ISDra3
- Family IS630
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AE000513 | ND | Deinococcus radiodurans | Deinococcus radiodurans Deinococcus radiodurans plasmid pMP1 Deinococcus radiodurans plasmid pCP1 |
DNA section
IS Length : 1065 bp
Ends
IR Length : 20/25
IRL : TTGACTCCGGCACGACTTGGAGACGCGAGGTAAACTTAGGAGGTGCTGCG
IRR : TTGACTCCGGCTTAGGTTGGAGACGAACAGTAGCGGTGAGTAAGGCGCTG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CCTCTCGTAC | TA | GGAGCAACTC | 2 |
DNA sequence
TTGACTCCGGCACGACTTGGAGACGCGAGGTAAACTTAGGAGGTGCTGCGCGTCTGGCGACCCTCCACTTTGACTCGTGACCAGCTGGAAGAACGACGGC
TGTATGCTCAGCAGCTCCTCGCCGCAGGTGAGGTCAATGCCAAGGAGATCGCGGCTTCGGTCGGCGTTTCCGAAAGTACAGTCCGCACCTGGAAACAGCG
TCTCCGAGAACGAGGCAGCCTCCAGGCCACCCGAGCGGCTGGGCCTTCACCGCGCCTGAGGCCAGAACAGCGCACGCAACTGGGGGAGATCCTGCGCGCA
GGACCGCTGGCCGCCGGTTATCCCGACGCGCGTTGGACGACCTCCCGCGTGCGGGAGGTCATTGGCCTGCAATTTGAGGTGTGGTACCACGCCGATCACG
TCAGGAAGTTGCTTCACCAGCTTGGCTTCAGTCCTCAGAAGCCAGAATCGCGTGCCTTGGAACGCGATGAGCAGGCGGTTCAGACCTGGGTCGAGCAGAC
GCTCCCCAGCTTGAAAAAAAAAGGTTGAGCAGGGTATGACTCTCGTCTTCCTCGATGAAAGTGGCTTCAGCCTGAAACCGACGGTGACCCGGACTTGGGC
CGTCCGAGGACAAACGCCGATCATCACGGCCAAAGCCAGTTGGGACAAGCTCTCGACTATTGGTGCGATAACGACTACGGGCCAGTTCTTACAACAGACC
TACCCAGGCGCCATTCGAGGCCAGCAGGTCGTGGCGTTCTGCCGACATCTCCTCCGCCACGTCCAAGGCAAGCTCGTTGTGTTGATGGACAACGCTCGTA
TCCACAAAACGAAGGCCCTGAGGGCCTTCGTCGAGCAGCAGCCCCGTCTTACCGTCGAGTATCTCCCGCCCTACGCTCCTGAACTCAATCCCATTGAGCG
GGTGTGGGCCTACATAAAAGGATCAGTCCTCGGAAACTTCTGCCCCAAAACTGTCGGTGAGTTGAAGGCAAGGCTAAACTCCGCGTGGCAACGTGTTCGT
TACATTCGGCTTCCCCAGCGCCTTACTCACCGCTACTGTTCGTCTCCAACCTAAGCCGGAGTCAA
TGTATGCTCAGCAGCTCCTCGCCGCAGGTGAGGTCAATGCCAAGGAGATCGCGGCTTCGGTCGGCGTTTCCGAAAGTACAGTCCGCACCTGGAAACAGCG
TCTCCGAGAACGAGGCAGCCTCCAGGCCACCCGAGCGGCTGGGCCTTCACCGCGCCTGAGGCCAGAACAGCGCACGCAACTGGGGGAGATCCTGCGCGCA
GGACCGCTGGCCGCCGGTTATCCCGACGCGCGTTGGACGACCTCCCGCGTGCGGGAGGTCATTGGCCTGCAATTTGAGGTGTGGTACCACGCCGATCACG
TCAGGAAGTTGCTTCACCAGCTTGGCTTCAGTCCTCAGAAGCCAGAATCGCGTGCCTTGGAACGCGATGAGCAGGCGGTTCAGACCTGGGTCGAGCAGAC
GCTCCCCAGCTTGAAAAAAAAAGGTTGAGCAGGGTATGACTCTCGTCTTCCTCGATGAAAGTGGCTTCAGCCTGAAACCGACGGTGACCCGGACTTGGGC
CGTCCGAGGACAAACGCCGATCATCACGGCCAAAGCCAGTTGGGACAAGCTCTCGACTATTGGTGCGATAACGACTACGGGCCAGTTCTTACAACAGACC
TACCCAGGCGCCATTCGAGGCCAGCAGGTCGTGGCGTTCTGCCGACATCTCCTCCGCCACGTCCAAGGCAAGCTCGTTGTGTTGATGGACAACGCTCGTA
TCCACAAAACGAAGGCCCTGAGGGCCTTCGTCGAGCAGCAGCCCCGTCTTACCGTCGAGTATCTCCCGCCCTACGCTCCTGAACTCAATCCCATTGAGCG
GGTGTGGGCCTACATAAAAGGATCAGTCCTCGGAAACTTCTGCCCCAAAACTGTCGGTGAGTTGAAGGCAAGGCTAAACTCCGCGTGGCAACGTGTTCGT
TACATTCGGCTTCCCCAGCGCCTTACTCACCGCTACTGTTCGTCTCCAACCTAAGCCGGAGTCAA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
477 bp | 158 aa | 52 | 528 | + | No |
Description : First part of the transposase
ORF sequence :
MWRPSTLTRDQLEERRLYAQQLLAAGEVNAKEIAASVGVSESTVRTWKQRLRERGSLQATRAAGPSPRLRPEQRTQLGEILRAGPLAAGYPDARWTTSRV
REVIGLQFEVWYHADHVRKLLHQLGFSPQKPESRALERDEQAVQTWVEQTLPSLKKKG
REVIGLQFEVWYHADHVRKLLHQLGFSPQKPESRALERDEQAVQTWVEQTLPSLKKKG
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
540 bp | 179 aa | 515 | 1054 | + | No |
Description : Second part of the transposase
ORF sequence :
KKKVEQGMTLVFLDESGFSLKPTVTRTWAVRGQTPIITAKASWDKLSTIGAITTTGQFLQQTYPGAIRGQQVVAFCRHLLRHVQGKLVVLMDNARIHKTK
ALRAFVEQQPRLTVEYLPPYAPELNPIERVWAYIKGSVLGNFCPKTVGELKARLNSAWQRVRYIRLPQRLTHRYCSSPT
ALRAFVEQQPRLTVEYLPPYAPELNPIERVWAYIKGSVLGNFCPKTVGELKARLNSAWQRVRYIRLPQRLTHRYCSSPT
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1003 bp | 333 aa | 52 | 1054 | + | Yes |
Chemistry : DDE
ORF sequence :
MWRPSTLTRDQLEERRLYAQQLLAAGEVNAKEIAASVGVSESTVRTWKQRLRERGSLQATRAAGPSPRLRPEQRTQLGEILRAGPLAAGYPDARWTTSRV
REVIGLQFEVWYHADHVRKLLHQLGFSPQKPESRALERDEQAVQTWVEQTLPSLKKKVEQGMTLVFLDESGFSLKPTVTRTWAVRGQTPIITAKASWDKL
STIGAITTTGQFLQQTYPGAIRGQQVVAFCRHLLRHVQGKLVVLMDNARIHKTKALRAFVEQQPRLTVEYLPPYAPELNPIERVWAYIKGSVLGNFCPKT
VGELKARLNSAWQRVRYIRLPQRLTHRYCSSPT
REVIGLQFEVWYHADHVRKLLHQLGFSPQKPESRALERDEQAVQTWVEQTLPSLKKKVEQGMTLVFLDESGFSLKPTVTRTWAVRGQTPIITAKASWDKL
STIGAITTTGQFLQQTYPGAIRGQQVVAFCRHLLRHVQGKLVVLMDNARIHKTKALRAFVEQQPRLTVEYLPPYAPELNPIERVWAYIKGSVLGNFCPKT
VGELKARLNSAWQRVRYIRLPQRLTHRYCSSPT
Blast result :
Comments
There are 3 copies in the genome. ISDra3 is 88% aa similar to ISDge7.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] White,O., Eisen,J.A., Heidelberg,J.F., Hickey,E.K., Peterson,J.D., Dodson,R.J., Haft,D.H., Gwinn,M.L., Nelson,W.C., Richardson,D.L., Moffat,K.S., Qin,H., Jiang,L., Pamphile,W., Crosby,M., Shen,M., Vamathevan,J.J., Lam,P., McDonald,L., Utterback,T., Zalewski,C., Makarova,K.S., Aravind,L., Daly,M.J., Minton,K.W., Fleischmann,R.D., Ketchum,K.A., Nelson,K.E., Salzberg,S., Smith,H.O., Venter,J.C. and Fraser,C.M. (1999) GenBank Direct Submission