ISPst9

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AJ582631 ND Pseudomonas stutzeri
Pseudomonas stutzeri AN142
DNA section
IS Length : 2472 bp

Ends


IR Length : 24

IRL : GGGTATGCGGATTTAATGGTTGATATGTGAAGTAGAAAGCGGAAGGGAAA
IRR : GGGTATGCGGATTTAATGGTTGATGGGGCACCTTCCCCTCACCAACCTGA

Insertion site


Left flankDirect repeatRight flankDR Length
ACCGAAGTTGACAAATTCTCCGT GGTGC8

DNA sequence

GGGTATGCGGATTTAATGGTTGATATGTGAAGTAGAAAGCGGAAGGGAAAACTAAATCCACAACGGACTAAATGGTTGATAGCTAACCGTGCCGGTCTTT
CCAGATCCCCTTCCCTATAGCCATATGTAGGGAACACTTGAAAACTAGGCATATCTGTCTTGACAATAGCCCCGGTACAAGAAGCGGGTGTCGTTGTGCT
CAGCCAGCCTTTCTTCGCCAAAAGCAATATTGCCACGAAGGGTGGTAGCCTACGGGCTCAGTACCTCCCCAGTTGGGTCATCGCCCGGCTAGAAGGTATT
GAGGCGGCTCGGGGTGCATTGCACCATGTCGCGCCGGTCGGCAAGATGGAAGACTACATTGAGCGGATGTTGCGTAATGTCACGTCCCACCTCTGAACGA
GGTGCACTGACCATGCATATCCAACCTACTCCCTTGAGGTCACAACAAAGCTAATGATCGATCTACTTGTTTTCCTCCCGGCATGTTTCGCACTGAACCT
GGCGTTCGGCCCCAATAACCTCGTTGCGATGACACACGGTGCGCAAAAGGGCGTAGGGTTTTCCTTTATGGCAAGCACAGCACGGCTTGCCGTCTTCGTG
CCCATGATTGCTGCTAGCGCGCTTGGTTTAGGGATGCTTTTGTCTGCTTCGGCGTTGGTCTTCAACGTGATCAAGATCGTTGGTGCCGCCTATCTGGTCT
GGATGGGCATCAAGCTCCTCAGAACAACATCTGGCTCGACTAACCTAAACGCAGATGCAATGAAACCTTCGCTTCGTAAGGCATTCCGTAGTGAGGCGCT
GATCGCCATCAGTAACCCCAAAGCAATCCTCATTTTCGCGGCATTCTTCCCGCAATTCGTGGATATAACGAATTACTGGCTGAGCTATGCAACTCTTGGG
ACGGTTTTTATCGCGCTAGAGGCCGTTGCAATAGCGATATATGCGTCAGCAGGGCGTTTTGCAGCAGCTTTCGCAGCAAACAAGCTGCACTGGTTTCAAC
GCGCCTCAGGTGGAGGAATGATTGCTTTCGGCATCCTGCTTCTTCTTAGTCGCCAACCTTCGCGTGTTGCCTAGGTTCTTAAGAACGCTTTAGCCAGTTG
CTAGTCGTCTTAAGAGAATAACGTATGACCGAAATGCCCGACAACATCCTGCACCTGCCCTCATACCAGGTGCTGGGCTGCAAAGCCACCGACGATGAAA
TGCACTTCCAGGTAGACGCGCCCGATCCCATTGCTTGCGAGGAATGCGGGGTGCAGGGTGAGTTCGTGCGTTTCGGCAAGCGTGATGTTCCCTATCATGA
TCTGCCCATCCACGGCAAGCGGGTCACCCTCTGGGTGCTCCGCCGCCGGTACATCTGTCGGGCCTGCGAGGCTACCTTCAGGCCCCAGCTACCGGAGGTG
GTGGACGGCTTCCGCATGACACTGCGGCTACATGAATACGTAGAGAAGGAGTCCTTTAACCACCCCTACACTTTCGTGGCGCAGCAAACCGGATTGGACG
AGAAGACGGTACGGGACATCTTCAACGCCCGCGCCGCGTTCTTAGGCCGCTGGCACCGCTTCGAAACGCCTCGCATTCTGGGCATCGACGAGCTGTACCT
GAACAAGCGCTACCGCTGCATCCTGACCAACATTGAGGAGAAGACCCTGCTCGATCTGCTGGCCACTCGCCGTCAGGACGTCGTGACTAACTACCTGATG
AAGCTGAAAGACCGGCAGAAGATCGATATCGTCAGCATGGACATGTGGAATCCATATCGAACGGCGGTCAGGGCGGTGCTGCCACAGGCCCGCATCGTGG
TCGATAAATTCCATGTGGTGCGCATGGCCAACGATGCTCTGGAGAAGGTGCGCAAGGGGCTCCGGAAAGAGCTGAAACCAGCCCAGATCCGAACCCTCAA
GGGAGACCGGAAAATCCTGCTGAAGCGCGCTCACGAAGTCTCAGACCGGGAGCGGCTGATCATGGAAACCTGGACAGGCGCATTCCCGCAACTGCTGGCT
GCCTACGAGCACAAGGAGCGCTTCTACAGCATCTGGGACGCCACCACACGACCCCAGGCAGAAGCCGCCCTGGACGAGTGGATATCCACCATCCCGAAGG
GCCAGAGAGAAGTCTGGAGCGATCTGTTTAGGGCGGTGGGCAACTGGCGCGAAGAGATCATGACCTACTTCGAGACGGACATACCTGTCACCAACGCTTT
CACGGAGTCCATCAACCGACTGGCCAAGGACAAGAACCGTGAAGGACGAGGTTATTCCTTTGAGGTGATGCGGGCGCGAATGCTCTACACCACGAAGCAC
AAGAAGAAGGCCCCGACTGCGAAGGTCTCTCCGTTCTACAAGAAAACCATCGGTTACGGACTGCCAGACCTCGCAGAGGAGCTCAACTACGGGGTCGATC
TATCAACCATCTGAGGGTAGCATCAGGTTGGTGAGGGGAAGGTGCCCCATCAACCATTAAATCCGCATACCC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
621 bp206 aa4541074+No
ORF function : Passenger Gene
Annotation : LysE-like proteinDescription :

ORF sequence :

MIDLLVFLPACFALNLAFGPNNLVAMTHGAQKGVGFSFMASTARLAVFVPMIAASALGLGMLLSASALVFNVIKIVGAAYLVWMGIKLLRTTSGSTNLNA
DAMKPSLRKAFRSEALIAISNPKAILIFAAFFPQFVDITNYWLSYATLGTVFIALEAVAIAIYASAGRFAAAFAANKLHWFQRASGGGMIAFGILLLLSR
QPSRVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1290 bp429 aa11252414+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MTEMPDNILHLPSYQVLGCKATDDEMHFQVDAPDPIACEECGVQGEFVRFGKRDVPYHDLPIHGKRVTLWVLRRRYICRACEATFRPQLPEVVDGFRMTL
RLHEYVEKESFNHPYTFVAQQTGLDEKTVRDIFNARAAFLGRWHRFETPRILGIDELYLNKRYRCILTNIEEKTLLDLLATRRQDVVTNYLMKLKDRQKI
DIVSMDMWNPYRTAVRAVLPQARIVVDKFHVVRMANDALEKVRKGLRKELKPAQIRTLKGDRKILLKRAHEVSDRERLIMETWTGAFPQLLAAYEHKERF
YSIWDATTRPQAEAALDEWISTIPKGQREVWSDLFRAVGNWREEIMTYFETDIPVTNAFTESINRLAKDKNREGRGYSFEVMRARMLYTTKHKKKAPTAK
VSPFYKKTIGYGLPDLAEELNYGVDLSTI

 

Blast result :
Comments
ISPst9 transposase is 96% aa similar to ISPpu12 transposase (ORF4) and IS1396 and 92% to ISPst2.
Another possible ORF was found and the product showed a certain degree of similarity to bacterial transporter LysE.
References
1] Bosch,R. and Lalucat,J. (2003) Direct submission GenBank