ISMma1
- Family ISH3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Goe1 |
DNA section
IS Length : 1500 bp
Ends
IR Length : 12
IRL : CAGTAGTTCCGAATATCCTTAGTTCCGAAACCTGTTTTTTACAATTGAAG
IRR : CAGTAGTTCCGATTTGTTATAGTTCCGAATGACAACTTTCATTCGTTTAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CAGTAGTTCCGAATATCCTTAGTTCCGAAACCTGTTTTTTACAATTGAAGGTATAATCAAAAAAAGTATTGAAGACTATGAAGTCGTTTGCTATGACTCC
AAAAAACGACTCCATGTCTGATGTAGACACAGCCAGTTTACTGGCTTTGTCTTCTGAATTCTTTAGCATCGTTGATATAATAACTTTACCCGATGGAGCA
AACTACAGCTATCAAGAGTTTCTTAACGTTTTACTTCATGCGGCAACATCTTCTACTGATTCTCTTGAATCTGCAAGTAATGATCTCAAATCAAAAGTTC
CAAACACAAGAATACCTTCAGCTGATACTATTTTCAATTACATAAAATCCAATTCTATTGAATATATCCTCTCTTCTTTCCGAAAAATAAATAATGAAAT
TTTTAGGATGATGAAGCTTAAAAACAATGTTCATGACATCGCTATTGACTTTCATGACGTTGAATATTATGGATGCAGAGACACTCTCTGTGTAAGGGGA
ATCAAACCAAAGAATGGAACTTCATGGGGATATTCTTTCTGCACCTTAGATGTAATTGGGAATTCTAAGCTAACACTTGATGTTATTGACATCAACGGAT
TAAGCAAAGATTATTCAATTCTTATGGAATCATTGTTTGAAAGGGTTGAAAAAATGGGAGTAAAAGTGGGAACAGTATACATGGATAGAGAATTTTTTAA
CAGAAAAGTTATCTCAAAGATGGAGAAATACAAAGTCGATTTCGTAATTGCAGCTAAATCAAACAAAAGAATTAAGGAGATGCTTGAAAGGCATAGAAAA
GAAAATAGAGATACTTCTACGGTTTTTGAATATAAGTTTCAAGGAGAAGAACAAACTTTTAACATTGTAGCAGTGTGGGATAAAGAAAAAGAATACAGTA
TATTTGCAACAAATAAAAAAGTGAGTTCAATAGACACATTTGTAAAGCAAATACCTGAAGAATACAGGAAGAGATGGAATATAGAAACAGGGTATAGAGT
AAAAAAGGATTTTAAGATACGAACATGTTCTAAATCACCTGTTGCAAGGACACTATTTTTTGTAGTTCAATGTATCATGTATAATATTTTGAATGTATTA
AAGTCAGTTCTTGACATTACAGCATACCAAATGAAATCAGTAATAAATCAAGACATTATTAAGGCAGTAAAAGAAGGAGTTAATTCATTATCCAATATTA
CAGTCAGGTCATTTCTTGAGTGCTTAACCAGATATAATAAAGAAAGAAGGCGAGCGCTTCGCGCTCGATTAAGAGATTTATAAATTTTTAAATATAACCA
AAAAAAAAAATCATAAAATTAGTTAGTGGAAACTATGCACAAATTTTACTTCAGGACTTTAAAATACTGAAGTTTTTAGAACATTATATAAAAAATGAAA
TCAGAATCGATGAGATGAAAACATTTTTCATGAACTATTAAGGAAAAATTGTAAACGAATGAAAGTTGTCATTCGGAACTATAACAAATCGGAACTACTG
AAAAAACGACTCCATGTCTGATGTAGACACAGCCAGTTTACTGGCTTTGTCTTCTGAATTCTTTAGCATCGTTGATATAATAACTTTACCCGATGGAGCA
AACTACAGCTATCAAGAGTTTCTTAACGTTTTACTTCATGCGGCAACATCTTCTACTGATTCTCTTGAATCTGCAAGTAATGATCTCAAATCAAAAGTTC
CAAACACAAGAATACCTTCAGCTGATACTATTTTCAATTACATAAAATCCAATTCTATTGAATATATCCTCTCTTCTTTCCGAAAAATAAATAATGAAAT
TTTTAGGATGATGAAGCTTAAAAACAATGTTCATGACATCGCTATTGACTTTCATGACGTTGAATATTATGGATGCAGAGACACTCTCTGTGTAAGGGGA
ATCAAACCAAAGAATGGAACTTCATGGGGATATTCTTTCTGCACCTTAGATGTAATTGGGAATTCTAAGCTAACACTTGATGTTATTGACATCAACGGAT
TAAGCAAAGATTATTCAATTCTTATGGAATCATTGTTTGAAAGGGTTGAAAAAATGGGAGTAAAAGTGGGAACAGTATACATGGATAGAGAATTTTTTAA
CAGAAAAGTTATCTCAAAGATGGAGAAATACAAAGTCGATTTCGTAATTGCAGCTAAATCAAACAAAAGAATTAAGGAGATGCTTGAAAGGCATAGAAAA
GAAAATAGAGATACTTCTACGGTTTTTGAATATAAGTTTCAAGGAGAAGAACAAACTTTTAACATTGTAGCAGTGTGGGATAAAGAAAAAGAATACAGTA
TATTTGCAACAAATAAAAAAGTGAGTTCAATAGACACATTTGTAAAGCAAATACCTGAAGAATACAGGAAGAGATGGAATATAGAAACAGGGTATAGAGT
AAAAAAGGATTTTAAGATACGAACATGTTCTAAATCACCTGTTGCAAGGACACTATTTTTTGTAGTTCAATGTATCATGTATAATATTTTGAATGTATTA
AAGTCAGTTCTTGACATTACAGCATACCAAATGAAATCAGTAATAAATCAAGACATTATTAAGGCAGTAAAAGAAGGAGTTAATTCATTATCCAATATTA
CAGTCAGGTCATTTCTTGAGTGCTTAACCAGATATAATAAAGAAAGAAGGCGAGCGCTTCGCGCTCGATTAAGAGATTTATAAATTTTTAAATATAACCA
AAAAAAAAAATCATAAAATTAGTTAGTGGAAACTATGCACAAATTTTACTTCAGGACTTTAAAATACTGAAGTTTTTAGAACATTATATAAAAAATGAAA
TCAGAATCGATGAGATGAAAACATTTTTCATGAACTATTAAGGAAAAATTGTAAACGAATGAAAGTTGTCATTCGGAACTATAACAAATCGGAACTACTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1206 bp | 401 aa | 78 | 1283 | + | No |
Chemistry : DDE
ORF sequence :
MKSFAMTPKNDSMSDVDTASLLALSSEFFSIVDIITLPDGANYSYQEFLNVLLHAATSSTDSLESASNDLKSKVPNTRIPSADTIFNYIKSNSIEYILSS
FRKINNEIFRMMKLKNNVHDIAIDFHDVEYYGCRDTLCVRGIKPKNGTSWGYSFCTLDVIGNSKLTLDVIDINGLSKDYSILMESLFERVEKMGVKVGTV
YMDREFFNRKVISKMEKYKVDFVIAAKSNKRIKEMLERHRKENRDTSTVFEYKFQGEEQTFNIVAVWDKEKEYSIFATNKKVSSIDTFVKQIPEEYRKRW
NIETGYRVKKDFKIRTCSKSPVARTLFFVVQCIMYNILNVLKSVLDITAYQMKSVINQDIIKAVKEGVNSLSNITVRSFLECLTRYNKERRRALRARLRD
L
FRKINNEIFRMMKLKNNVHDIAIDFHDVEYYGCRDTLCVRGIKPKNGTSWGYSFCTLDVIGNSKLTLDVIDINGLSKDYSILMESLFERVEKMGVKVGTV
YMDREFFNRKVISKMEKYKVDFVIAAKSNKRIKEMLERHRKENRDTSTVFEYKFQGEEQTFNIVAVWDKEKEYSIFATNKKVSSIDTFVKQIPEEYRKRW
NIETGYRVKKDFKIRTCSKSPVARTLFFVVQCIMYNILNVLKSVLDITAYQMKSVINQDIIKAVKEGVNSLSNITVRSFLECLTRYNKERRRALRARLRD
L
Blast result :
Comments
The transposase start codon is TTG.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baeumer,S., Jacobi,C., Brueggemann,H., Lienard,T., Christmann,A., Boemecke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.-P., Gunsalus,R.P., Fritz,H.-J. and Gottschalk,G. (2002)J. Mol. Microbiol. Biotechnol.