ISPpa2
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY179508 | Y | Paracoccus pantotrophus | Paracoccus pantotrophus DSM 11072 |
DNA section
IS Length : 832 bp
Ends
IR Length : 13/14
IRL : GGGTCAGGATGCATTGATTTTCGACGCGCTGCGTGATTCACGGCTCGGAA
IRR : GGGTCAGGATTCATAACCAGTAGATGACGACCGCGGCGAGGGCGATGGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACGTCGCCT | TA | AAGCAATTTA | 2 |
DNA sequence
GGGTCAGGATGCATTGATTTTCGACGCGCTGCGTGATTCACGGCTCGGAAAACGGAGCGGTGACATGAGCGACCTGTACTGGCTGACCAACGAGCAGATG
GCCAAGCTTGCCCCTTTTTTCCCCAGGTCCCACGGCAAGCCTCGGGTCGATGACAGACGGGTCTTGAGCGGAATTATTTTCATCAATCGCAATGGCTTGC
GCTGGCGCGACGCCCCTGAAGCCTACGGACCGCACAAGACGCTGTACAGCCGGTGGAAGCGTTGGAGCGAGAAAGGCATCTTCGCCCGGATGATGGTCGG
TCTGGCTGCGGAACACGGCGAGGAAAAGACCGTGATGATCGACGCGACGTATCTGAAAGCCCACCGCACTGCGACCAGCCTGGCCGCGAAAAAAGGGGGC
GTGGTCGCCTGATCGGTCGAACCAAGGGCGGCATGAACACCAAGCTGCACGCCATCTGCGACAGCCAAGGGCGACCGCTCAGCCTGTTCGTCACCGCTGG
ACAGGTCAGCGACTACATCGGTGCGCGGGCGTTACTGAGCAGTCTGCCCAAGGTCGACTGGCTTCTCGGAGATCGCGGCTATGACGCCGACTGGTTCCGA
GAGGCGTTGAAAGACAAGGGGATACGCGCCTGCATCCCTGGTAGAAAGCAGCGCAAGAAGGCCGTCAAATACGACAAGCGCCGATACAAACGGCGCAACC
GCATCGAGATCATGTTCGGCAGGCTCAAGGACTGGCGGCGTGTCGCGACACGCTATGATCGCTGTCCCAAGGTGTTCCTCTCAGCCATCGCCCTCGCCGC
GGTCGTCATCTACTGGTTATGAATCCTGACCC
GCCAAGCTTGCCCCTTTTTTCCCCAGGTCCCACGGCAAGCCTCGGGTCGATGACAGACGGGTCTTGAGCGGAATTATTTTCATCAATCGCAATGGCTTGC
GCTGGCGCGACGCCCCTGAAGCCTACGGACCGCACAAGACGCTGTACAGCCGGTGGAAGCGTTGGAGCGAGAAAGGCATCTTCGCCCGGATGATGGTCGG
TCTGGCTGCGGAACACGGCGAGGAAAAGACCGTGATGATCGACGCGACGTATCTGAAAGCCCACCGCACTGCGACCAGCCTGGCCGCGAAAAAAGGGGGC
GTGGTCGCCTGATCGGTCGAACCAAGGGCGGCATGAACACCAAGCTGCACGCCATCTGCGACAGCCAAGGGCGACCGCTCAGCCTGTTCGTCACCGCTGG
ACAGGTCAGCGACTACATCGGTGCGCGGGCGTTACTGAGCAGTCTGCCCAAGGTCGACTGGCTTCTCGGAGATCGCGGCTATGACGCCGACTGGTTCCGA
GAGGCGTTGAAAGACAAGGGGATACGCGCCTGCATCCCTGGTAGAAAGCAGCGCAAGAAGGCCGTCAAATACGACAAGCGCCGATACAAACGGCGCAACC
GCATCGAGATCATGTTCGGCAGGCTCAAGGACTGGCGGCGTGTCGCGACACGCTATGATCGCTGTCCCAAGGTGTTCCTCTCAGCCATCGCCCTCGCCGC
GGTCGTCATCTACTGGTTATGAATCCTGACCC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 65 | 412 | + | No |
Description : First part of the transposase
ORF sequence :
MSDLYWLTNEQMAKLAPFFPRSHGKPRVDDRRVLSGIIFINRNGLRWRDAPEAYGPHKTLYSRWKRWSEKGIFARMMVGLAAEHGEEKTVMIDATYLKAH
RTATSLAAKKGGVVA
RTATSLAAKKGGVVA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 570 bp | 189 aa | 253 | 822 | + | No |
Description : Second part of the transposase
ORF sequence :
MEALERERHLRPDDGRSGCGTRRGKDRDDRRDVSESPPHCDQPGREKRGRGRLIGRTKGGMNTKLHAICDSQGRPLSLFVTAGQVSDYIGARALLSSLPK
VDWLLGDRGYDADWFREALKDKGIRACIPGRKQRKKAVKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAAVVIYWL
VDWLLGDRGYDADWFREALKDKGIRACIPGRKQRKKAVKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAAVVIYWL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 758 bp | 0 aa | 65 | 822 | + | Yes |
Chemistry : DDE
ORF sequence :
Blast result :
Comments
References
1] Bartosik,D., Sochacka,M. and Baj,J. (2003) J. Bacteriol. 185 (13), 3753-3763
2] Bartosik,D.(2003) Direct submission
2] Bartosik,D.(2003) Direct submission