ISPlu7
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005126 | ND | Photorhabdus luminescens | Photorhabdus luminescens subsp. laumondii TTO1 |
DNA section
IS Length : 921 bp
Ends
IR Length : 13/16
IRL : GGGTGTGTCCCGCAATAGGCTGAGATAGAATAATCTATGTGGCTCTGACT
IRR : GGTTATGTCCCTCAATTAAATTATGGCCTTAAGAAATAAGCGGATACAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAAATGCAC | TA | GTATATTTTT | 2 |
DNA sequence
GGGTGTGTCCCGCAATAGGCTGAGATAGAATAATCTATGTGGCTCTGACTTTCGGAAAAAACATGTTATGGCTCGTTACGACCTTCCTGATGAAGCATGG
GAGTTACTGCTCCCCTTATTGCCCCCTGAACATTCTCAACGGGCGGGACATCCTTATGTTGAACACCGCCGGGTTATCAACGGCATGTTTTGGATACTCT
GTTCCGGTGCCCCCTGGCGGGATTTACCCGAACGTTATGGTTCCTGGAAAACCGTCTATAATCGCTTTAATCGGTGGGCAAAATCGGGGCTCATTAACCT
CATTTTCAATAAGTTATTGTCAATACTGGATGAACAAGGTTTAGTTGATTGGTCAGCTATATCTCTGGATGGCAGTAACATTCGTGCGGCACATTGTGCG
GCGGGCGCCAAAAAAAACATCCCGATGTCACCGAAAATCATGGCCTGGGTCGCTCTCAGGGTGGTTATGGCTCCAAAATCCATCTGGCGGTCGATGGAAA
AGGCTATCCACTGAATGTGGTACTCAGTGCAGGTCAATGTCATGAAAGTCAATATGCAGTGCGCCTGCTGGATGGTCTGGGTATTCAGCGTAAAAACGGT
TTTATGAAACGTCGTTCAAAAGCGGTGATTGCTGATAAAGGTTATGCCAGCCACGCATTACGTAACCGGTTAAAAAGTCAGGGAATAAAAGTGATAATTC
CCTATAAATCAAATGAAAAAGCGCGGTCAGATGGACGGGTTAAATTCGACCATGAAACGTATCGAAGAAGAAATGTTGTAGAAAGATGTTTTGGTCGACT
CAAAGAGCATCGTCGCATCGCCACCCGTTATGAAAAGACAGCAAGAAATTATATCGCTATGGTGAAATTAGGTTGTATCCGCTTATTTCTTAAGGCCATA
ATTTAATTGAGGGACATAACC
GAGTTACTGCTCCCCTTATTGCCCCCTGAACATTCTCAACGGGCGGGACATCCTTATGTTGAACACCGCCGGGTTATCAACGGCATGTTTTGGATACTCT
GTTCCGGTGCCCCCTGGCGGGATTTACCCGAACGTTATGGTTCCTGGAAAACCGTCTATAATCGCTTTAATCGGTGGGCAAAATCGGGGCTCATTAACCT
CATTTTCAATAAGTTATTGTCAATACTGGATGAACAAGGTTTAGTTGATTGGTCAGCTATATCTCTGGATGGCAGTAACATTCGTGCGGCACATTGTGCG
GCGGGCGCCAAAAAAAACATCCCGATGTCACCGAAAATCATGGCCTGGGTCGCTCTCAGGGTGGTTATGGCTCCAAAATCCATCTGGCGGTCGATGGAAA
AGGCTATCCACTGAATGTGGTACTCAGTGCAGGTCAATGTCATGAAAGTCAATATGCAGTGCGCCTGCTGGATGGTCTGGGTATTCAGCGTAAAAACGGT
TTTATGAAACGTCGTTCAAAAGCGGTGATTGCTGATAAAGGTTATGCCAGCCACGCATTACGTAACCGGTTAAAAAGTCAGGGAATAAAAGTGATAATTC
CCTATAAATCAAATGAAAAAGCGCGGTCAGATGGACGGGTTAAATTCGACCATGAAACGTATCGAAGAAGAAATGTTGTAGAAAGATGTTTTGGTCGACT
CAAAGAGCATCGTCGCATCGCCACCCGTTATGAAAAGACAGCAAGAAATTATATCGCTATGGTGAAATTAGGTTGTATCCGCTTATTTCTTAAGGCCATA
ATTTAATTGAGGGACATAACC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 447 bp | 148 aa | 68 | 514 | + | No |
Description : First part of the transposase
ORF sequence :
MARYDLPDEAWELLLPLLPPEHSQRAGHPYVEHRRVINGMFWILCSGAPWRDLPERYGSWKTVYNRFNRWAKSGLINLIFNKLLSILDEQGLVDWSAISL
DGSNIRAAHCAAGAKKNIPMSPKIMAWVALRVVMAPKSIWRSMEKAIH
DGSNIRAAHCAAGAKKNIPMSPKIMAWVALRVVMAPKSIWRSMEKAIH
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 396 bp | 131 aa | 511 | 906 | + | No |
Description : Second part of the transposase
ORF sequence :
MNVVLSAGQCHESQYAVRLLDGLGIQRKNGFMKRRSKAVIADKGYASHALRNRLKSQGIKVIIPYKSNEKARSDGRVKFDHETYRRRNVVERCFGRLKEH
RRIATRYEKTARNYIAMVKLGCIRLFLKAII
RRIATRYEKTARNYIAMVKLGCIRLFLKAII
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 839 bp | 0 aa | 68 | 906 | + | Yes |
Chemistry : DDE
ORF sequence :
Blast result :
Comments
ISPlu7 is 81% (ORF1) and 83% (ORF2) aa similar to ISEhe2.
There are 4 complete copies and 3 partial copies in the genome.
There are 4 complete copies and 3 partial copies in the genome.
References
1] Duchaud,E., Rusniok,C., Frangeul,L., Buchrieser,C., Taourit,S., Bocs,S., Boursaux-Eude,C., Chandler,M., Dassa,E., Derose,R.,Derzelle,S., Freyssinet,G., Gaudriault,S., Givaudan,A., Glaser,P.,Medigue,C., Lanois,A., Powell,K., Siguier,P., Wingate,V.,Zouine,M., Boemare,N., Danchin,A. and Kunst,(2003) F.Nat. Biotechnol