGGATTCAAATCTATGAAGACTGCTTATGCCACAATTAAAGGTATTGAAGTCATGCGTGCACTACGTAAAGGACAAG
CATCGTCATTTTATTATGGTCAGCCTCAGGGTGAAGTGTGTCTAATCAACAGGGTTTTCGGTCTCTAAGTACTTTTTAAAGGGAACATCATCGACTCAAA
TCTCTATTTGCAACAGTGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 705 bp | 234 aa | 64 | 768 | + | No |
Chemistry : DDE
ORF sequence :
MNPFHGRHFQGEIILWAVRWYCKYGISYRELQEMLAERGVNVDHTTIYRWVQRYAPEMEKRLRWYWRNPTDLHSWHMDETYIKVKGRWTYLYRAVDQRGH
TIDFYLSARRNSKSAYSFLGKIFNTVKKWQIPRVINTDKAATYGHALSRLKREGKCPVDIEHRQIKYKNNVIECDHGKLKRIIRATLGFKSMKTAYATIK
GIEVMRALRKGQASSFYYGQPQGEVCLINRVFGL
TIDFYLSARRNSKSAYSFLGKIFNTVKKWQIPRVINTDKAATYGHALSRLKREGKCPVDIEHRQIKYKNNVIECDHGKLKRIIRATLGFKSMKTAYATIK
GIEVMRALRKGQASSFYYGQPQGEVCLINRVFGL
Blast result :
Comments
ISOur1 is 96% aa simialr to IS1006.
ISOur1 was identified immediately upstream of blaABA-1, providing a -35 promoter sequence (TTGCAA)that may constitute a hybrid promoter together with a putative -10 promoter region (TATAAA) and likely giving rise to a hybrid promoter region. The -35 and -10 regions of this promoter were separated by a 17-bp sequence.
File updated: September 30 2011.
ISOur1 was identified immediately upstream of blaABA-1, providing a -35 promoter sequence (TTGCAA)that may constitute a hybrid promoter together with a putative -10 promoter region (TATAAA) and likely giving rise to a hybrid promoter region. The -35 and -10 regions of this promoter were separated by a 17-bp sequence.
File updated: September 30 2011.
References
1] Mammeri,H., Poirel,L., Mangeney,N. and Nordmann,P.(2003) Antimicrob. Agents Chemother. 47 (5), 1536-1542
2]Weiyuan Wu (2011)
3]Yiqi Fu (2011)
2]Weiyuan Wu (2011)
3]Yiqi Fu (2011)