ISFnu6
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003454 | ND | Fusobacterium nucleatum | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 |
DNA section
IS Length : 1444 bp
Ends
IR Length : 21/27
IRL : TGAACTGCACCCAAAATCTTGGACACAAGATTGGAGGTGCAGTTTTTTTG
IRR : TGTACTGACCCCAAAAAGTTGGACAAATTTAATTTAACTTACTAATAAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATAAATAGAG | TTT | TCTCTAAATA | 3 |
DNA sequence
TGAACTGCACCCAAAATCTTGGACACAAGATTGGAGGTGCAGTTTTTTTGAGTAAATTAACAAGAGAAGAGAAAATTGAAATATTTGAAAGAAGAAAAAT
GGGTGAAACTATTTCTTCTTTAGCAAAAGCTTTTAATATTCATGAATCTAATATTAAATATTTAATTGCTTTAATTGAAAAATATGGAAATAATATTTTA
AGAAAAGGTAAGAATAGAGCTTATTCAAAAGAATTTAAGTTACAGGCAATTAATAGAATTTTAATTAATCATGAGTCTATAAATTCTGTTGCTCTTGATA
TTGGTTTAGTATCTGCTAGTATTTTACATAATTGGCTTTCAAAATTTAAGGAAAACGAGTATAATGTTGTAGAGAAGAAAAAAGGAAGGAAACCCAAATC
TATGACTAAACCTAAGAAAAATGACAAAGAATTATCTGAAAAAGAAAAAATTAAAAAATTAGAAGAAGAAAATTTGTATCTTAAAGCTGAGAATGAATAC
TTAAAAAAATTGAGGGCTCTAGTTCAAGAAAGGGAGCTAAAAGAGAAGAAAAAGTAAGAGTAATAGCAGAACTTAGAGCTAAATACCCCTTCAAAATATT
ATTAAAGATTGCTGGAATATCAAGATCAGTATATTATTACTATATTGATAAAAAAGATATTGATGAGAAGAATAAAGGTGTTATTGAAAAAATCAAAGAA
ATTTACTATGCGAATAAAGGAAGATATGGTTATCGCAGAGTAACATTAGAATTAAAAAATCAAGGTTTCAATATTAATCATAAAAAAGTACAGAGACTTA
TGAAGAAATTTGATTTACAAAGTATTATCCGTAAAAAAAGAAAATATTCTTCATATAAAGGTCAAGTGGGTAAAATAGCTGATAATCATATTAAGAGAGA
TTTTGAAGCAACAGCTCCAAATCAAAAATGGTTTACAGATGTAACAGAATTTAATTTAAGAGGAGAAAAGCTATACTTATCTCCAATATTAGATGCTTAT
GGAAGATATATAGTTTCGTATGATATTTCGCGCAGTCCTAACTTGGAGCAGATAAATCATATGTTAAATTTAGCATTTAAAGAAAAGGAAAACTATGAAA
ATTTGATATTTCATAGTGATCAAGGATGGCAGTATCAGCATTATTCATATCAAGAAAGATTAAAAGAGAAGAAGATAACTCAAAGTATGTCAAGAAAAGG
AAATAGTTTAGATAATGGATTAATGGAATGCTTCTTTGGATTATTAAAATCAGAAATGTTTTATGAGCAAGAAGAAAAATACAAAACATTGGAAGAATTG
AAAGAAGCAATAGAAGATTATATATATTATTACAATAACAAAAGAATAAAGGAAAAATTAAAAGGATTAACTCCTGCTTCTTACAGAAGTCAATCCTTAT
TAGTAAGTTAAATTAAATTTGTCCAACTTTTTGGGGTCAGTACA
GGGTGAAACTATTTCTTCTTTAGCAAAAGCTTTTAATATTCATGAATCTAATATTAAATATTTAATTGCTTTAATTGAAAAATATGGAAATAATATTTTA
AGAAAAGGTAAGAATAGAGCTTATTCAAAAGAATTTAAGTTACAGGCAATTAATAGAATTTTAATTAATCATGAGTCTATAAATTCTGTTGCTCTTGATA
TTGGTTTAGTATCTGCTAGTATTTTACATAATTGGCTTTCAAAATTTAAGGAAAACGAGTATAATGTTGTAGAGAAGAAAAAAGGAAGGAAACCCAAATC
TATGACTAAACCTAAGAAAAATGACAAAGAATTATCTGAAAAAGAAAAAATTAAAAAATTAGAAGAAGAAAATTTGTATCTTAAAGCTGAGAATGAATAC
TTAAAAAAATTGAGGGCTCTAGTTCAAGAAAGGGAGCTAAAAGAGAAGAAAAAGTAAGAGTAATAGCAGAACTTAGAGCTAAATACCCCTTCAAAATATT
ATTAAAGATTGCTGGAATATCAAGATCAGTATATTATTACTATATTGATAAAAAAGATATTGATGAGAAGAATAAAGGTGTTATTGAAAAAATCAAAGAA
ATTTACTATGCGAATAAAGGAAGATATGGTTATCGCAGAGTAACATTAGAATTAAAAAATCAAGGTTTCAATATTAATCATAAAAAAGTACAGAGACTTA
TGAAGAAATTTGATTTACAAAGTATTATCCGTAAAAAAAGAAAATATTCTTCATATAAAGGTCAAGTGGGTAAAATAGCTGATAATCATATTAAGAGAGA
TTTTGAAGCAACAGCTCCAAATCAAAAATGGTTTACAGATGTAACAGAATTTAATTTAAGAGGAGAAAAGCTATACTTATCTCCAATATTAGATGCTTAT
GGAAGATATATAGTTTCGTATGATATTTCGCGCAGTCCTAACTTGGAGCAGATAAATCATATGTTAAATTTAGCATTTAAAGAAAAGGAAAACTATGAAA
ATTTGATATTTCATAGTGATCAAGGATGGCAGTATCAGCATTATTCATATCAAGAAAGATTAAAAGAGAAGAAGATAACTCAAAGTATGTCAAGAAAAGG
AAATAGTTTAGATAATGGATTAATGGAATGCTTCTTTGGATTATTAAAATCAGAAATGTTTTATGAGCAAGAAGAAAAATACAAAACATTGGAAGAATTG
AAAGAAGCAATAGAAGATTATATATATTATTACAATAACAAAAGAATAAAGGAAAAATTAAAAGGATTAACTCCTGCTTCTTACAGAAGTCAATCCTTAT
TAGTAAGTTAAATTAAATTTGTCCAACTTTTTGGGGTCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 459 bp | 152 aa | 99 | 557 | + | No |
Description : First part of the transposase
ORF sequence :
MGETISSLAKAFNIHESNIKYLIALIEKYGNNILRKGKNRAYSKEFKLQAINRILINHESINSVALDIGLVSASILHNWLSKFKENEYNVVEKKKGRKPK
SMTKPKKNDKELSEKEKIKKLEEENLYLKAENEYLKKLRALVQERELKEKKK
SMTKPKKNDKELSEKEKIKKLEEENLYLKAENEYLKKLRALVQERELKEKKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 497 | 1411 | + | No |
Description : Second part of the transposase
ORF sequence :
ILKKIEGSSSRKGAKREEKVRVIAELRAKYPFKILLKIAGISRSVYYYYIDKKDIDEKNKGVIEKIKEIYYANKGRYGYRRVTLELKNQGFNINHKKVQR
LMKKFDLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVTEFNLRGEKLYLSPILDAYGRYIVSYDISRSPNLEQINHMLNLAFKEKENY
ENLIFHSDQGWQYQHYSYQERLKEKKITQSMSRKGNSLDNGLMECFFGLLKSEMFYEQEEKYKTLEELKEAIEDYIYYYNNKRIKEKLKGLTPASYRSQS
LLVS
LMKKFDLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVTEFNLRGEKLYLSPILDAYGRYIVSYDISRSPNLEQINHMLNLAFKEKENY
ENLIFHSDQGWQYQHYSYQERLKEKKITQSMSRKGNSLDNGLMECFFGLLKSEMFYEQEEKYKTLEELKEAIEDYIYYYNNKRIKEKLKGLTPASYRSQS
LLVS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1313 bp | 437 aa | 99 | 1411 | + | Yes |
Chemistry : DDE
ORF sequence :
MGETISSLAKAFNIHESNIKYLIALIEKYGNNILRKGKNRAYSKEFKLQAINRILINHESINSVALDIGLVSASILHNWLSKFKENEYNVVEKKKGRKPK
SMTKPKKNDKELSEKEKIKKLEEENLYLKAENEYLKKIEGSSSRKGAKREEKVRVIAELRAKYPFKILLKIAGISRSVYYYYIDKKDIDEKNKGVIEKIK
EIYYANKGRYGYRRVTLELKNQGFNINHKKVQRLMKKFDLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVTEFNLRGEKLYLSPILDA
YGRYIVSYDISRSPNLEQINHMLNLAFKEKENYENLIFHSDQGWQYQHYSYQERLKEKKITQSMSRKGNSLDNGLMECFFGLLKSEMFYEQEEKYKTLEE
LKEAIEDYIYYYNNKRIKEKLKGLTPASYRSQSLLVS
SMTKPKKNDKELSEKEKIKKLEEENLYLKAENEYLKKIEGSSSRKGAKREEKVRVIAELRAKYPFKILLKIAGISRSVYYYYIDKKDIDEKNKGVIEKIK
EIYYANKGRYGYRRVTLELKNQGFNINHKKVQRLMKKFDLQSIIRKKRKYSSYKGQVGKIADNHIKRDFEATAPNQKWFTDVTEFNLRGEKLYLSPILDA
YGRYIVSYDISRSPNLEQINHMLNLAFKEKENYENLIFHSDQGWQYQHYSYQERLKEKKITQSMSRKGNSLDNGLMECFFGLLKSEMFYEQEEKYKTLEE
LKEAIEDYIYYYNNKRIKEKLKGLTPASYRSQSLLVS
Blast result :
Comments
ISFnu6 is 67%(ORF A) and 74% (ORF B)aa similar to IS861 .
There are 4 full and 16 partial copies of ISFnu6 in Fusobacterium nucleatum subsp. nucleatum genome.
There are 4 full and 16 partial copies of ISFnu6 in Fusobacterium nucleatum subsp. nucleatum genome.
References
1] Kapatral,V., Anderson,I., Ivanova,N., Reznik,G., Los,T., Lykidis,A., Bhattacharyya,A., Bartman,A., Gardner,W., Grechkin,G., Zhu,L., Vasieva,O., Chu,L., Kogan,Y., Chaga,O., Goltsman,E., Bernal,A., Larsen,N., D'Souza,M., Walunas,T., Pusch,G.,Haselkorn,R., Fonstein,M., Kyrpides,N. and Overbeek,R. (2002) J. Bacteriol. 184 (7), 2005-2018