ISTth1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005838 | ND | Thermus thermophilus | Thermus thermophilus HB27 plasmid pTT27 |
DNA section
IS Length : 1261 bp
Ends
IR Length : 24/28
IRL : TGAAGTGCTGCCCCAAATTTGGACCACCCCAAGAGAGAGGCCGAGGGGTG
IRR : TGTACTGCGGCCCTAAATTTGGACCACCTTACCCGTCAGTTTAGTCAGGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGAAGTGCTGCCCCAAATTTGGACCACCCCAAGAGAGAGGCCGAGGGGTGTAGTCTGAAGGGGTAGCGAGATGCGCATGTGGTCAGCGAAGGAAAAGGCA
GAAATTGTGTTGGAGGTCTTGTCGGGTCAAAGGACCGTAGCCGAAGCCTGCCGGGCTTATGGGGTGGCGGAGTCCCTCTTTTACCGCTGGCAGCGGGAGT
TCCTGGAGAACGCCTACGCCGCCTTCACCTCCGGCTGTGCGGAGCAGGAGGCCCGCATCCGCGAGCTGGAACGCCTGGCGGGCCAGCTGGCCCTGGAGGT
GGAGGTCCTAAAAAAAGCCTCGGGGCTCTACCGGCAAAGGAAAGGCGGGAGCTGGTGAAGGCCCTCAAGGTGTTCCCCTGACGGGGAAGCAACAAGGGAG
CCTACCCCCTCCGCCTGCTTTCCCGGGTCCTCGGGGTGCCCCGGAGCACCCTCTACTACCGGCCCCAGGACCCCCCTCCTGAGGAGGCGGCCCTCCGGGG
ACGACTGCGGGCCCTGGCGGAGGCCTGGCCCCGGTACGGCTACCGGCGCCTCACCGCCCTCCTGCGGGGGGAAGGCTTCCAGGTGGGGGAGAAGCGGGTG
CGCTCCCTGATGCGCGAGGAGGGCTTGCTCCTTCCCCGCAAGGGGCCCTCCCCCAGGACCACCTCCCCAGGGGGCCTTCTCCCGGAAGGGGTGAAGAACC
TCCTGCCGGGGCTTGAGGTGACCCGCCCGCACCAGGTGTGGGTGGCGGACCTGAGCTACGTGGTCCTGGGGGAGGGGGTGGCCTACCTGGCGGTGGTGAT
GGACCTCCACACCCGGAAGGTGCTGGGGGTGGCCCTGGGGCCGAGGCTGTCCCAGGGGCTTGCTCTTGCGGCCCTGGAGATGGCCCTGAGGGAGGGGTGT
CCGGAGGTGCACCATTCGGACCGGGGGGTGCAGTACACCTCCAGGGCCTATGTGGAGCGGCTTTTGGGGCTCGGGGTGCGGCTCAGTTACGCGGGGACGG
GGAGGCCTTGGGAGAACGGGCACGCGGAGCGGTTGATCCGGACGATCAAAGAGGAGTGGGTGGCCTTGCGGGAGTACAGGACCCTGGCGGAGGCGCGGGC
TTCGGTGAAGGCTTTTGTCCTTGAGGTGTACGACCGCAAGCGTCCTCACTCGGCCCTGGGCTACCTGACGCCCGAGGCCTTTTCGGAGAGCCTGTGGAAA
GGGGGTGGGAGTCCTGACTAAACTGACGGGTAAGGTGGTCCAAATTTAGGGCCGCAGTACA
GAAATTGTGTTGGAGGTCTTGTCGGGTCAAAGGACCGTAGCCGAAGCCTGCCGGGCTTATGGGGTGGCGGAGTCCCTCTTTTACCGCTGGCAGCGGGAGT
TCCTGGAGAACGCCTACGCCGCCTTCACCTCCGGCTGTGCGGAGCAGGAGGCCCGCATCCGCGAGCTGGAACGCCTGGCGGGCCAGCTGGCCCTGGAGGT
GGAGGTCCTAAAAAAAGCCTCGGGGCTCTACCGGCAAAGGAAAGGCGGGAGCTGGTGAAGGCCCTCAAGGTGTTCCCCTGACGGGGAAGCAACAAGGGAG
CCTACCCCCTCCGCCTGCTTTCCCGGGTCCTCGGGGTGCCCCGGAGCACCCTCTACTACCGGCCCCAGGACCCCCCTCCTGAGGAGGCGGCCCTCCGGGG
ACGACTGCGGGCCCTGGCGGAGGCCTGGCCCCGGTACGGCTACCGGCGCCTCACCGCCCTCCTGCGGGGGGAAGGCTTCCAGGTGGGGGAGAAGCGGGTG
CGCTCCCTGATGCGCGAGGAGGGCTTGCTCCTTCCCCGCAAGGGGCCCTCCCCCAGGACCACCTCCCCAGGGGGCCTTCTCCCGGAAGGGGTGAAGAACC
TCCTGCCGGGGCTTGAGGTGACCCGCCCGCACCAGGTGTGGGTGGCGGACCTGAGCTACGTGGTCCTGGGGGAGGGGGTGGCCTACCTGGCGGTGGTGAT
GGACCTCCACACCCGGAAGGTGCTGGGGGTGGCCCTGGGGCCGAGGCTGTCCCAGGGGCTTGCTCTTGCGGCCCTGGAGATGGCCCTGAGGGAGGGGTGT
CCGGAGGTGCACCATTCGGACCGGGGGGTGCAGTACACCTCCAGGGCCTATGTGGAGCGGCTTTTGGGGCTCGGGGTGCGGCTCAGTTACGCGGGGACGG
GGAGGCCTTGGGAGAACGGGCACGCGGAGCGGTTGATCCGGACGATCAAAGAGGAGTGGGTGGCCTTGCGGGAGTACAGGACCCTGGCGGAGGCGCGGGC
TTCGGTGAAGGCTTTTGTCCTTGAGGTGTACGACCGCAAGCGTCCTCACTCGGCCCTGGGCTACCTGACGCCCGAGGCCTTTTCGGAGAGCCTGTGGAAA
GGGGGTGGGAGTCCTGACTAAACTGACGGGTAAGGTGGTCCAAATTTAGGGCCGCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 288 bp | 95 aa | 71 | 358 | + | No |
Description : First part of the transposase
ORF sequence :
MRMWSAKEKAEIVLEVLSGQRTVAEACRAYGVAESLFYRWQREFLENAYAAFTSGCAEQEARIRELERLAGQLALEVEVLKKASGLYRQRKGGSW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 786 bp | 261 aa | 436 | 1221 | + | No |
Description : Second part of the transposase
ORF sequence :
VPRSTLYYRPQDPPPEEAALRGRLRALAEAWPRYGYRRLTALLRGEGFQVGEKRVRSLMREEGLLLPRKGPSPRTTSPGGLLPEGVKNLLPGLEVTRPHQ
VWVADLSYVVLGEGVAYLAVVMDLHTRKVLGVALGPRLSQGLALAALEMALREGCPEVHHSDRGVQYTSRAYVERLLGLGVRLSYAGTGRPWENGHAERL
IRTIKEEWVALREYRTLAEARASVKAFVLEVYDRKRPHSALGYLTPEAFSESLWKGGGSPD
VWVADLSYVVLGEGVAYLAVVMDLHTRKVLGVALGPRLSQGLALAALEMALREGCPEVHHSDRGVQYTSRAYVERLLGLGVRLSYAGTGRPWENGHAERL
IRTIKEEWVALREYRTLAEARASVKAFVLEVYDRKRPHSALGYLTPEAFSESLWKGGGSPD
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 382 aa | 71 | 1221 | + | Yes |
Chemistry : DDE
ORF sequence :
MRMWSAKEKAEIVLEVLSGQRTVAEACRAYGVAESLFYRWQREFLENAYAAFTSGCAEQEARIRELERLAGQLALEVEVLKKSLGALPAKERRELVKALK
VFPRGSNKGAYPLRLLSRVLGVPRSTLYYRPQDPPPEEAALRGRLRALAEAWPRYGYRRLTALLRGEGFQVGEKRVRSLMREEGLLLPRKGPSPRTTSPG
GLLPEGVKNLLPGLEVTRPHQVWVADLSYVVLGEGVAYLAVVMDLHTRKVLGVALGPRLSQGLALAALEMALREGCPEVHHSDRGVQYTSRAYVERLLGL
GVRLSYAGTGRPWENGHAERLIRTIKEEWVALREYRTLAEARASVKAFVLEVYDRKRPHSALGYLTPEAFSESLWKGGGSPD
VFPRGSNKGAYPLRLLSRVLGVPRSTLYYRPQDPPPEEAALRGRLRALAEAWPRYGYRRLTALLRGEGFQVGEKRVRSLMREEGLLLPRKGPSPRTTSPG
GLLPEGVKNLLPGLEVTRPHQVWVADLSYVVLGEGVAYLAVVMDLHTRKVLGVALGPRLSQGLALAALEMALREGCPEVHHSDRGVQYTSRAYVERLLGL
GVRLSYAGTGRPWENGHAERLIRTIKEEWVALREYRTLAEARASVKAFVLEVYDRKRPHSALGYLTPEAFSESLWKGGGSPD
Blast result :
Comments
ISTth1 is 57% aa similar to ISCc3 (ORF1), 53% to IS120 (ORF2) and 48% to IS1302 (Fusion ORF)
ISTth1 ORF3 is a fusion ORF.
There is one copy in the pTT27 plasmid at coordinates : 183527-184787 and one extremity : IRL 148207 (1)-148253 (47) with one mismatch at position 15 ("G" instead of "A").
ISTth1 ORF3 is a fusion ORF.
There is one copy in the pTT27 plasmid at coordinates : 183527-184787 and one extremity : IRL 148207 (1)-148253 (47) with one mismatch at position 15 ("G" instead of "A").
References
1] Henne,A., Bruggemann,H., Raasch,C., Wiezer,A., Hartsch,T., Liesegang,H., Johann,A., Lienard,T., Gohl,O., Martinez-Arias,R., Jacobi,C., Starkuviene,V., Schlenczeck,S., Dencker,S., Huber,R., Klenk,H.P., Kramer,W., Merkl,R., Gottschalk,G. and Fritz,H.J. (2004) Nat. Biotechnol. 22 (5), 547-553