ISPpa4
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY177682 | Y | Paracoccus pantotrophus | Paracoccus pantotrophus DSM 65 |
DNA section
IS Length : 1053 bp
Ends
IR Length : 13/15
IRL : GGCGTTGTTGCACAAGCCTGACGGCAAGTGATTCACTTTGCGAATCCATG
IRR : GGCGTTGTTGCGCAACTATCGCTTGAGGCGTGACTGCCCCTTCGCCCACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACCTTTACCA | AAGGTGATG | CGGAGAGATG | 9 |
DNA sequence
GGCGTTGTTGCACAAGCCTGACGGCAAGTGATTCACTTTGCGAATCCATGCCGTTAGACGCGCTGCATGAGCAAGCCATCACCCGCCCGCTATCGCACGA
CGAACTGGTCCAGCTACAACGCATCGCTGCGCAAGCGGGGGTCGCTGCTGATCTGGGTGGACACGGACATGACCTGGCTTGCGCCGCGTGATGGGAGGCC
TGGGAGGCCAGCGGTGTTTTCCGATGCGGCGATACAGTTCTGCCTGTCGATCAAGGTCCTGTTCAAGCTGCCGCTTCGCCAGACTGCCGGGATGGTGGCG
AGCCTGCTGAAGCTGGCGGGGCTGGACTGGCCGGTGCCTGACTTTTCCACGCTGTGCCGCCGACAGAAGACCCTGGCCGTGCAGATCCCCTATCGCCGCG
CCGACGGGCCGCTCAACCTGCTGGTCGACAGCACGGGCATCAAGTTCCTCGGCGACGGCGAATGGCAGGCGCGCAAGCACGGGGTGCAGGGGCGGCGCCA
ATGGCGCAAGGTGCATTTGGCGATGGACCCGGCCACGTCCGACATCCGCGCGGTGGAATTCACCCCTAGCCGCGACGGCGACAGCCCCGTTCTGCCCGAC
TTATTCGGCCAGATCCCGGCGGACGAGAAGATCGGCACGGTCACCGCCGACGGCGCTTATGACACGCGCCGCTGCCACACCGCTATCCTCGCGCGTGAGG
CCACCCCGATCATTCCGATCCGCAAGAACGGGCGGCTGTGGAAGGAAGATTGCCCGGCAGCACGCGCCCGAAACGACACCCTGCGCGCCACCCGACATTA
CGGACGGGCTTTCTGGAAACGGTGGACTGGATACCACGCCCGCAGCCGGATCGAGGCGAGGATGCGCTGCCTGAAATCCTTCGGCGAACGCATCATGGCG
AGAGACCCGGATCGCCAGACCGCCGACGTCCACATCCGCGTCGCACTCATGAACCGCTTCAACGCCCTCGGCACAGCCGACATCGTCCGCGTAGCCTGAG
TTGAGTGGGCGAAGGGGCAGTCACGCCTCAAGCGATAGTTGCGCAACAACGCC
CGAACTGGTCCAGCTACAACGCATCGCTGCGCAAGCGGGGGTCGCTGCTGATCTGGGTGGACACGGACATGACCTGGCTTGCGCCGCGTGATGGGAGGCC
TGGGAGGCCAGCGGTGTTTTCCGATGCGGCGATACAGTTCTGCCTGTCGATCAAGGTCCTGTTCAAGCTGCCGCTTCGCCAGACTGCCGGGATGGTGGCG
AGCCTGCTGAAGCTGGCGGGGCTGGACTGGCCGGTGCCTGACTTTTCCACGCTGTGCCGCCGACAGAAGACCCTGGCCGTGCAGATCCCCTATCGCCGCG
CCGACGGGCCGCTCAACCTGCTGGTCGACAGCACGGGCATCAAGTTCCTCGGCGACGGCGAATGGCAGGCGCGCAAGCACGGGGTGCAGGGGCGGCGCCA
ATGGCGCAAGGTGCATTTGGCGATGGACCCGGCCACGTCCGACATCCGCGCGGTGGAATTCACCCCTAGCCGCGACGGCGACAGCCCCGTTCTGCCCGAC
TTATTCGGCCAGATCCCGGCGGACGAGAAGATCGGCACGGTCACCGCCGACGGCGCTTATGACACGCGCCGCTGCCACACCGCTATCCTCGCGCGTGAGG
CCACCCCGATCATTCCGATCCGCAAGAACGGGCGGCTGTGGAAGGAAGATTGCCCGGCAGCACGCGCCCGAAACGACACCCTGCGCGCCACCCGACATTA
CGGACGGGCTTTCTGGAAACGGTGGACTGGATACCACGCCCGCAGCCGGATCGAGGCGAGGATGCGCTGCCTGAAATCCTTCGGCGAACGCATCATGGCG
AGAGACCCGGATCGCCAGACCGCCGACGTCCACATCCGCGTCGCACTCATGAACCGCTTCAACGCCCTCGGCACAGCCGACATCGTCCGCGTAGCCTGAG
TTGAGTGGGCGAAGGGGCAGTCACGCCTCAAGCGATAGTTGCGCAACAACGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 67 | 999 | + | No |
Chemistry : DDE
ORF sequence :
MSKPSPARYRTTNWSSYNASLRKRGSLLIWVDTDMTWLAPRDGRPGRPAVFSDAAIQFCLSIKVLFKLPLRQTAGMVASLLKLAGLDWPVPDFSTLCRRQ
KTLAVQIPYRRADGPLNLLVDSTGIKFLGDGEWQARKHGVQGRRQWRKVHLAMDPATSDIRAVEFTPSRDGDSPVLPDLFGQIPADEKIGTVTADGAYDT
RRCHTAILAREATPIIPIRKNGRLWKEDCPAARARNDTLRATRHYGRAFWKRWTGYHARSRIEARMRCLKSFGERIMARDPDRQTADVHIRVALMNRFNA
LGTADIVRVA
KTLAVQIPYRRADGPLNLLVDSTGIKFLGDGEWQARKHGVQGRRQWRKVHLAMDPATSDIRAVEFTPSRDGDSPVLPDLFGQIPADEKIGTVTADGAYDT
RRCHTAILAREATPIIPIRKNGRLWKEDCPAARARNDTLRATRHYGRAFWKRWTGYHARSRIEARMRCLKSFGERIMARDPDRQTADVHIRVALMNRFNA
LGTADIVRVA
Blast result :
Comments
ISPpa4 has been identified by its integration to the entrapment vector pMEC1. ISPpa4 is most similar to ISPpa3 of P. pantotrophus DSM 11072 (73 % of identity on the nucleotide sequence level). Hybridization analysis has shown that ISPpa4 is present in plasmid pHG16-a (approx. 70 kb) residing in the parental strain DSM 65.
ISPpa4 is 71% aa similar to ISAav2.
ISPpa4 is 71% aa similar to ISAav2.
References
1] Bartosik,D., Sochacka,M. and Baj,J. (2003) J. Bacteriol. 185 (13), 3753-3763
2] Bartosik,D.(2003) Direct submission
2] Bartosik,D.(2003) Direct submission