ISShdy1
- Family IS110
- Group IS1111
Isoform Synonym(s) ISSdy1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AIHI01000025 | ND | Shigella dysenteriae | Escherichia coli DEC13D gecDEC13D Shigella dysenteriae M131649 |
DNA section
IS Length : 1666 bp
Ends
IR Length : 9/10
IRL : gaatgaaAGGGACATCTTATTCGGTCTTTACGCTGGGGAGTTACTTTGGA
IRR : ---tatAA-GGACATCTGTTGTAGAGTCAACGATGATGATGAAATTAATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTCAAGCCGCTTTCGC | GGCATAGCCTTGCCGTC | 0 |
DNA sequence
GAATGAAAGGGACATCTTATTCGGTCTTTACGCTGGGGAGTTACTTTGGAAGACTGCGTCAACACGATGATAAGCGTACAAAGCCATTGTTAAGGGATCC
TCTATGACTGTTGTTACTGTCGGTATTGATTTAGCTAAAAATGTGTTCGCTGTGCATGGCGTTAATGCTGCAGGAAAAGCTGTTTTAGTTAAGCCTCTTG
TGCGTCGGGCTAAATTGCTTGAATTGATTGCAACACTTCCAGCATGTCTGATTGGAATTGAAGCATGTACAGGTGCACATTTCTGGGCAAGAGAGTTCCA
GAAAATGGGGCATACAGTTCGAATTATGGCGCCGCAACTGGTTTCTCCATACCGTATGGGTGGCCGTCATGGGAAAAATGATGCTGCAGATGCCGCTGCC
ATTTGTGAAGCTGTTACCCGTCCTAATATGCGGTTTGTTCCCATAAAATCAGTTGAGCAGCAAGGGCAGTTATTTGTACACAGAGCTCATCAGGGATATG
TCAGTGAGAGAACAGCATTGATTAATCGCATCCGGGGATTGCTCAGTGAGTTAGGTATTGTTCTCCCGCAGGCGTTTAAAGCGTTTTTATCCGGGGTGGC
TCAGGTCCTTGAGGATTTGCCCGGTTACTGTAATCAGGTTATTGGTGATCTTCTTAATGAGCTTTATCACATAGAAGAAAAGATTAAAAACAGTGAAAAA
CTCATTCAGCAACTTGCCAGAGATAACTCAGCAGCACAACTACTAATGCAATTCAGGGGGATAGGCCCGATGACTGCAACAGCAATCGTTGCCAGCATAG
GTAATGGTCATGATTTCAAGAACGGACGTCAGTTTAGTGCCTGGCTGGGGCTAACACCAAGACAACAAAGTTCCGGGGGGAAAGCACGGTTAGGGAGAAT
AACTAAAGCCGGCGACAGCTATCTCAGAACACTTCTTGTTTTGGGGGCGAGATCTGTATTGTTGGGAGCAGCTAAAAATGATGATCCGGTCAGTCGATGG
GCCCTGCAGTTAAAAGAAAGGCGAGGATATGGACGAGCGCTTGTTGCGATAGCAGCAAAAAATGCACGTATGTGCTGGGCAATGTTGCACCTGAGAGATG
AATTCAGATTTCCTGCCTAGTCTACTGATTATTAATGAGTTTATGAAATTGATTTCTTTCCTTGCATGACGACTGCACGATGATGATTTAAAAGGTTTGG
ACCTGCGCCATGGTTTACTCGAGATATGCAAGAGGCTGCCATGTTTATGTGGATATCTGTTTCGCGGATGGAGTCTATGGCGTGCGTCTTTCGTCAGGGT
TCGTTGCTAACAACAGCACGTTAATAACCGTTTATAGGCTCGCTGTCCGTAACCTTTTACTCTGACTGTGCAAGTTGTGTGCAGAAATCAATCTCATGAT
AGATAAAAGAATTATTCCACTGTTGCACCTGGAGTGTTCGGTGCTCGCTTTGCTGCGCTCCGCCTCAATCCAGTTGCAACAGCGTTTAATTAAAAGCATT
AAGGCTTGTGTCATTGCGTACTTGAGCTATGCAGGAGATTTTTATTGGTTCTCTGTTTCGCGGATGAAGTCTCTGACATGAGCCTTTCTCTCATAAAAAT
CGTTCTATTTCATATTGATATATTAATTTCATCATCATCGTTGACTCTACAACAGATGTCCTTATA
TCTATGACTGTTGTTACTGTCGGTATTGATTTAGCTAAAAATGTGTTCGCTGTGCATGGCGTTAATGCTGCAGGAAAAGCTGTTTTAGTTAAGCCTCTTG
TGCGTCGGGCTAAATTGCTTGAATTGATTGCAACACTTCCAGCATGTCTGATTGGAATTGAAGCATGTACAGGTGCACATTTCTGGGCAAGAGAGTTCCA
GAAAATGGGGCATACAGTTCGAATTATGGCGCCGCAACTGGTTTCTCCATACCGTATGGGTGGCCGTCATGGGAAAAATGATGCTGCAGATGCCGCTGCC
ATTTGTGAAGCTGTTACCCGTCCTAATATGCGGTTTGTTCCCATAAAATCAGTTGAGCAGCAAGGGCAGTTATTTGTACACAGAGCTCATCAGGGATATG
TCAGTGAGAGAACAGCATTGATTAATCGCATCCGGGGATTGCTCAGTGAGTTAGGTATTGTTCTCCCGCAGGCGTTTAAAGCGTTTTTATCCGGGGTGGC
TCAGGTCCTTGAGGATTTGCCCGGTTACTGTAATCAGGTTATTGGTGATCTTCTTAATGAGCTTTATCACATAGAAGAAAAGATTAAAAACAGTGAAAAA
CTCATTCAGCAACTTGCCAGAGATAACTCAGCAGCACAACTACTAATGCAATTCAGGGGGATAGGCCCGATGACTGCAACAGCAATCGTTGCCAGCATAG
GTAATGGTCATGATTTCAAGAACGGACGTCAGTTTAGTGCCTGGCTGGGGCTAACACCAAGACAACAAAGTTCCGGGGGGAAAGCACGGTTAGGGAGAAT
AACTAAAGCCGGCGACAGCTATCTCAGAACACTTCTTGTTTTGGGGGCGAGATCTGTATTGTTGGGAGCAGCTAAAAATGATGATCCGGTCAGTCGATGG
GCCCTGCAGTTAAAAGAAAGGCGAGGATATGGACGAGCGCTTGTTGCGATAGCAGCAAAAAATGCACGTATGTGCTGGGCAATGTTGCACCTGAGAGATG
AATTCAGATTTCCTGCCTAGTCTACTGATTATTAATGAGTTTATGAAATTGATTTCTTTCCTTGCATGACGACTGCACGATGATGATTTAAAAGGTTTGG
ACCTGCGCCATGGTTTACTCGAGATATGCAAGAGGCTGCCATGTTTATGTGGATATCTGTTTCGCGGATGGAGTCTATGGCGTGCGTCTTTCGTCAGGGT
TCGTTGCTAACAACAGCACGTTAATAACCGTTTATAGGCTCGCTGTCCGTAACCTTTTACTCTGACTGTGCAAGTTGTGTGCAGAAATCAATCTCATGAT
AGATAAAAGAATTATTCCACTGTTGCACCTGGAGTGTTCGGTGCTCGCTTTGCTGCGCTCCGCCTCAATCCAGTTGCAACAGCGTTTAATTAAAAGCATT
AAGGCTTGTGTCATTGCGTACTTGAGCTATGCAGGAGATTTTTATTGGTTCTCTGTTTCGCGGATGAAGTCTCTGACATGAGCCTTTCTCTCATAAAAAT
CGTTCTATTTCATATTGATATATTAATTTCATCATCATCGTTGACTCTACAACAGATGTCCTTATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 104 | 1120 | + | No |
Chemistry : DEDD
ORF sequence :
MTVVTVGIDLAKNVFAVHGVNAAGKAVLVKPLVRRAKLLELIATLPACLIGIEACTGAHFWAREFQKMGHTVRIMAPQLVSPYRMGGRHGKNDAADAAAI
CEAVTRPNMRFVPIKSVEQQGQLFVHRAHQGYVSERTALINRIRGLLSELGIVLPQAFKAFLSGVAQVLEDLPGYCNQVIGDLLNELYHIEEKIKNSEKL
IQQLARDNSAAQLLMQFRGIGPMTATAIVASIGNGHDFKNGRQFSAWLGLTPRQQSSGGKARLGRITKAGDSYLRTLLVLGARSVLLGAAKNDDPVSRWA
LQLKERRGYGRALVAIAAKNARMCWAMLHLRDEFRFPA
CEAVTRPNMRFVPIKSVEQQGQLFVHRAHQGYVSERTALINRIRGLLSELGIVLPQAFKAFLSGVAQVLEDLPGYCNQVIGDLLNELYHIEEKIKNSEKL
IQQLARDNSAAQLLMQFRGIGPMTATAIVASIGNGHDFKNGRQFSAWLGLTPRQQSSGGKARLGRITKAGDSYLRTLLVLGARSVLLGAAKNDDPVSRWA
LQLKERRGYGRALVAIAAKNARMCWAMLHLRDEFRFPA
Blast result :
Comments
IRs are not at the termini of the element.
The transposase protein is 61% identical to that of IS5708 and 40% identical to that of IS1111.
There is an empty site in Escherichia coli plasmid pND12_96 at position 18224 and in other Escherichia coli plasmids.
The transposase protein is 61% identical to that of IS5708 and 40% identical to that of IS1111.
There is an empty site in Escherichia coli plasmid pND12_96 at position 18224 and in other Escherichia coli plasmids.
References
1] The Welcome Trust Sanger Institute http://www.sanger.ac.uk
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
3] Hazen,T.H., Sahl,J.W., Redman,J.C., Morris,C.R., Daugherty,S.C., Chibucos,M.C., Sengamalay,N.A., Fraser-Liggett,C.M., Steinsland,H., Whittam,T.S., Whittam,B., Manning,S.D. and Rasko,D.A. (2012) J. Bacteriol. 194 (11), 3026-3027.
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
3] Hazen,T.H., Sahl,J.W., Redman,J.C., Morris,C.R., Daugherty,S.C., Chibucos,M.C., Sengamalay,N.A., Fraser-Liggett,C.M., Steinsland,H., Whittam,T.S., Whittam,B., Manning,S.D. and Rasko,D.A. (2012) J. Bacteriol. 194 (11), 3026-3027.