ISMae2
- Family ISNCY
- Group ISMae2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ577138 | ND | Microcystis aeruginosa | Microcystis aeruginosa PCC 7806 |
DNA section
IS Length : 1865 bp
Ends
IR Length : 24/25
IRL : CAGCAATTCTCATTTTGAAATAAATAGAGCATTAACCCCGATTTTGGCAT
IRR : CAGCAATTCTCATTTTGAAAAAAATTCAGATAAAATTCTCTTATTTAAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGAATTGACA | GGAATAAAA | CGAAGTCCTAC | 9 |
DNA sequence
CAGCAATTCTCATTTTGAAATAAATAGAGCATTAACCCCGATTTTGGCATGGGCATATAGTACAAAGTAACTATTTTTGAGGAGAGGCGATCACCCTAAA
AAACAGTTGGCTGGACAAATTAAGTATAAATACTCAAGCAATCAAAGAGGTTGGCTGAGAACAAACGGGGTTAATTCAGGAAGATAGTTCTGTTTTTAGA
ATAGGGAATTCAAGGGAAATAAAAATTTATAATTATTGTCAGCTAAGGTAAAATGGGTGAGATAGAAATCCACCAGCACGTCAAATTATTCCCAGATGGC
TCAAAAAGCGGCAACCCTAGAAATTTCGGAATTAATGCAATTTTTACGTCAAGAATTAGATGATTTACCCGATGAGAGGAAGCCTGGAAATAATAGAAAA
TATGAGGTAGAAGATGCCGTGATGGCTGCATTTTCAGTCTTTTTTACTCAGTCGCCGTCTTTTTTAGACCATCAACGTTTAATGAAAAGCAATAAGGGAA
AAGATAATGCTGAAAGTTTATTTTCAATTAAAAAAATTCCTGGGGATAATCAAATAAGAAATTTGTTAGATCCAGTGCCAGCATCTAATGTCTCTAGGAC
TTTTCAAAAAGTCTATGAATGGTTAAAGGAAAAGGGAGTTTTGAAAAAGTTTCTCTACTTAGATGGAGAAATGTTAGTGGCGTTAGATGGCACAGAATAT
TTCTCATCCAAGAAAATTAATTGCCCTCATTGTAACTGTCGTAATCATCGAAATGGAACTACAACTTATTTTCATGGCTGTGTCACACCGATGGTGGTTT
CTCCTAAGCAAAAACAAGTAATTAATTTAGAGCCAGAATTCATTAAAAAACAAGATGGACATCAAAAACAAGACTGTGAAAATGCGGCCGTTAAAAGATG
GTTAAATAGGAATCATAAAAATAAGTATGGTTATCCTGTAACTATTTTAGGAGACGACTTATATTCTCACGAACCTGTTTGTGAATTAGCGGTAAAACAA
GGTTATAATTTTATTTTTGTTGGTTTAGAAACGTCGCATAAAACTTTATATGAATGGCTAGAATTTTTAGAAAAAAGTGGCGAAGTTAGGACCGTTGAAA
AGAAACAATGGGATGGGCGAAAAAATCTAATTTATCGTTATCGTTATGCTTCTAGAGTTCCCTTAAGAGAGGGCGACTCAAGTCTCGAAGTTAATTGGTG
TGAAGTCACGGTCATTAATGAAAAAACCCAGAAAACTATCTACCAAAATAATTGGATAACTAATCATAAAATCACGGAAAATAATGTCGAAGAAATTGTC
AAAGCTGGACGCAGCAGATGGAAAGTTGAAAATGAAGGCAATAACGTTCTTAAAAATCACGGTTATAATTTAGAGCATAACTTTGGTCATGGTCAGAATC
ATTTGTGCGAGTTCTTATTGTCTTTGAATTTACTAGCTTTTTTATTTCATACCGTTTTGGATTTAGTTAATTACACCTATCAAAAGATTCGTGAGCTATT
AGTAACTCGGACTTCTTCTTTAATGATATTCGTACCCTATTAAAATTTATTTGGTTTAAGAATTGGTCGGAATTTTTCTCAGTTATTCTAACGGGATATG
TCCCATTTAAAAAAGTAAATTCTAGTTGAAAATGTCAGAACTCTTGAAGGAGAAAAAGAAAGTTGTTTATAAAAAAACTAAAAATTTTGTTAGAATTTCA
TCCAGATTTATGAGTTGAAATCGATTTTTCATGGACAAAGTTCTTAAAACCTGTCTATCTCCAGCGTTGGATGATAATTATCTGTCAAAAAATAATGCTT
CTACACAAGTTCTCTATTTAAATAAGAGAATTTTATCTGAATTTTTTTCAAAATGAGAATTGCTG
AAACAGTTGGCTGGACAAATTAAGTATAAATACTCAAGCAATCAAAGAGGTTGGCTGAGAACAAACGGGGTTAATTCAGGAAGATAGTTCTGTTTTTAGA
ATAGGGAATTCAAGGGAAATAAAAATTTATAATTATTGTCAGCTAAGGTAAAATGGGTGAGATAGAAATCCACCAGCACGTCAAATTATTCCCAGATGGC
TCAAAAAGCGGCAACCCTAGAAATTTCGGAATTAATGCAATTTTTACGTCAAGAATTAGATGATTTACCCGATGAGAGGAAGCCTGGAAATAATAGAAAA
TATGAGGTAGAAGATGCCGTGATGGCTGCATTTTCAGTCTTTTTTACTCAGTCGCCGTCTTTTTTAGACCATCAACGTTTAATGAAAAGCAATAAGGGAA
AAGATAATGCTGAAAGTTTATTTTCAATTAAAAAAATTCCTGGGGATAATCAAATAAGAAATTTGTTAGATCCAGTGCCAGCATCTAATGTCTCTAGGAC
TTTTCAAAAAGTCTATGAATGGTTAAAGGAAAAGGGAGTTTTGAAAAAGTTTCTCTACTTAGATGGAGAAATGTTAGTGGCGTTAGATGGCACAGAATAT
TTCTCATCCAAGAAAATTAATTGCCCTCATTGTAACTGTCGTAATCATCGAAATGGAACTACAACTTATTTTCATGGCTGTGTCACACCGATGGTGGTTT
CTCCTAAGCAAAAACAAGTAATTAATTTAGAGCCAGAATTCATTAAAAAACAAGATGGACATCAAAAACAAGACTGTGAAAATGCGGCCGTTAAAAGATG
GTTAAATAGGAATCATAAAAATAAGTATGGTTATCCTGTAACTATTTTAGGAGACGACTTATATTCTCACGAACCTGTTTGTGAATTAGCGGTAAAACAA
GGTTATAATTTTATTTTTGTTGGTTTAGAAACGTCGCATAAAACTTTATATGAATGGCTAGAATTTTTAGAAAAAAGTGGCGAAGTTAGGACCGTTGAAA
AGAAACAATGGGATGGGCGAAAAAATCTAATTTATCGTTATCGTTATGCTTCTAGAGTTCCCTTAAGAGAGGGCGACTCAAGTCTCGAAGTTAATTGGTG
TGAAGTCACGGTCATTAATGAAAAAACCCAGAAAACTATCTACCAAAATAATTGGATAACTAATCATAAAATCACGGAAAATAATGTCGAAGAAATTGTC
AAAGCTGGACGCAGCAGATGGAAAGTTGAAAATGAAGGCAATAACGTTCTTAAAAATCACGGTTATAATTTAGAGCATAACTTTGGTCATGGTCAGAATC
ATTTGTGCGAGTTCTTATTGTCTTTGAATTTACTAGCTTTTTTATTTCATACCGTTTTGGATTTAGTTAATTACACCTATCAAAAGATTCGTGAGCTATT
AGTAACTCGGACTTCTTCTTTAATGATATTCGTACCCTATTAAAATTTATTTGGTTTAAGAATTGGTCGGAATTTTTCTCAGTTATTCTAACGGGATATG
TCCCATTTAAAAAAGTAAATTCTAGTTGAAAATGTCAGAACTCTTGAAGGAGAAAAAGAAAGTTGTTTATAAAAAAACTAAAAATTTTGTTAGAATTTCA
TCCAGATTTATGAGTTGAAATCGATTTTTCATGGACAAAGTTCTTAAAACCTGTCTATCTCCAGCGTTGGATGATAATTATCTGTCAAAAAATAATGCTT
CTACACAAGTTCTCTATTTAAATAAGAGAATTTTATCTGAATTTTTTTCAAAATGAGAATTGCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1248 bp | 415 aa | 296 | 1543 | + | No |
Chemistry : Unknow
ORF sequence :
MAQKAATLEISELMQFLRQELDDLPDERKPGNNRKYEVEDAVMAAFSVFFTQSPSFLDHQRLMKSNKGKDNAESLFSIKKIPGDNQIRNLLDPVPASNVS
RTFQKVYEWLKEKGVLKKFLYLDGEMLVALDGTEYFSSKKINCPHCNCRNHRNGTTTYFHGCVTPMVVSPKQKQVINLEPEFIKKQDGHQKQDCENAAVK
RWLNRNHKNKYGYPVTILGDDLYSHEPVCELAVKQGYNFIFVGLETSHKTLYEWLEFLEKSGEVRTVEKKQWDGRKNLIYRYRYASRVPLREGDSSLEVN
WCEVTVINEKTQKTIYQNNWITNHKITENNVEEIVKAGRSRWKVENEGNNVLKNHGYNLEHNFGHGQNHLCEFLLSLNLLAFLFHTVLDLVNYTYQKIRE
LLVTRTSSLMIFVPY
RTFQKVYEWLKEKGVLKKFLYLDGEMLVALDGTEYFSSKKINCPHCNCRNHRNGTTTYFHGCVTPMVVSPKQKQVINLEPEFIKKQDGHQKQDCENAAVK
RWLNRNHKNKYGYPVTILGDDLYSHEPVCELAVKQGYNFIFVGLETSHKTLYEWLEFLEKSGEVRTVEKKQWDGRKNLIYRYRYASRVPLREGDSSLEVN
WCEVTVINEKTQKTIYQNNWITNHKITENNVEEIVKAGRSRWKVENEGNNVLKNHGYNLEHNFGHGQNHLCEFLLSLNLLAFLFHTVLDLVNYTYQKIRE
LLVTRTSSLMIFVPY
Blast result :
Comments
ISMae2 was described as a member of the IS4 family (Mlouka et al, 2004). BlastX analysis revealed a small homology with ISFac1 but no convincing DDE motif could be found.
ISMae2 was isolated as an insertion into the gvp genes to produced spontaneous GV-deficient mutants.
ISMae2 was isolated as an insertion into the gvp genes to produced spontaneous GV-deficient mutants.
References
1] Mlouka,A., Comte,K., Castets,A.M., Bouchier,C. and Tandeau De Marsac,N. (2004) J. Bacteriol. 186 (8), 2355-2365