ISAli15

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum 4B plasmid p2AZO 549
DNA section
IS Length : 2658 bp

Ends


IR Length : 16/21

IRL : GTAAGCGTTGCGTGACGACACATCCGACATGACCCTGGTTCATGAAATAT
IRR : GTAAGCGGGGCGTGATTGCACCGGATCAGGCGTTGACGGCGGGCTTCCAG

Insertion site


Left flankDirect repeatRight flankDR Length
TATCGGAAGCCCGAGTTGCTGGCGAGGGCAAGGGGCTGGCGATCGT0

DNA sequence

GTAAGCGTTGCGTGACGACACATCCGACATGACCCTGGTTCATGAAATATTCCGCTGGTCGCAGCTCAGCGGGGTAAGAGGATGTCGGGTCAGGATCTTG
AGAGCTCTTGGCGCGGTCATGTCGAGGTCTGGAAGGGTAGCGGACTGACGCTGAAAGCCTATGCTGAAGCCCATGGCATTGGGCGGTGGGCTCTGGGGCG
GTGGTCGCGCCGCTTTGCCGCTGCGCAGGAGGTGAGCGCTCGCCGTCATAGGGATAGTCCTGGTGCTGTCCCTATGACGGCGAGCGCTCCCTTGGTGATG
GAGATCGTCGAGGCGGCCCCCCGCCGGCGGTATTGGTCACAGGCGGAGAAAGAGCAGCTGGTGGCGGAAACCTGCGAGCCTGGCGTGAGCGTGTCGCTGG
TCGCCCGCCGGCGCGGGGTCGATCCCAGCCAGCTGTTCCGCTGGCGCCGCCGGATGCTCACTCCGGCCGAGCCGACGCCCATCTTCGCGCCGGTGGAGGT
GGCGGACACCACGTCCGCTCTTCCCCTCACGCCGCCAACTCCATGCGGTGCCGGCATGATCGAGATCGAGTTGGCCGGCGGACGGCGGCTGCGGGTCGGG
CGGGATGTCGATGCCGAAGCATTGCGCCGGGTGATCAGCGTGCTGGAGCGGCCATGATGCTGTCGATCCCGGCCGGTGTGCGCATTTACCTGGCGCTGGA
GCCCTGCGACATGCGCCGCGGCTTCGACGGACTGGCCCTGCTGGTGCAGCAGGCGCTGGGCAAGGATCCGTTCTGCGGCCACCTCTACATCTTCCGAGGG
AAAGGGGCTGGACGCGTGAAAATTCTCTACGCCGATCAGAACGGCATGTGCCTGTTCGCCAAGAGGCTGGAGCGTGGCCGTTTCGTTTGGCCAACGACGC
GGACGCCGGGCGGCACGGTGGTACTGACGCCGGCGGAGTTATCCCTGCTTCTGGAGGGGCTGGACTGGCGTCACACCGTGCCGGTGTCGCGGCCGACCGC
AGCGGGATGAATGGAAGAAAAAGTGAGGAAAACCGGGGGCTTGGCTGGCCTGAGACCGTGAGCCGAGGTACAATCGGCCATGCGCTTCGACCTCGACAAC
CTGCCCGCCGATCCGGCCCTGCTGCAGCAGATGGTGCGCGATTTGGCCGAGGTTGTGGAGCGCCAGAAAGCCGATCTGGCGGAGTTGGAGGCTCTGCGTC
AGCAACTCCGGCAGATGCAGCGCACCGTCTTCGGGCGCCGCTCCGAACGGCTCGATCCCGATCAGCTCGATCTCGGGTTGGAGGAGCTGGAGGCGGACAT
CGCCCGGGTCGAGGCCAAACTCGCAGCCCCTGAACTGGAACCGTCGGCCCCTGCGGAACCGCCCAGCCGTGGCCGCGACTTGCCCGATGATCTGCCGCAT
CACGATCTCACGCTCGAGCCGCCCGGGCTGGAGTCCGGCGCCGGGGTTTGCCCCTGCTGCGGCGGGGCTTTGCATGAGGCCGGCGAGACGGTGGCCCGGA
TGATCGACTACGTGCCGGCCCAGGTGCGGGTGCTGCGCATCCGCCGGCCCAAATACGCCTGCCGCGGCTGCGGCTCGCTGCATCAGGCGCCGGCTCCGGA
GAAGCCGATCGCCAAAGGCATGGCGACGCCGGCGATGCTGGCGCACGTCATCACCAGCCGCTACTGCGATCATCTGCCCTTCTACCGCCAGGCGCAGATC
CTGGCCCGCAACGGCTTCCCCGTCGACCGCTCGGTTCTGGCGGGCTGGGCGGGACAGGCGTGCTGGTGGCTGGAGGCGCTGCATGAGGAGCTGGCCAAGG
CCCTGTTCGCCTCGGCCAAGCTGTTCGCCGACGACACGCCAATGCCGACGCTGGCGCCGGGAACCGGGCGGGTGAAGACCGGACGGCTGTGGGCTTATGC
CCGCGATGACCGGCCCTGGCAGGGGCTGGACCCGCCGGCGGTGATTTACGCCTACACCAGCGATCGCAAAGGCGAACGCCCAGCTGCCCATCTGCGGGAG
TTCCGCGGGGTGCTGCAGGTGGACGGTTATGCCGGGTTCGAACGGCTGGCTGCCAGCAACCGGGTCGTGCTGGCGGCGTGCTGGAGCCATGCGCGTCGAC
GCTTCTACGACTTGGCCGAGGCCGGCTCGCCGGCCGCCGCCGAGGCGCTGCGCCGCATCGCCCGGCTCTACGCGATCGAGGAGCGCATCCGCGGCCGCAC
GGCCGATGAGCGCCGGCAGCTTCGGCAAGCCGAAGCCGCTTCCCAGGTGGCGGAGCTGAAGGCGTGGCTGGAGCACGAACTTCCCCGCCTGCCGGGCCGC
TCCAAGCTGGCCGAGGCGATCCGCTATGCGCTCGGCCGCTGGAAGGCGCTGTGCGTCTACCTGGATGACGGCCGGGTGGAGATGGATACCAACACGGTGG
AGCGCTGCATCAGACCGGTAGCCCTTGGCCGGAAGAATAGCCTCTTCGCCGGCTCGGAGGGCGGCGGCCATCGCTGGGCGGTGATCGCCTCGCTGGTTGA
GACCTGCAAGCTCAACGCTGTCGAGCCGTTCGCCTACCTGCGCGACGTCCTGGAGCGCATGGTGAACGGCTACCCCGCCGCACGCCTCGCCGATATCCTC
CCCTGAAACTGGAAGCCCGCCGTCAACGCCTGATCCGGTGCAATCACGCCCCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa274657+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTASAPLVMEIVEAAPRRRYWSQAEKEQLVAETCEPGVSVSLVARRRGVDPSQLFRWRRRMLTPAEPTPIFAPVEVADTTSALPLTPPTPCGAGMIEIEL
AGGRRLRVGRDVDAEALRRVISVLERP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
357 bp118 aa6541010+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MMLSIPAGVRIYLALEPCDMRRGFDGLALLVQQALGKDPFCGHLYIFRGKGAGRVKILYADQNGMCLFAKRLERGRFVWPTTRTPGGTVVLTPAELSLLL
EGLDWRHTVPVSRPTAAG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1527 bp508 aa10802606+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRFDLDNLPADPALLQQMVRDLAEVVERQKADLAELEALRQQLRQMQRTVFGRRSERLDPDQLDLGLEELEADIARVEAKLAAPELEPSAPAEPPSRGRD
LPDDLPHHDLTLEPPGLESGAGVCPCCGGALHEAGETVARMIDYVPAQVRVLRIRRPKYACRGCGSLHQAPAPEKPIAKGMATPAMLAHVITSRYCDHLP
FYRQAQILARNGFPVDRSVLAGWAGQACWWLEALHEELAKALFASAKLFADDTPMPTLAPGTGRVKTGRLWAYARDDRPWQGLDPPAVIYAYTSDRKGER
PAAHLREFRGVLQVDGYAGFERLAASNRVVLAACWSHARRRFYDLAEAGSPAAAEALRRIARLYAIEERIRGRTADERRQLRQAEAASQVAELKAWLEHE
LPRLPGRSKLAEAIRYALGRWKALCVYLDDGRVEMDTNTVERCIRPVALGRKNSLFAGSEGGGHRWAVIASLVETCKLNAVEPFAYLRDVLERMVNGYPA
ARLADILP

 

Blast result :
Comments
ISAli15 is 53% (ORFA) and 74% (ORFB) aa similar to IS66 and 64% (ORFC) to ISPre3.
References
1] ISfinder annotation (2008)