ISPlu14
- Family IS5
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005126 | ND | Photorhabdus luminescens | Photorhabdus luminescens subsp. laumondii TTO1 |
DNA section
IS Length : 1582 bp
Ends
IR Length : 11/15
IRL : CAGATTATTGACAAAGTGCTGGTTTTTTATCCGGCATTTTGTTTTAATTA
IRR : CAGACCGCTGACAAACCCCATTGAAAATAATGGGGTTTGTTCCTTTCATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAATGCTAT | AGGGCATCTCTATGCCCT | TTAATTATTC | 18 |
| AGTGATTTAA | GACACAGCATTACGCTGTGTCT | TAAATATGTT | 22 |
DNA sequence
CAGATTATTGACAAAGTGCTGGTTTTTTATCCGGCATTTTGTTTTAATTAAGTTCCGTTCACTCAACGGAGCTTGCCACCATGTTAAGACCCCCCCCTCT
CCAGACTTTCCCGCCAGAAACGCTCTCGCTTGACGAGCTGGTCCCTAAAAATCATTTGGTCCGTCAAGTCGATGCTGCCATTGATTTCGAATTTATCCGC
GAATTAGTCGCCCCGTTGTATTGCCACAATAATGGCCGTCCTGCTATCGATCCGGTCATGCTGATTAAAATGATGTTACTCGGCTACTTGTTTGGCGTCC
CCAGTGAACGCCGTCTGGTACAGGAAATTCAGGTTAATGTGGCTTACCGCTGGTTTCTGCGCCTCGGACTGACAGAAAAGGTCCCTGATGCCTCAACCCT
GAGCCAGAACCGCCGCCGCCGTTTCAATCATACCGCGGTCTTCCAACAGATTTTTGACCACATTGTTGAACAGGCGATAGCCAAAGGATTTGTGGGCGGA
CACGTACTCTATACCGACAGCACCCACCTGAAAGCCAGTGCCAATCCGCATAAATCGGAGAATGTCATGCGGTCGGTTCCCCCCGGCGCCTATGTTGACG
CACTGGATAAAGCCGTCACTCAAGACCGGGCGGCAGCTGGAAAAAAAGCCTGAAGCCAGCCCTAAAGGAACGGCAGAGAAAGACGAAGGTCAGTACCGTC
GATCCGGAAAGCGGGTTTATGCACCGGACCAACAAACCCAGGGGGTTCTTTTACCTTGACCACCGTACCGTGGATGGGCAAGTCGGTATTATCACCGATA
CCTACGCCACACCGGGTAATGTCCACGACAGCCAGCCTTTTATCAAGCGTTTGACGCGTCAGTTAGAGCGTTTTGCTCTCAATCCCCTCGCGGTTGGGTT
GGATGCGGGCTATTTTACGGCCCCGGTTTGTTATCTGACGGAACAGTTAGGCATTATCCCCATCATAGGTTATCGCCGGCCGAATAAGGGACCAAATACC
TTTCAGAAGAAACATTTCACCTATGACCAGCTACAGGATTGTTATGTCTGCCCGCAGGGAGAAAAACTGATTTATACCACAACCGACCGTCAGGGATACC
GGCATTATCAGGCCCCGGCGGCTATCTGCCAGTGTTGCCCGCAGCGTGATGCCTGCACGCACGCGAAAGGCGGAAAAACGATAACCCGTCATATCTGGGA
GGCCAGTAAAGAGCAGGCCAGAGAAAACCGGCTAACGGAATGGGGTAAAAAAGTTTACAAGCGACGAAAGGAAACAATAGAGCGCAGTTTTGCGGATGCG
AAGCAACATCACGGGCACCGTTATGCCCATTTTCGCGGGTTGCAGAAGGTGCAAATACAGTGTCTTTTGGCGGCAACTGCCCAAAATATCAAGAAGATAG
CCTTGCTGGTCGCGATGCTTTGTTGTTTTTATCTCTGGAGGGCGAGCCAGTCAATGCCCGCGAAGCGAAAATAACGCCCTGACGGGCGATTGAATCGGGT
CAATGAGGAAAATGAGTTGTATTATGATTCGTAATGAAAGGAACAAACCCCATTATTTTCAATGGGGTTTGTCAGCGGTCTG
CCAGACTTTCCCGCCAGAAACGCTCTCGCTTGACGAGCTGGTCCCTAAAAATCATTTGGTCCGTCAAGTCGATGCTGCCATTGATTTCGAATTTATCCGC
GAATTAGTCGCCCCGTTGTATTGCCACAATAATGGCCGTCCTGCTATCGATCCGGTCATGCTGATTAAAATGATGTTACTCGGCTACTTGTTTGGCGTCC
CCAGTGAACGCCGTCTGGTACAGGAAATTCAGGTTAATGTGGCTTACCGCTGGTTTCTGCGCCTCGGACTGACAGAAAAGGTCCCTGATGCCTCAACCCT
GAGCCAGAACCGCCGCCGCCGTTTCAATCATACCGCGGTCTTCCAACAGATTTTTGACCACATTGTTGAACAGGCGATAGCCAAAGGATTTGTGGGCGGA
CACGTACTCTATACCGACAGCACCCACCTGAAAGCCAGTGCCAATCCGCATAAATCGGAGAATGTCATGCGGTCGGTTCCCCCCGGCGCCTATGTTGACG
CACTGGATAAAGCCGTCACTCAAGACCGGGCGGCAGCTGGAAAAAAAGCCTGAAGCCAGCCCTAAAGGAACGGCAGAGAAAGACGAAGGTCAGTACCGTC
GATCCGGAAAGCGGGTTTATGCACCGGACCAACAAACCCAGGGGGTTCTTTTACCTTGACCACCGTACCGTGGATGGGCAAGTCGGTATTATCACCGATA
CCTACGCCACACCGGGTAATGTCCACGACAGCCAGCCTTTTATCAAGCGTTTGACGCGTCAGTTAGAGCGTTTTGCTCTCAATCCCCTCGCGGTTGGGTT
GGATGCGGGCTATTTTACGGCCCCGGTTTGTTATCTGACGGAACAGTTAGGCATTATCCCCATCATAGGTTATCGCCGGCCGAATAAGGGACCAAATACC
TTTCAGAAGAAACATTTCACCTATGACCAGCTACAGGATTGTTATGTCTGCCCGCAGGGAGAAAAACTGATTTATACCACAACCGACCGTCAGGGATACC
GGCATTATCAGGCCCCGGCGGCTATCTGCCAGTGTTGCCCGCAGCGTGATGCCTGCACGCACGCGAAAGGCGGAAAAACGATAACCCGTCATATCTGGGA
GGCCAGTAAAGAGCAGGCCAGAGAAAACCGGCTAACGGAATGGGGTAAAAAAGTTTACAAGCGACGAAAGGAAACAATAGAGCGCAGTTTTGCGGATGCG
AAGCAACATCACGGGCACCGTTATGCCCATTTTCGCGGGTTGCAGAAGGTGCAAATACAGTGTCTTTTGGCGGCAACTGCCCAAAATATCAAGAAGATAG
CCTTGCTGGTCGCGATGCTTTGTTGTTTTTATCTCTGGAGGGCGAGCCAGTCAATGCCCGCGAAGCGAAAATAACGCCCTGACGGGCGATTGAATCGGGT
CAATGAGGAAAATGAGTTGTATTATGATTCGTAATGAAAGGAACAAACCCCATTATTTTCAATGGGGTTTGTCAGCGGTCTG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 573 bp | 190 aa | 81 | 653 | + | No |
Description : First part of the transposase
ORF sequence :
MLRPPPLQTFPPETLSLDELVPKNHLVRQVDAAIDFEFIRELVAPLYCHNNGRPAIDPVMLIKMMLLGYLFGVPSERRLVQEIQVNVAYRWFLRLGLTEK
VPDASTLSQNRRRRFNHTAVFQQIFDHIVEQAIAKGFVGGHVLYTDSTHLKASANPHKSENVMRSVPPGAYVDALDKAVTQDRAAAGKKA
VPDASTLSQNRRRRFNHTAVFQQIFDHIVEQAIAKGFVGGHVLYTDSTHLKASANPHKSENVMRSVPPGAYVDALDKAVTQDRAAAGKKA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 864 bp | 287 aa | 611 | 1474 | + | No |
Description : Second part of the transposase
ORF sequence :
SRHSRPGGSWKKSLKPALKERQRKTKVSTVDPESGFMHRTNKPRGFFYLDHRTVDGQVGIITDTYATPGNVHDSQPFIKRLTRQLERFALNPLAVGLDAG
YFTAPVCYLTEQLGIIPIIGYRRPNKGPNTFQKKHFTYDQLQDCYVCPQGEKLIYTTTDRQGYRHYQAPAAICQCCPQRDACTHAKGGKTITRHIWEASK
EQARENRLTEWGKKVYKRRKETIERSFADAKQHHGHRYAHFRGLQKVQIQCLLAATAQNIKKIALLVAMLCCFYLWRASQSMPAKRK
YFTAPVCYLTEQLGIIPIIGYRRPNKGPNTFQKKHFTYDQLQDCYVCPQGEKLIYTTTDRQGYRHYQAPAAICQCCPQRDACTHAKGGKTITRHIWEASK
EQARENRLTEWGKKVYKRRKETIERSFADAKQHHGHRYAHFRGLQKVQIQCLLAATAQNIKKIALLVAMLCCFYLWRASQSMPAKRK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1394 bp | 464 aa | 81 | 1474 | + | Yes |
Chemistry : DDE
ORF sequence :
MLRPPPLQTFPPETLSLDELVPKNHLVRQVDAAIDFEFIRELVAPLYCHNNGRPAIDPVMLIKMMLLGYLFGVPSERRLVQEIQVNVAYRWFLRLGLTEK
VPDASTLSQNRRRRFNHTAVFQQIFDHIVEQAIAKGFVGGHVLYTDSTHLKASANPHKSENVMRSVPPGAYVDALDKAVTQDRAAAGKKSLKPALKERQR
KTKVSTVDPESGFMHRTNKPRGFFYLDHRTVDGQVGIITDTYATPGNVHDSQPFIKRLTRQLERFALNPLAVGLDAGYFTAPVCYLTEQLGIIPIIGYRR
PNKGPNTFQKKHFTYDQLQDCYVCPQGEKLIYTTTDRQGYRHYQAPAAICQCCPQRDACTHAKGGKTITRHIWEASKEQARENRLTEWGKKVYKRRKETI
ERSFADAKQHHGHRYAHFRGLQKVQIQCLLAATAQNIKKIALLVAMLCCFYLWRASQSMPAKRK
VPDASTLSQNRRRRFNHTAVFQQIFDHIVEQAIAKGFVGGHVLYTDSTHLKASANPHKSENVMRSVPPGAYVDALDKAVTQDRAAAGKKSLKPALKERQR
KTKVSTVDPESGFMHRTNKPRGFFYLDHRTVDGQVGIITDTYATPGNVHDSQPFIKRLTRQLERFALNPLAVGLDAGYFTAPVCYLTEQLGIIPIIGYRR
PNKGPNTFQKKHFTYDQLQDCYVCPQGEKLIYTTTDRQGYRHYQAPAAICQCCPQRDACTHAKGGKTITRHIWEASKEQARENRLTEWGKKVYKRRKETI
ERSFADAKQHHGHRYAHFRGLQKVQIQCLLAATAQNIKKIALLVAMLCCFYLWRASQSMPAKRK
Blast result :
Comments
ISPlu14 is 73% aa similar to ISBma2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There are 2 complete and 11 partial copies in the genome.
There are 2 complete and 11 partial copies in the genome.
References
1] Duchaud,E., Rusniok,C., Frangeul,L., Buchrieser,C., Taourit,S., Bocs,S., Boursaux-Eude,C., Chandler,M., Dassa,E., Derose,R.,Derzelle,S., Freyssinet,G., Gaudriault,S., Givaudan,A., Glaser,P.,Medigue,C., Lanois,A., Powell,K., Siguier,P., Wingate,V.,Zouine,M., Boemare,N., Danchin,A. and Kunst,(2003) F.Nat. Biotechnol