ISMae1
- Family ISNCY
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ577137 | ND | Microcystis aeruginosa | Microcystis aeruginosa PCC 7806 |
DNA section
IS Length : 1318 bp
Ends
IR Length : 3/4
IRL : CTGGCAGGCATTCTACCACCCCCCCCTTGCCCCACCCGAAGTCGGGTAGG
IRR : GTGGATTGTAGTCATGGATAAAATACCAAAGGACTCCCCAAGGTGATTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CTGGCAGGCATTCTACCACCCCCCCCTTGCCCCACCCGAAGTCGGGTAGGCAAGGGGGGGGTATCGCATCTCAAAAGCTATTGACAATCGGCTAATTTTG
TGATCCGTGTGGGCATTTCAAGTGATACCAGTCAATCAAAATAGTTACGAAATCTTAACATTTAGTCGATTTTTGTTTAACAGTTGAGATGAGAAATTCT
ACTCAAGTATGCCTGGATCGCCCTCTGGGTAAGCAACTCACACGAGGTTCATTTTGTCAAGGGTTGTTAAGATGTGTTTACCTTACCATGTTGCTTATTC
GGTATGAACTGTGTAATATACTTATTACTCTCGGCTCGCAGATCACTATAGATGGTGTTTTGACAACGGATAAAACCTACCCGAAGTCGAATGTAGTTCA
ACTTACGCCAGTACAGGCAGTGGGTGGCTTAATCCAAAAACACCATATGTGGAAAATGAGCGAACCGTCAGTTTATCAATAAAAATAATTGGCTCAAATC
AATCCTTAAACCTCTAGCTTGATCATGACTTTTCTGATAAGTATCTCTTTATGGCTCGTGGAGGGCCTACTCAAATATCAGCCTAGCACGGAGCAAAATC
CCCCCACTGGTTTGCCTGTATTTACTTGTCCTAGTTGTGGTTCTCACCATACTATTAAGAACGGTTATCTTCCTAAGGGGAAACCCAAACGTCACTGTCA
AGAATGTGGTCAACCGTTTGTGATCAATCCCACTAATAAAACTATCTCTCCCGATACCAAGACAATTAATCGATCAACTCTTGCTCGAACGAATTTCCTG
ACGAGGAATTGCCAGAGTAACAGGGGTAAATTGGTCATGTTTACAAAATTATGTCAACAATAGACTAGCGGCTGTACCCCGTCAAGTAAAGGTTTCGGAC
AAACCAAAAGGTAAATTGCTCATAGAATGTGATGAGATGTGCCCTTTTGTTTTTTCTAAGACGATAAAGGTCTATATTTGGCGGTTAATTGATAGAAAGA
CAGGGGAACTTATTGGTGGCCATTTAGGATATAGGAGCCGCCAATCAGCTAAAAAACCTTGGCCAAGTTTACCCAGTGTTTACCGACAATGTGCTGTTAC
TTACACTCATTTTTGGGAGTCCTATAAGACAGTAATTCCGAGTAAACGTCATCGAGAGGTAGAGCCAAGAAACTGGTCAAAGTAATCCCCTTGGAAGACT
TAATAATACCTTTCGACCAAGGATTTCTCTGCTGGTGAGAGAGAGCCTATCTTTCTCTAAAAAAATGGAGAATCACCTTGGGGAGTCCTTTGGTATTTTA
TCCATGACTACAATCCAC
TGATCCGTGTGGGCATTTCAAGTGATACCAGTCAATCAAAATAGTTACGAAATCTTAACATTTAGTCGATTTTTGTTTAACAGTTGAGATGAGAAATTCT
ACTCAAGTATGCCTGGATCGCCCTCTGGGTAAGCAACTCACACGAGGTTCATTTTGTCAAGGGTTGTTAAGATGTGTTTACCTTACCATGTTGCTTATTC
GGTATGAACTGTGTAATATACTTATTACTCTCGGCTCGCAGATCACTATAGATGGTGTTTTGACAACGGATAAAACCTACCCGAAGTCGAATGTAGTTCA
ACTTACGCCAGTACAGGCAGTGGGTGGCTTAATCCAAAAACACCATATGTGGAAAATGAGCGAACCGTCAGTTTATCAATAAAAATAATTGGCTCAAATC
AATCCTTAAACCTCTAGCTTGATCATGACTTTTCTGATAAGTATCTCTTTATGGCTCGTGGAGGGCCTACTCAAATATCAGCCTAGCACGGAGCAAAATC
CCCCCACTGGTTTGCCTGTATTTACTTGTCCTAGTTGTGGTTCTCACCATACTATTAAGAACGGTTATCTTCCTAAGGGGAAACCCAAACGTCACTGTCA
AGAATGTGGTCAACCGTTTGTGATCAATCCCACTAATAAAACTATCTCTCCCGATACCAAGACAATTAATCGATCAACTCTTGCTCGAACGAATTTCCTG
ACGAGGAATTGCCAGAGTAACAGGGGTAAATTGGTCATGTTTACAAAATTATGTCAACAATAGACTAGCGGCTGTACCCCGTCAAGTAAAGGTTTCGGAC
AAACCAAAAGGTAAATTGCTCATAGAATGTGATGAGATGTGCCCTTTTGTTTTTTCTAAGACGATAAAGGTCTATATTTGGCGGTTAATTGATAGAAAGA
CAGGGGAACTTATTGGTGGCCATTTAGGATATAGGAGCCGCCAATCAGCTAAAAAACCTTGGCCAAGTTTACCCAGTGTTTACCGACAATGTGCTGTTAC
TTACACTCATTTTTGGGAGTCCTATAAGACAGTAATTCCGAGTAAACGTCATCGAGAGGTAGAGCCAAGAAACTGGTCAAAGTAATCCCCTTGGAAGACT
TAATAATACCTTTCGACCAAGGATTTCTCTGCTGGTGAGAGAGAGCCTATCTTTCTCTAAAAAAATGGAGAATCACCTTGGGGAGTCCTTTGGTATTTTA
TCCATGACTACAATCCAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 339 bp | 112 aa | 525 | 863 | + | No |
Description : First part of the transposase
ORF sequence :
MTFLISISLWLVEGLLKYQPSTEQNPPTGLPVFTCPSCGSHHTIKNGYLPKGKPKRHCQECGQPFVINPTNKTISPDTKTINRSTLARTNFLTRNCQSNR
GKLVMFTKLCQQ
GKLVMFTKLCQQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 321 bp | 219 aa | 525 | 845 | + | No |
Chemistry : Unknow
ORF sequence :
MTFLISISLWLVEGLLKYQPSTEQNPPTGLPVFTCPSCGSHHTIKNGYLPKGKPKRHCQECGQPFVINPTNKTISPDTKTINRSTLARTNFLTRNCQSNR
GKLVMFTNYVNNRLAAVPRQVKVSDKPKGKLLIECDEMCPFVFSKTIKVYIWRLIDRKTGELIGGHLGYRSRQSAKKPWPSLPSVYRQCAVTYTHFWESY
KTVIPSKRHREVEPRNWSK
GKLVMFTNYVNNRLAAVPRQVKVSDKPKGKLLIECDEMCPFVFSKTIKVYIWRLIDRKTGELIGGHLGYRSRQSAKKPWPSLPSVYRQCAVTYTHFWESY
KTVIPSKRHREVEPRNWSK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1 bp | 0 aa | 0 | 0 | - | No |
Chemistry : Unknow
ORF sequence :
Blast result :
Comments
ISMae1 was isolated as an insertion into the gvp genes to produced spontaneous GV-deficient mutants.
IsmA (ORF1) and IsmB may be fused by translational frameshift into the putative transposase IsmAB'(ORF2) ribosomal slippage.
IsmA is similar to the N terminal part of the IS1 family transposase but there is no DDE in the C terminal part of IsmAB'.
IsmA (ORF1) and IsmB may be fused by translational frameshift into the putative transposase IsmAB'(ORF2) ribosomal slippage.
IsmA is similar to the N terminal part of the IS1 family transposase but there is no DDE in the C terminal part of IsmAB'.
References
1] Mlouka,A., Comte,K., Castets,A.M., Bouchier,C. and Tandeau De Marsac,N. (2004) J. Bacteriol. 186 (8), 2355-2365