ISClte1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004557 | ND | Clostridium tetani | Clostridium tetani E88 |
DNA section
IS Length : 1466 bp
Ends
IR Length : 32/43
IRL : TGTACCCTATAGAATAGACACTCTAAAAAAAGTCTATCCCGTAGGGTACT
IRR : TGTACCCTTTGTCAAGGACAGTTTAAAAAAAGACTAGGCGGTACTAAGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTAACTAAAA | TTTTAAACTT | 0 |
DNA sequence
TGTACCCTATAGAATAGACACTCTAAAAAAAGTCTATCCCGTAGGGTACTTTTGTGTATAATTAAATAAAGAATAATTGATATTAGGAGAACTGTAATGA
GTAAAAAAATATTTACTGAAAATGAAATAGCGATACTATCTGAAAATAAATTTGTAAAAAAGGTTAGTTGTAAAGGAATTACATATACTGATGAATTTAA
AAGATTATTTATTATGGAAAATAAAAATGGAAAATTTCCACGAAAAATATTCGAAGAATGTGGATTTAATATAGATATTTTAGGTTTAAAGCGTGTTCAA
TCATGTGGAAAAAGATGGCGTGCAGCATTTAGAAAAAGTGGAGTAAATCAACTCCAAGATACTAGGAAATTCAATACTGGAAGACCTACCGAAAAAAATT
TGCCTATTGAAGAAAAGTATGAAAAACTTCAGACTAAAATAAAACTCTTAGAAGCAGAAAATGAACTGCTAAAAAAGTTAGAAAGGTTGGAAAGGAGCGT
GAAAAAGAAAAAGTGAAATTGCTAACGCAACAAAAATATATCCTTATAAAATCAGTTATAGATAAATATAAACTTAAAAATTTAGTTAGTTACTTATGTA
AACTATCTAATGTTTCACGTTCAGGATATTATAATTACTTTTCTATAAAATCTCATACTAATCGTATATCACGCGAAGAGCGTGATTTAGAAAGCTATAA
TAATATATTAATTGCATACAACTTCAAAAAGCGTAAAAAAGGTGCTAGACAAATTAAAATGACTTTAGAAAATCAGTTTCATATTAATTATAACCTAAAG
CGTATTAGAAGAATAATGCATAAATATGATATTATATGTCCGCATAGAAAAGCTAACCCTTATAGAAGAATGATGAAGGCTAACAAAGAACATTCTGTCT
TGCCAAATTTATTAAATAGAGAATTTAAACAAGATGTACCTGGAAAGGTATTGCTCACTGACATAACATATCTATTTTATAAAAATGGTCAAAGGGCTTA
TTTGTCAACCGTTTTAGATGGTTCAACCAATGAATTATTAACCTATAATCTATCTAAAAGTCTAAAGATAGATATTGTGACTGAAACTATTGAAAAATTG
GTTTCATCAAATAACTATTTAATATCGTCAGATTCATTTATTCATTCAGATCAAGGGGCACATTACACTAGTCCCATATTTCAGAAATTGCTTAAAAAAT
ATAATATAAAACAATCTATGTCTAGACGAGGTAACTGCTGGGATAATGCTCCACAGGAGTCATTCTTTGGACATCTTAAGGATGAAACAAATATTAAAAA
TTGTAATACATTTGCTGAACTCTTGGAAGAAATTGATGAATATATGGATTATTATAATAATTATAGAGGTCAATGGAATTTAAAAAAGATGACTCCTGTA
AAATACAGAAATCATCTTCTTAGTACCGCCTAGTCTTTTTTTAAACTGTCCTTGACAAAGGGTACA
GTAAAAAAATATTTACTGAAAATGAAATAGCGATACTATCTGAAAATAAATTTGTAAAAAAGGTTAGTTGTAAAGGAATTACATATACTGATGAATTTAA
AAGATTATTTATTATGGAAAATAAAAATGGAAAATTTCCACGAAAAATATTCGAAGAATGTGGATTTAATATAGATATTTTAGGTTTAAAGCGTGTTCAA
TCATGTGGAAAAAGATGGCGTGCAGCATTTAGAAAAAGTGGAGTAAATCAACTCCAAGATACTAGGAAATTCAATACTGGAAGACCTACCGAAAAAAATT
TGCCTATTGAAGAAAAGTATGAAAAACTTCAGACTAAAATAAAACTCTTAGAAGCAGAAAATGAACTGCTAAAAAAGTTAGAAAGGTTGGAAAGGAGCGT
GAAAAAGAAAAAGTGAAATTGCTAACGCAACAAAAATATATCCTTATAAAATCAGTTATAGATAAATATAAACTTAAAAATTTAGTTAGTTACTTATGTA
AACTATCTAATGTTTCACGTTCAGGATATTATAATTACTTTTCTATAAAATCTCATACTAATCGTATATCACGCGAAGAGCGTGATTTAGAAAGCTATAA
TAATATATTAATTGCATACAACTTCAAAAAGCGTAAAAAAGGTGCTAGACAAATTAAAATGACTTTAGAAAATCAGTTTCATATTAATTATAACCTAAAG
CGTATTAGAAGAATAATGCATAAATATGATATTATATGTCCGCATAGAAAAGCTAACCCTTATAGAAGAATGATGAAGGCTAACAAAGAACATTCTGTCT
TGCCAAATTTATTAAATAGAGAATTTAAACAAGATGTACCTGGAAAGGTATTGCTCACTGACATAACATATCTATTTTATAAAAATGGTCAAAGGGCTTA
TTTGTCAACCGTTTTAGATGGTTCAACCAATGAATTATTAACCTATAATCTATCTAAAAGTCTAAAGATAGATATTGTGACTGAAACTATTGAAAAATTG
GTTTCATCAAATAACTATTTAATATCGTCAGATTCATTTATTCATTCAGATCAAGGGGCACATTACACTAGTCCCATATTTCAGAAATTGCTTAAAAAAT
ATAATATAAAACAATCTATGTCTAGACGAGGTAACTGCTGGGATAATGCTCCACAGGAGTCATTCTTTGGACATCTTAAGGATGAAACAAATATTAAAAA
TTGTAATACATTTGCTGAACTCTTGGAAGAAATTGATGAATATATGGATTATTATAATAATTATAGAGGTCAATGGAATTTAAAAAAGATGACTCCTGTA
AAATACAGAAATCATCTTCTTAGTACCGCCTAGTCTTTTTTTAAACTGTCCTTGACAAAGGGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 420 bp | 139 aa | 97 | 516 | + | No |
Description : First part of the transposase
ORF sequence :
MSKKIFTENEIAILSENKFVKKVSCKGITYTDEFKRLFIMENKNGKFPRKIFEECGFNIDILGLKRVQSCGKRWRAAFRKSGVNQLQDTRKFNTGRPTEK
NLPIEEKYEKLQTKIKLLEAENELLKKLERLERSVKKKK
NLPIEEKYEKLQTKIKLLEAENELLKKLERLERSVKKKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 969 bp | 322 aa | 465 | 1433 | + | No |
Description : Second part of the transposase
ORF sequence :
TAKKVRKVGKEREKEKVKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLESYNNILIAYNFKKRKKGARQIKMT
LENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRMMKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQRAYLSTVLDGSTNELLTYNLSKSL
KIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRGNCWDNAPQESFFGHLKDETNIKNCNTFAELLEEIDEYMDYYNNY
RGQWNLKKMTPVKYRNHLLSTA
LENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRMMKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQRAYLSTVLDGSTNELLTYNLSKSL
KIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRGNCWDNAPQESFFGHLKDETNIKNCNTFAELLEEIDEYMDYYNNY
RGQWNLKKMTPVKYRNHLLSTA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1337 bp | 445 aa | 97 | 1433 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKKIFTENEIAILSENKFVKKVSCKGITYTDEFKRLFIMENKNGKFPRKIFEECGFNIDILGLKRVQSCGKRWRAAFRKSGVNQLQDTRKFNTGRPTEK
NLPIEEKYEKLQTKIKLLEAENELLKKVRKVGKEREKEKVKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLES
YNNILIAYNFKKRKKGARQIKMTLENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRMMKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQR
AYLSTVLDGSTNELLTYNLSKSLKIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRGNCWDNAPQESFFGHLKDETNI
KNCNTFAELLEEIDEYMDYYNNYRGQWNLKKMTPVKYRNHLLSTA
NLPIEEKYEKLQTKIKLLEAENELLKKVRKVGKEREKEKVKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLES
YNNILIAYNFKKRKKGARQIKMTLENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRMMKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQR
AYLSTVLDGSTNELLTYNLSKSLKIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRGNCWDNAPQESFFGHLKDETNI
KNCNTFAELLEEIDEYMDYYNNYRGQWNLKKMTPVKYRNHLLSTA
Blast result :
Comments
ISClte1 is 54% aa similar to ISFnu6 (ORF1) and 51% to IS1223 (ORF2).
There are 9 full copies in Clostridium tetani E88 genome.
There are 9 full copies in Clostridium tetani E88 genome.
References
1] Bruggemann,H., Baumer,S., Fricke,W.F., Wiezer,A., Liesegang,H., Decker,I., Herzberg,C., Martinez-Arias,R., Merkl,R., Henne,A. and Gottschalk,G. (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (3), 1316-1321