ISBmu3

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB159775 Y Burkholderia multivorans
Burkholderia multivorans ATCC17616
DNA section
IS Length : 2697 bp

Ends


IR Length : 40/49

IRL : TGCGTATTTCGGCGAGCGTGATCAGCGATTTCGGTTGAACGTGATCAGTG
IRR : TGCGGATTTCGGTGATGGTGATCAGGCGTTTCGGCGAACGTGATCAGTTT

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNTTTGCGAANNNNNNNNNN8
NNNNNNNNNNGATTGCANNNNNNNNNN7

DNA sequence

TGCGTATTTCGGCGAGCGTGATCAGCGATTTCGGTTGAACGTGATCAGTGGTTCCGATTGATGGTGATCGGTCGTTTCGGTCGAAGGTGATCACTTATCA
CCGGTTTCCGAAACGAGCGATCATCTTGCCGAAGCGGGTGATCACGTTCCCGGAATGAGTGATCAGCGAACCGAAACAGCTGATCACGCGACCGAAACGG
GATAGCCGTTCCGGCTCGGCGGTGGTGTTGCGCATGTGATCAACGACGGGCGTTAGCCTTGATCCTTTTTAGGGGACAGGTTGATGCCGGCACATCGGAT
GAGCATGCGCAAACTGAAGGAAGTACTGCGGCTGAAGTGGGCGTGCGGCCTGTCGCACCGCCAGATCAGCCGCGCGATCGGCATCAGCGTCGGCGCCATC
TCGGCATACGCCGCCCGCGCGAGCGCGGCGGGCCTCGACTGGGCTACCGTCGAACCGCTCGCCGACGACGAGCTCGAGATCAGGTTGGACCTGCCGGAGG
AAACCGCGGTCCCGACCCGCCGGGTCGAACCGGATTACGCGGCGATGCACCGCGACCTGCGCCGCAAGGGCGTGACGCTGCAACTGCTCTGGGAGGAGTA
CGTCGAGGCTCACCCAGGCCAACGCACCTATCGCTATACGCAGTTCTGCCAGCGGTACAAGGACTGGGCTGCGGCGCTGAAGCGCTCGATGCGCCAGCAA
CACCGCGCCGGCGAGAAGCTGTTCGCCGACTTCGCCGGGCAAACTGTGCCGATCCTCGGTCGCGACGGCGGCATCGCGTTCAAGGCTCACGTGTTCGTGG
CCGTCCTCGGCGCCTCGAACTACACCTATGCATGTGCGACGCGTTCGGAGGCCATGCCCGACTGGATCGGCAGCCTGATCGACGCGTTGGAATTCTACGG
CGGTGTCCCCGAGCTGCTGGTGCCCGACAACCCCAAGGCATTGATCGCCAAGGCGGACCGGTACGAACCGGTGCTGGGCAACACCACGCAGGACTTCGTG
AACCACTACGCGACCGCCATGTTGCCGGCGCGGCCGCGCAAGCCGCAGGACAAGGCGAAGGTCGAGGTCGGCGTGCAGATCGTCGAGCGCTGGATCCTCG
CACGGCTGCGCAACCACCGCTTCTACAGCCTGGCCGAGCTGAACAAGGCGATCGGCAAGCTGATCACCGACCTGAACCGGCGTCCGTTCAAGAAGCTCGA
CGGCAACCGCCGCGAATGGTTCGAGCGGCTAGACCGCCCGGCGCTGCGCCCGTTGCCGACACGCCGATACGAGATCGCGACCTTCGTGAAGTGCCGCGTC
AACATCGACTACCACGTCGAAGTCGATCACCACTATTACAGCGTCCCGCACAGCCTCGTGCGCCAGGAAGTCTATGCCCGCGTGACGCGCCACGGCGTCG
AGATCTTGCACCGCGGCAAGCGCGTTGCCGCCCACGCCCGCAGCCGACTCCGGAACAAGCATACGACGTTGCCTGAGCATATGCCGGCGGCGCACCGCGC
CCACATGGAATGGACGCCAGGCCGGTTGTTGAACTGGGGCGCCTCGGTCGGTCCCGGTGCCGAGGCGATCGTCAAACATCTGTTGACCAACCGGCCCCAT
CCCGAGATGGGGTACCGTGCCTGTCTCGGACTGCTGTCGCTCTCGCGCAAGTACGGCAAGGAACGGCTGGAAGCCGCCTGCCAGCGGGCGCTCGTGATCG
GCTCACCGACCCGCCGCTCCGTGCTGTCGATCCTCGAATCCGGCCTGGACCGACAGCCGATGCTCCCGATTCCGCCCGCCGAATGGCATTCGCCCGATCA
CGAGAACGTGCGCGGGCCCGAGTATTACCACTGACCCAAGGAGGTCGCAATGTTGATGCAGCAAACCTTAACCCAGCTCAAGTCCCTGAAGCTCGACGGC
ATGGCCCGCGCGTTCGAGGAACAAACGGCGTTGACCGCCAGCACCAGCCTGTCGTTCGAAGAGCGCTTCGGCATGGTCGTCGAGCGCGAGCTCGCCTGGC
GCGATACCCGGCGGCTCGAACGGCTGCTGAAGTCTGCGAAACTCAAGAATCCCCAGGCCTGCATTGAGGACATCGAATACCGGCAGAGCCGCGGCATCGA
CAAGAGCGTCGTCGCCGCACTTGCCGGTTGCGACTGGATCCGTAACGCGCAGAACCTCATCCTGACGGGGCCGACCGGCGCAGGCAAGACGTGGATTGCG
TGCGCGTTCGGTCAGCAGGCCTGCCGACAAGGCTTCTCCGTGATGTACGTCCGCGTTGCCCGGCTGTTCGAGGAATTGAAGATCGCTCACGGCGACGGCA
GCTTCACGCGGCGGCTCGCGCAGCTCGCCAAGATGGACGTCCTGCTGCTCGACGACTGGGGGCTGCAGGATCTCGATCAGGGTGCCCGAAACGATCTGCT
CGAAGTGCTCGACGATCGCGTTGGCACGCGCTCCACGATCATTACCAGCCAACTGCCATTGGAACACTGGCACGCTTGGCTTCAGGACCCGACGCTCGCC
GACGCGATCCTCGACCGGCTCGTCCATCAGGCCCACAAGCTGCCGTTGAAGGGCGAGTCGATGCGAAAGACCAACGCCAAGCAGTCCTCCGCATCCTGAT
CGTGATCACCTTCGCTATAATCCATTGACCGCGCAACACCACCTTCCAAACTGATCACGTTCGCCGAAACGCCTGATCACCATCACCGAAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1551 bp516 aa2841834+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPAHRMSMRKLKEVLRLKWACGLSHRQISRAIGISVGAISAYAARASAAGLDWATVEPLADDELEIRLDLPEETAVPTRRVEPDYAAMHRDLRRKGVTLQ
LLWEEYVEAHPGQRTYRYTQFCQRYKDWAAALKRSMRQQHRAGEKLFADFAGQTVPILGRDGGIAFKAHVFVAVLGASNYTYACATRSEAMPDWIGSLID
ALEFYGGVPELLVPDNPKALIAKADRYEPVLGNTTQDFVNHYATAMLPARPRKPQDKAKVEVGVQIVERWILARLRNHRFYSLAELNKAIGKLITDLNRR
PFKKLDGNRREWFERLDRPALRPLPTRRYEIATFVKCRVNIDYHVEVDHHYYSVPHSLVRQEVYARVTRHGVEILHRGKRVAAHARSRLRNKHTTLPEHM
PAAHRAHMEWTPGRLLNWGASVGPGAEAIVKHLLTNRPHPEMGYRACLGLLSLSRKYGKERLEAACQRALVIGSPTRRSVLSILESGLDRQPMLPIPPAE
WHSPDHENVRGPEYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
750 bp249 aa18502599+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLMQQTLTQLKSLKLDGMARAFEEQTALTASTSLSFEERFGMVVERELAWRDTRRLERLLKSAKLKNPQACIEDIEYRQSRGIDKSVVAALAGCDWIRNA
QNLILTGPTGAGKTWIACAFGQQACRQGFSVMYVRVARLFEELKIAHGDGSFTRRLAQLAKMDVLLLDDWGLQDLDQGARNDLLEVLDDRVGTRSTIITS
QLPLEHWHAWLQDPTLADAILDRLVHQAHKLPLKGESMRKTNAKQSSAS

 

Blast result :
Comments
ISBmu3 is 90% (orf1)and 96% (ORF2) aa similar to IS408.
References
1] Yoshiyuki Ohtsubo, Hiroyuki Genka, Harunobu Komatsu, Yuji Nagata, and Masataka Tsuda,
Applied and Environmental Microbiology, in preparation.
High-Temperature-Induced Transposition of IS Elements in Burkholderia multivorans ATCC 17616