ISSau5

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_002952 ND Staphylococcus aureus
Staphylococcus aureus subsp. aureus MRSA252
DNA section
IS Length : 1136 bp

Ends


IR Length : 7/12

IRL : CAACTCCCTATACGCTTTAGCGGTGGTATTGGGAGTTGATGAACATATAT
IRR : GATGAACCTATATTAATTGACGGGTTCGTGCCTCACACAGTCATTTAATA

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

CAACTCCCTATACGCTTTAGCGGTGGTATTGGGAGTTGATGAACATATATCCTCTTATAACTTATTTTATAAATAACCTTTTTAAAAGTAAAGGAGGATA
TACATGAAAAAAAGAAAAAAACGTAAAGAATTAGACTATAAAACACGTCTAGCTATCGCAAAGTATTTAAATGATGGTTTAAACATCATTCAGATTTCTA
AGATTTTAGGTCGTGATGATGCGGTTCTTTATAGAGAAGTAAATAAACGTTCTGTTGATGGTGTTTATAATCCTTTATTAGCTGAGGAAGATGCTATAAA
ATCGCGTAGTATTTCAGATGAACAAGTTGCTAAACGTAGACGTTTGAATGATGAAACAAAAGCATATATTGAAGAAAAACTTTCATTAAAATGGTCTCCT
AGACAGATAGCAAGTCAAATTGAAAAAGATACAGGGCATTATATAAGTTATCCTACTATATATAGATATATTCGCAATGGATTGGTAAAAGTTGATGTTA
AGCGTGATATGAGACAAGCAGGCAAGAAATATAATAAGTCTTCTGAAAAGCGTGGCAAATTAGACGTAGGGTCTCGTGTTATAAAGTATCGGCTCAAAAA
GTATTAATAATCGTGAAGAATTTGGACATTATGAAATTGATACTGTTTGGAGTGTTAGACCATCAACATATTGCTTGTTAACTATTATTGAAAGAAAAAC
AAGATATTTATATGCTAGTAGATTAAATACACGTAAATCCAATGTAGTTTGTAATGAGATTATAAATATTATGAAACCATTATTACCCAAAAGTATAACT
ATGGATAGAGGTAAGGAGTTTGCTCTATTTAAACAAATAGAAGATGAATTGAATTGTAGTATTTATTTTAGTGATTTGGGTTGTCCATATCAACGTGGTT
CCATTGAAAATGCAAATGGACTTTTAAGACAGTATTATCCTAAAGGTACGGATTTTGCAAATATAACACAAAAGTCATTAGATGAAGCTGTTAAACAAAT
TAATGCACGACCTCGAATGATATTTGACTATAAATCGAGTGAGGAAATGCTAAAGTACCATGTAAGCACACAAAATTGTGAACCTATATTAAATGACTGT
GTGAGGCACGAACCCGTCAATTAATATAGGTTCATC
Recoding section
  • Recoding by frameshift
  • Frame +1
  • Type unknow
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence : No
  • Secondary structure : No-structure

Recoding motif :

AGTATCGGCTC AAAAAG TATTAATAATCGT
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
492 bp163 aa104595+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYREVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPR
QIASQIEKDTGHYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVIKYRLKKY

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
528 bp175 aa5971124+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SIGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYASRLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFKQIEDELNCSIYFSDLGC
PYQRGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVSTQNCEPILNDCVRHEPVN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1021 bp340 aa1041124+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYREVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPR
QIASQIEKDTGHYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVIKYRLKKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKT
RYLYASRLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFKQIEDELNCSIYFSDLGCPYQRGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQI
NARPRMIFDYKSSEEMLKYHVSTQNCEPILNDCVRHEPVN

 

Blast result :
Comments
ISSau5 is 57% aa similar to ISSau1. The end are not clearely determined. There is a potential frameshift.
There are 1 full copy in Staphylococcus aureus subsp. aureus MRSA252 genome.
References
1] Holden,M.T.G., Feil,E.J., Lindsay,J.A., Peacock,S.J., Day,N.P.J., Enright,M.C., Foster,T.J., Moore,C.E., Hurst,L., Atkin,R., Barron,A., Bason,N., Bentley,S.D., Chillingworth,C., Chillingworth,T., Churcher,C., Clark,L., Corton,C., Cronin,A., Doggett,J., Dowd,L., Feltwell,T., Hance,Z., Harris,B., Hauser,H., Holroyd,S., Jagels,K., James,K.D., Lennard,N., Line,A., Mayes,R., Moule,S., Mungall,K., Ormond,D., Quail,M.A., Rabbinowitsch,E., Rutherford,K., Sanders,M., Sharp,S., Simmonds,M., Stevens,K., Whitehead,S., Barrell,B.G., Spratt,B.G. and Parkhill,J.(2004)Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9786-9791