ISP1
- Family ISL3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004567 | ND | Lactobacillus plantarum | Lactobacillus plantarum WCFS1 |
DNA section
IS Length : 1433 bp
Ends
IR Length : 8/9
IRL : GGGTCTATGATATTTAGTGTTGATGGATAAAATCCATCAACACTATTTTT
IRR : GGGTCTCTGTCAACCGGTGTTGATGAAGTTATATTTAGAATCCAATTCAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATCACGAGT | ATTAAAAA | CACCTATCCA | 8 |
DNA sequence
GGGTCTATGATATTTAGTGTTGATGGATAAAATCCATCAACACTATTTTTATGTACAACAAAAGGACATATCGTCCTGTGCTACAATCAAGTCGACCAAA
ACCAATTGAAAGCGAGTGTCAGATATGTCCCAAGACCATTCTACTAAAGTACTGCTCCAAATTAAAGACCCAAATATTACTGATTTAGTTGTTCAAGAAG
CTACTAACGGAAGCTTACAAGTGCTTGCGAAGCTAACGTATCCGGTTACTGTTTGCGCACGTTGTGGCAAACGTTCCATGGTTAAGAATGGCTTTAAGAC
CGTTCACATCAGAATTCCTAGAATTAGCGAACGGGTCACCATCCTTAACTTAAAGAAGCAGCGATTTATCTGTAAAAATTGTGGTAAAAGTAGTTTAACA
ACGACTACGCTGGTTAAAAAACATCATCAAATATCTGAAAACAGTCTTCATGCCATTGATCTATCATTAACTGAAGACCGTACGATGGCGAGTATTGCGG
CTCAATACAATGTCTCGACCAATACGATTAGTCGCCGAATAAAGTTGCTCGGACAAGGTACCCAACCAGCTTTCAACGGACTACCAACGATGCTTTGTAT
CGACGAGTTTCGTTCAACTGATAAACAAATGAGTTTTATTGCGGTGGATGCCCAAACCCACGACATCATTACAATATTACCAGGACGTAAGAATGCCGAT
ATTAAAGACTTCTTTCTCAATCATTATTCTAAACGTCATCGTGACCGCGTAACCCGGGTTGTGATGGACTTTAATTCTCAATATCAACCCGCGATTCGAA
CACTCTTTCCACACGCCAAACTGGTCGCGGATAACTTTCATCTTGTTCAAATGGCCTTACGATCTTTGAATCAAACTCGGGTCCAACTCATGAAACGCTT
TAACCCTAAGAGTCGTGAATACCGAGTGCTTAAATATTATTGGCGGTTATATTTAAAAGATTATGACAGCCTCGAAAAGCGCAAACCCCAATGGTTTGCG
CATCTTAAAGATCGCATGACACAAGAGCAATTAGTCGTAGCCGGTTTAAATCTAGACCAACAGTTTCGACAAACCTACCAAGCCGCACATGCCTTAGTTA
AGGCTTTTCAAAAGCGTGATTATCGATCCTTCAAAGCAGCACTAGGTCATGTCGAAAATATCAGTCCTCAGCTAATGACCAATATTAAAAGCTTTATCAA
GAACCGGCAACTCATCAAAAATATGACTACCGGCCGATTATCAAATGGTCCCGTCGAAGGTGTGAACCGTAAAATCAAGCAAATCAAAAGAACCGCGTAT
GGTTACAAGAACTGGCAAAACTTTATTTATCGAATTCAAATTGAGTTCAAAATCAAAATCGAAAAAAAGAGCCCAATTCGTAAATGAATTGGATTCTAAA
TATAACTTCATCAACACCGGTTGACAGAGACCC
ACCAATTGAAAGCGAGTGTCAGATATGTCCCAAGACCATTCTACTAAAGTACTGCTCCAAATTAAAGACCCAAATATTACTGATTTAGTTGTTCAAGAAG
CTACTAACGGAAGCTTACAAGTGCTTGCGAAGCTAACGTATCCGGTTACTGTTTGCGCACGTTGTGGCAAACGTTCCATGGTTAAGAATGGCTTTAAGAC
CGTTCACATCAGAATTCCTAGAATTAGCGAACGGGTCACCATCCTTAACTTAAAGAAGCAGCGATTTATCTGTAAAAATTGTGGTAAAAGTAGTTTAACA
ACGACTACGCTGGTTAAAAAACATCATCAAATATCTGAAAACAGTCTTCATGCCATTGATCTATCATTAACTGAAGACCGTACGATGGCGAGTATTGCGG
CTCAATACAATGTCTCGACCAATACGATTAGTCGCCGAATAAAGTTGCTCGGACAAGGTACCCAACCAGCTTTCAACGGACTACCAACGATGCTTTGTAT
CGACGAGTTTCGTTCAACTGATAAACAAATGAGTTTTATTGCGGTGGATGCCCAAACCCACGACATCATTACAATATTACCAGGACGTAAGAATGCCGAT
ATTAAAGACTTCTTTCTCAATCATTATTCTAAACGTCATCGTGACCGCGTAACCCGGGTTGTGATGGACTTTAATTCTCAATATCAACCCGCGATTCGAA
CACTCTTTCCACACGCCAAACTGGTCGCGGATAACTTTCATCTTGTTCAAATGGCCTTACGATCTTTGAATCAAACTCGGGTCCAACTCATGAAACGCTT
TAACCCTAAGAGTCGTGAATACCGAGTGCTTAAATATTATTGGCGGTTATATTTAAAAGATTATGACAGCCTCGAAAAGCGCAAACCCCAATGGTTTGCG
CATCTTAAAGATCGCATGACACAAGAGCAATTAGTCGTAGCCGGTTTAAATCTAGACCAACAGTTTCGACAAACCTACCAAGCCGCACATGCCTTAGTTA
AGGCTTTTCAAAAGCGTGATTATCGATCCTTCAAAGCAGCACTAGGTCATGTCGAAAATATCAGTCCTCAGCTAATGACCAATATTAAAAGCTTTATCAA
GAACCGGCAACTCATCAAAAATATGACTACCGGCCGATTATCAAATGGTCCCGTCGAAGGTGTGAACCGTAAAATCAAGCAAATCAAAAGAACCGCGTAT
GGTTACAAGAACTGGCAAAACTTTATTTATCGAATTCAAATTGAGTTCAAAATCAAAATCGAAAAAAAGAGCCCAATTCGTAAATGAATTGGATTCTAAA
TATAACTTCATCAACACCGGTTGACAGAGACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1263 bp | 420 aa | 125 | 1387 | + | No |
Chemistry : Unknow
ORF sequence :
MSQDHSTKVLLQIKDPNITDLVVQEATNGSLQVLAKLTYPVTVCARCGKRSMVKNGFKTVHIRIPRISERVTILNLKKQRFICKNCGKSSLTTTTLVKKH
HQISENSLHAIDLSLTEDRTMASIAAQYNVSTNTISRRIKLLGQGTQPAFNGLPTMLCIDEFRSTDKQMSFIAVDAQTHDIITILPGRKNADIKDFFLNH
YSKRHRDRVTRVVMDFNSQYQPAIRTLFPHAKLVADNFHLVQMALRSLNQTRVQLMKRFNPKSREYRVLKYYWRLYLKDYDSLEKRKPQWFAHLKDRMTQ
EQLVVAGLNLDQQFRQTYQAAHALVKAFQKRDYRSFKAALGHVENISPQLMTNIKSFIKNRQLIKNMTTGRLSNGPVEGVNRKIKQIKRTAYGYKNWQNF
IYRIQIEFKIKIEKKSPIRK
HQISENSLHAIDLSLTEDRTMASIAAQYNVSTNTISRRIKLLGQGTQPAFNGLPTMLCIDEFRSTDKQMSFIAVDAQTHDIITILPGRKNADIKDFFLNH
YSKRHRDRVTRVVMDFNSQYQPAIRTLFPHAKLVADNFHLVQMALRSLNQTRVQLMKRFNPKSREYRVLKYYWRLYLKDYDSLEKRKPQWFAHLKDRMTQ
EQLVVAGLNLDQQFRQTYQAAHALVKAFQKRDYRSFKAALGHVENISPQLMTNIKSFIKNRQLIKNMTTGRLSNGPVEGVNRKIKQIKRTAYGYKNWQNF
IYRIQIEFKIKIEKKSPIRK
Blast result :
Comments
ISP1 is 52% aa similar to ISL3.
There are 8 complete copies (coordinates : 2940108-2941540 ; 2698220-2699652 ; 2335158-2333726 ; 1955090-1956522 ; 1404967-1403535 ; 1342196-1340764 ; 1176862-1175430 ; 968530-967098 ) and 2 interrupted copies (coordinates : 1814210(1)-1813692(519) ; 1824440(596)-1825277(1433) ; 1823930(1)-1824448
(519) ; 1811522(1001)-1811091(1433) ; 1813700(596)-1813317(979) )
There are 8 complete copies (coordinates : 2940108-2941540 ; 2698220-2699652 ; 2335158-2333726 ; 1955090-1956522 ; 1404967-1403535 ; 1342196-1340764 ; 1176862-1175430 ; 968530-967098 ) and 2 interrupted copies (coordinates : 1814210(1)-1813692(519) ; 1824440(596)-1825277(1433) ; 1823930(1)-1824448
(519) ; 1811522(1001)-1811091(1433) ; 1813700(596)-1813317(979) )
References
1] Kleerebezem,M., Boekhorst,J., van Kranenburg,R., Molenaar,D., Kuipers,O.P., Leer,R., Tarchini,R., Peters,S.A., Sandbrink,H.M., Fiers,M.W., Stiekema,W., Lankhorst,R.M., Bron,P.A., Hoffer,S.M., Groot,M.N., Kerkhoven,R., de Vries,M., Ursing,B., de Vos,W.M. and Siezen,R.J. (2004) Proc. Natl. Acad. Sci. U.S.A. 100 (4), 1990-1995