ISPa8
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas sp. LM14 Pseudomonas pseudoalcaligenes CECT 5344 Pseudomonas pseudoalcaligenes |
DNA section
IS Length : 1324 bp
Ends
IR Length : 19
IRL : CAGCCTGTTGAAATTCGCCTACGGCCTGTCTGACTTTGCCGTGTAAGGTT
IRR : CAGCCTGTTGAAATTCGCCGCGATGATTACATCCACTGCTTCTGGCAGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGGCTGTCC | TTAG | GCAACAGGCC | 4 |
| TATGATTGAG | CTAA | ACGATGATTA | 4 |
DNA sequence
CAGCCTGTTGAAATTCGCCTACGGCCTGTCTGACTTTGCCGTGTAAGGTTTACGATATCGGCTCCAATCTGCCGGGAACCCCGATTTCCATGCGTGGCGC
CGATATCACTCAGGAATCCTTGTTCACTGTCGCCAAGCTCGATGACTTCGTGCCAGCGACTCACCCCCTGCGCGCCATCCGCAAGCTGGCAGACACCGCT
CTGCAGCGGATGAGCGCCCTGTTCGACACCTTGTATGCCGACACCGGCCGCGCCTCGATCGCCCCCGAGAAGNTGATGCGGGCGCAGTTGCTGCAGTTGT
TCTACTCGCTGCGCAGCGAGCGGATGCTGATGGAACAGCTGGGCTACAACCTGCTGTTCCGCTGGTTCGTCGGCCTGGCCATTGATGATCCGGTGTGGGA
TCACTCGGTCTTCTCGAAGAATCGTGACCGCCTCAATGCCCAGGCCGTGGCCACCGAATTTCTGAACGAGGTGGTACGCCTGGCGGAGAAGCAGGGGCTG
ATCTCGGATGAGCACTTCTCGGTCGACGGCACATTGCTGCAAGCCTGGGCCTCTCAGAAGAGTTTCCGTCCCAAGGACGATACGCCAGACGACCCGGGCC
CCGGCCCGCGCAATGCCCAGCCAGACTTCAAGGGGCACAAGCGCAGCAACGACACCCACGCCTCGACCAGCGATCCGGATGCCCGGCTGTACCGCAAGAG
CCACAACACCGGCGCGCAGCTGAGTTACATGGGGCATGTACTGATGGAACACCGCAGTGGCCTGGTGCGCAATGCTCATGTGACTCTGGCCGGTGGCCAT
GGCGAACGCGAAGCCGCGCTGGCGATGCTGAGCAAGCTGGGTGGTCGCCGTCGACGCACCTTGGCCGCGGACAAGGCTTACGACACGGCAGACTTCGTGG
CCGCCTGTCGTGCCCTCGGCGTGACACCCCATGTTGCGCAGAACACCAATGGTCGGCGCAGCGCCATCGATGGCCGTACCACGCGCCACGCCGGCTATGG
TCTGAGCCTGAAGATTCGCGCCTGGATCGAAACCCACTTCGGCTGGCTCAAGGCGGCGGCCGGGCTGCGCCAGGTGAAGCAACGCGGACTTGAGCGTGTC
GACGCGCTGTTCCAGCTGGCCATGGCGGCAAGCAACCTGGTGCGCCTGCCGAAGCTGCTGGCTACAGGCTCAATGGCATGAGCGCGTTGACGGGGAAAGT
GCGCCTGGAGCACGCCAAATCGGGCTATTTCCATTGATTGGCGCCCTAAACGGCAAGTAAACGAGCGCGACCGCTGCTGCCAGAAGCAGTGGATGTAATC
ATCGCGGCGAATTTCAACAGGCTG
CGATATCACTCAGGAATCCTTGTTCACTGTCGCCAAGCTCGATGACTTCGTGCCAGCGACTCACCCCCTGCGCGCCATCCGCAAGCTGGCAGACACCGCT
CTGCAGCGGATGAGCGCCCTGTTCGACACCTTGTATGCCGACACCGGCCGCGCCTCGATCGCCCCCGAGAAGNTGATGCGGGCGCAGTTGCTGCAGTTGT
TCTACTCGCTGCGCAGCGAGCGGATGCTGATGGAACAGCTGGGCTACAACCTGCTGTTCCGCTGGTTCGTCGGCCTGGCCATTGATGATCCGGTGTGGGA
TCACTCGGTCTTCTCGAAGAATCGTGACCGCCTCAATGCCCAGGCCGTGGCCACCGAATTTCTGAACGAGGTGGTACGCCTGGCGGAGAAGCAGGGGCTG
ATCTCGGATGAGCACTTCTCGGTCGACGGCACATTGCTGCAAGCCTGGGCCTCTCAGAAGAGTTTCCGTCCCAAGGACGATACGCCAGACGACCCGGGCC
CCGGCCCGCGCAATGCCCAGCCAGACTTCAAGGGGCACAAGCGCAGCAACGACACCCACGCCTCGACCAGCGATCCGGATGCCCGGCTGTACCGCAAGAG
CCACAACACCGGCGCGCAGCTGAGTTACATGGGGCATGTACTGATGGAACACCGCAGTGGCCTGGTGCGCAATGCTCATGTGACTCTGGCCGGTGGCCAT
GGCGAACGCGAAGCCGCGCTGGCGATGCTGAGCAAGCTGGGTGGTCGCCGTCGACGCACCTTGGCCGCGGACAAGGCTTACGACACGGCAGACTTCGTGG
CCGCCTGTCGTGCCCTCGGCGTGACACCCCATGTTGCGCAGAACACCAATGGTCGGCGCAGCGCCATCGATGGCCGTACCACGCGCCACGCCGGCTATGG
TCTGAGCCTGAAGATTCGCGCCTGGATCGAAACCCACTTCGGCTGGCTCAAGGCGGCGGCCGGGCTGCGCCAGGTGAAGCAACGCGGACTTGAGCGTGTC
GACGCGCTGTTCCAGCTGGCCATGGCGGCAAGCAACCTGGTGCGCCTGCCGAAGCTGCTGGCTACAGGCTCAATGGCATGAGCGCGTTGACGGGGAAAGT
GCGCCTGGAGCACGCCAAATCGGGCTATTTCCATTGATTGGCGCCCTAAACGGCAAGTAAACGAGCGCGACCGCTGCTGCCAGAAGCAGTGGATGTAATC
ATCGCGGCGAATTTCAACAGGCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1092 bp | 363 aa | 90 | 1181 | + | No |
Chemistry : DDE
ORF sequence :
MRGADITQESLFTVAKLDDFVPATHPLRAIRKLADTALQRMSALFDTLYADTGRASIAPEKXMRAQLLQLFYSLRSERMLMEQLGYNLLFRWFVGLAIDD
PVWDHSVFSKNRDRLNAQAVATEFLNEVVRLAEKQGLISDEHFSVDGTLLQAWASQKSFRPKDDTPDDPGPGPRNAQPDFKGHKRSNDTHASTSDPDARL
YRKSHNTGAQLSYMGHVLMEHRSGLVRNAHVTLAGGHGEREAALAMLSKLGGRRRRTLAADKAYDTADFVAACRALGVTPHVAQNTNGRRSAIDGRTTRH
AGYGLSLKIRAWIETHFGWLKAAAGLRQVKQRGLERVDALFQLAMAASNLVRLPKLLATGSMA
PVWDHSVFSKNRDRLNAQAVATEFLNEVVRLAEKQGLISDEHFSVDGTLLQAWASQKSFRPKDDTPDDPGPGPRNAQPDFKGHKRSNDTHASTSDPDARL
YRKSHNTGAQLSYMGHVLMEHRSGLVRNAHVTLAGGHGEREAALAMLSKLGGRRRRTLAADKAYDTADFVAACRALGVTPHVAQNTNGRRSAIDGRTTRH
AGYGLSLKIRAWIETHFGWLKAAAGLRQVKQRGLERVDALFQLAMAASNLVRLPKLLATGSMA
Blast result :
Comments
ISPa8 is 66% aa similar to ISRme1.
An isoform of ISPa8 has been identified in Pseudomonas sp. LM14 (KJ920389) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis ISPa8 is present in 9 copies in the genome of Pseudomonas sp. LM14. Homologous sequences were also detected by DNA hybridization in Pseudomonas mendocina strain LM7, Pseudomonas sp. LM8.
File updated the January 22 2015 with the information provided by Szuplewska M.
An isoform of ISPa8 has been identified in Pseudomonas sp. LM14 (KJ920389) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis ISPa8 is present in 9 copies in the genome of Pseudomonas sp. LM14. Homologous sequences were also detected by DNA hybridization in Pseudomonas mendocina strain LM7, Pseudomonas sp. LM8.
File updated the January 22 2015 with the information provided by Szuplewska M.
References
1] Ruimy, R. (2002) Direct submission
2] Szuplewska, M., and Bartosik, D. (2014) Direct submission.
2] Szuplewska, M., and Bartosik, D. (2014) Direct submission.