ISfinder Mirror Site

ISCc1

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_002696	ND	Caulobacter crescentus	Caulobacter crescentus CB15

DNA section

IS Length : 848 bp

Ends

IR Length : 13/18

IRL : GCCGTAGTGACGATTTAGGATTCCCGAGTTGGCCGAGGTCTGATTCAACA
IRR : GTGGCGGTGACCATTTAGCTATTTGAGCCAGAGCATGATGGCGGCGAGGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGAGCGGGTT	CTAG	AGCCCTTTCC	4
CTAAAGGGCT	CTAG	AGCGCCGTCA	4
TGCGACCGGA	CTAG	ACCGCGTTAG	4
CTAAAGGGCT	CTAG	CCGCCCCAGC	4
CTAACGCGCT	CTAG	AAGACGGGCT	4

DNA sequence

GCCGTAGTGACGATTTAGGATTCCCGAGTTGGCCGAGGTCTGATTCAACACTGCTTTTTGGAGGCGGTGTTGGAAGGCGAGGTTCTTCGGGACGACCAGT
GGGCGCGGATCGAGCTGTTCGTGCCCGGCGGTCGCAAGGGCAAGCGCGGCCCGCGCAGCAATGGCCGCCTGTTCGTGGACGCGCTGTTGTGGATGGCGCG
CTCGGGCGGGCGCTGGCGTGACCTGCCCGAGCGGTTTGGCCCCTACCAGACCGCCAAGCGCCGCTATTACCGCTGGATCGAGATGGGCGTGCTGAACCGC
CTGTTCGAGGCGGTGGCGGCCGAGCCCGACCTGGAATGGGCGATGCTGGACGCCACCGTCATCCGCGCCCACGCCCAAGCCGCTGGCGCGCGGCGAAAAA
GGGGGGACCGCACGCCCAAGCGCTTGGCCGTTCTCGCGGCGGCTTCGGAACCAAGATCCACGTCTGCGTAGACGCCCTGGGCCTGCCGGTCCGCATCGCC
CTGGGCCCTGGCCAGCAGAACGACATGGCCCCGGCCTGCGATCTGATCGACGGTCTGGCCGCCCAGAACGTCCTGGCCGATAAGGCCTATGACGCCGACC
GGCTCTACGAGAAGATCATCGCCCAGGGCGGCGATCCCGTCGTGCCGCCCCGCCGCCATCGCAAACGCCAGCACGCCTACGACGCGGTCCTCTACAAGGA
GAGAAACCGCGTCGAGCGTTTCTTCAGCCGCATCAAGCACTTCCGGCGCATCGCAACCCGATACGATAAGCTGGCGGCCAACTTCATGGGCTTCGTTACC
CTCGCCGCCATCATGCTCTGGCTCAAATAGCTAAATGGTCACCGCCAC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
402 bp	134 aa	67	468	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MLEGEVLRDDQWARIELFVPGGRKGKRGPRSNGRLFVDALLWMARSGGRWRDLPERFGPYQTAKRRYYRWIEMGVLNRLFEAVAAEPDLEWAMLDATVIR
AHAQAAGARRKRGDRTPKRLAVLAAASEPRSTSA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
546 bp	202 aa	282	827	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PARAVWPLPDRQAPLLPLDRDGRAEPPVRGGGGRARPGMGDAGRHRHPRPRPSRWRAAKKGGPHAQALGRSRGGFGTKIHVCVDALGLPVRIALGPGQQN
DMAPACDLIDGLAAQNVLADKAYDADRLYEKIIAQGGDPVVPPRRHRKRQHAYDAVLYKERNRVERFFSRIKHFRRIATRYDKLAANFMGFVTLAAIMLW
LK

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
761 bp	254 aa	67	827	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLEGEVLRDDQWARIELFVPGGRKGKRGPRSNGRLFVDALLWMARSGGRWRDLPERFGPYQTAKRRYYRWIEMGVLNRLFEAVAAEPDLEWAMLDATVIR
AHAQAAGARRKKGGPHAQALGRSRGGFGTKIHVCVDALGLPVRIALGPGQQNDMAPACDLIDGLAAQNVLADKAYDADRLYEKIIAQGGDPVVPPRRHRK
RQHAYDAVLYKERNRVERFFSRIKHFRRIATRYDKLAANFMGFVTLAAIMLWLK

Blast result :

Comments

The CB15 genome contains 5 copies: 1369620-1368773, 1392419-1393266, 2248617-2249464, 2802431-2803278, 3118089-3118936.
ISCc1 is 78%(ORF1) and 75% (ORF2) aa similar to ISNGR9.

References

1] Nierman, W.C., Feldblyum, T.V., Laub, M.T., Paulsen, I.T., Nelson, K.E., Eisen, J., Heidelberg, J.F., Alley, M.R.K., Ohta, N., Maddock, J.R., Potocka, I., Nelson, W.C., Newton, A., Stephens, C., Phadke, N.D., Ely, B., DeBoy, R.T., Dodson, R.J., Durkin, A.S., Gwinn, M.L., Haft, D.H., Kolonay, J.F., Smit, J., Craven, M.B., Khouri, H., Shetty, J., Berry, K., Utterback, T., Tran, K., Wolf, A., Vamathevan, J., Ermolaeva, M., White, O., Salzberg, S.L., Venter, J.C., Shapiro, L. and Fraser, C.M. PNAS 98: 4136-4141 (2001)