ISEcl1

  • Family IS3
  • Group IS2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF342826 ND Enterobacter cloacae
Enterobacter cloacae cloacae 13047
Enterobacter cloacae plasmid pPDL12
Enterobacter cloacae cloacae 13047p plasmid pECL A
DNA section
IS Length : 1336 bp

Ends


IR Length : 30/47

IRL : TGATGTAGCCCCCTGAAACACCGGACAGTAGCTGTATCTCCAGATAAGAG
IRR : TGATCTTGACCCTCCATCTCCGAACAGCTTTTGTATCTTAAGTTAACGAT

Insertion site


Left flankDirect repeatRight flankDR Length
TATTGCCGTATTTGGCGCTATTGCTTG2
CTACCTGTTAGGGCTGAAAGGCAGAATAGGCAGCG15
AGAGATGGCAACGATGTTTTGTTGTTCTCAAAAGGA16
TGATGTATTTTGCCAAACAGCTTACCAAGAACGCC15
GTTGAAGTAAATGCCCTTGCTTCCT5
CGCGCTTGTATTCAACATCGAAATTGAGTGGTAAAG16
CTTTTTCTGACGGAAGTGCGCGGTACGTTCCCAGCA16
TTCAAGGAAAGTTTTTAGCAATTCCGTATCCACATC16
GGAGCGCTATGCCACCGGGATTAGCGAAATAGTCA15
TCGTAACATGGTTCCATGTTATCACTGGGTTATCA15
ACTGATTTCCCCATGCATCGAAACTGTTAGAAGTT15
CTGAATTTGATCGAAAGCGTCCATAGCTTCTTTCAT16
ATGAAATCATCTGCGCTGACCTGTCGCTGAACAAC15

DNA sequence

TGATGTAGCCCCCTGAAACACCGGACAGTAGCTGTATCTCCAGATAAGAGATAGGCTTGAATATATGTCTAACACTAACGCCAATTTTGAGATGACCGGG
ATCCTGTTAGGGCAAGAAGTCCGTAAACGTAAAACTCCTCAGGAGAAGATCGCCATTATCCAGCAGACGATGGAGCCGGGTATGAATGTCTCCCATGTCG
CCCGCCTGCATGGTATCCAGCCCAGCCTGCTGTTTAAGTGGAAGAAGCAATATCAGGAAGGCAGCCTCACCGCCGTTGCGGCTGGAGAGGAAGTCGTTCC
TGCTTCTGAGCTTACTGCTGCTCTGAAGCAGGTCCGGGAGCTTCAGCGCCTTCTGGGCAAGAAAACGATGGAAGTTGAGATCCTGAAAGAAGCCGTGGAG
TACGGTCAGTCGCGAAAATGGATAGCGCACGCGCCCTTGTTGCCAAAGGACGGGGAATAGCCATGGTCAGCCGGACCATGGGCGTGTCGCGTGCGCAACT
GTCACTGCGGATTAACCGTTCTGCCGACTGGCAGGACAGGCGCTGTAACCGGCGTAATGAAGAAGCAGACGCAGTAATACTGTCGGCTATCCTCAACATT
ATCAGCGATATGCCGAGTTATGGTTATCGACGCGTGTGGGGCATCCTGCGCAAGCAACGTCGCACAGAGGGACAGCCACCTGTGAATGCCAAACGGCTTT
ACAGGATAATGAGCGAGCATAACCTGTTGTTGTTGCATCACAAACCAGAGCGACCGAAGCGTGAACATAAGGGCAAGATAGCGGTGGCAGAAAGCGATAT
GCGCTGGTGTTCAGATGGCTTCGAGTTCGGCTGCGACAACGGCGAAAAACTGCGGGTAACGTTCGCGCTGGACTGCTGCGACCGTGAGGCCATAGACTGG
GCAGCAAGCACGGGAGGCTATGACAGTTCGACCGTGCAGGATGTGATGCTGAGGTCGGTGGAAAAGCGCTTCGGCGACAGGTTGCCCGACACAGCGGTGC
AGTGGCTGACGGATAACGGTTCAGCGTATACCGCGTATGAAACGCGGAGGTTCGCGAGAGAGCTGAATCTGGAGCCCTGCACAACAGCGGTGAGCAGCCC
GCAGAGTAATGGCATGGCCGAACGGTTCGTGAAAACGATGAAAGAAGACTATATCGCGTTCATGCCAAAACCGGATGTGAGAACAGCACTGCGAAACCTT
GCAGTAGCGTTCACGCATTACAATGAAAACCACCCGCACAGCGCGCTGGGATATCACTCCCCGAGGGAATACCGGCGGCAGCGGACATCGTTAACTTAAG
ATACAAAAGCTGTTCGGAGATGGAGGGTCAAGATCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
369 bp122 aa92460+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTGILLGQEVRKRKTPQEKIAIIQQTMEPGMNVSHVARLHGIQPSLLFKWKKQYQEGSLTAVAAGEEVVPASELTAALKQVRELQRLLGKKTMEVEILKE
AVEYGQSRKWIAHAPLLPKDGE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
921 bp306 aa3791299+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

DPERSRGVRSVAKMDSARALVAKGRGIAMVSRTMGVSRAQLSLRINRSADWQDRRCNRRNEEADAVILSAILNIISDMPSYGYRRVWGILRKQRRTEGQP
PVNAKRLYRIMSEHNLLLLHHKPERPKREHKGKIAVAESDMRWCSDGFEFGCDNGEKLRVTFALDCCDREAIDWAASTGGYDSSTVQDVMLRSVEKRFGD
RLPDTAVQWLTDNGSAYTAYETRRFARELNLEPCTTAVSSPQSNGMAERFVKTMKEDYIAFMPKPDVRTALRNLAVAFTHYNENHPHSALGYHSPREYRR
QRTSLT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1208 bp402 aa921299+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTGILLGQEVRKRKTPQEKIAIIQQTMEPGMNVSHVARLHGIQPSLLFKWKKQYQEGSLTAVAAGEEVVPASELTAALKQVRELQRLLGKKTMEVEILKE
AVEYGQSRKWIAHAPLLPKGRGIAMVSRTMGVSRAQLSLRINRSADWQDRRCNRRNEEADAVILSAILNIISDMPSYGYRRVWGILRKQRRTEGQPPVNA
KRLYRIMSEHNLLLLHHKPERPKREHKGKIAVAESDMRWCSDGFEFGCDNGEKLRVTFALDCCDREAIDWAASTGGYDSSTVQDVMLRSVEKRFGDRLPD
TAVQWLTDNGSAYTAYETRRFARELNLEPCTTAVSSPQSNGMAERFVKTMKEDYIAFMPKPDVRTALRNLAVAFTHYNENHPHSALGYHSPREYRRQRTS
LT

 

Blast result :
Comments
ISEcl1 is 88% (ORF1) and 77% (ORF2) aa similar to IS2.
File updated : January 27 2012.
References
1] Eaton,R.W. and Karns,J.S. (1991) J. Bacteriol. 173 (3), 1363-1366
2] Karns,J.S. and Eaton,R.W. (1997) J. Agric. Food Chem. 45, 1017-1022